UniProtKB - P38144 (ISW1_YEAST)
Protein
ISWI chromatin-remodeling complex ATPase ISW1
Gene
ISW1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.4 Publications
Miscellaneous
Present with 1500 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 221 – 228 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA-dependent ATPase activity Source: GO_Central
- helicase activity Source: UniProtKB-KW
- nucleosome binding Source: InterPro
- nucleosome-dependent ATPase activity Source: InterPro
- rDNA binding Source: SGD
- transcription regulatory region sequence-specific DNA binding Source: SGD
GO - Biological processi
- ATP-dependent chromatin remodeling Source: GO_Central
- chromatin remodeling Source: SGD
- DNA-templated transcription, elongation Source: SGD
- heterochromatin maintenance Source: SGD
- negative regulation of histone exchange Source: SGD
- nucleosome positioning Source: GO_Central
- positive regulation of transcription by RNA polymerase II Source: SGD
- regulation of chromatin organization Source: SGD
- regulation of nucleosome density Source: SGD
- regulation of transcriptional start site selection at RNA polymerase II promoter Source: SGD
- sister chromatid cohesion Source: SGD
- termination of RNA polymerase II transcription Source: SGD
- termination of RNA polymerase I transcription Source: SGD
Keywordsi
Molecular function | Chromatin regulator, DNA-binding, Helicase, Hydrolase, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ISW1 Ordered Locus Names:YBR245C ORF Names:YBR1633 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000449, ISW1 |
VEuPathDBi | FungiDB:YBR245C |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- Isw1a complex Source: SGD
- Isw1b complex Source: SGD
- nucleolar chromatin Source: SGD
- nucleus Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 227 | K → A: Abolishes ATPase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074330 | 1 – 1129 | ISWI chromatin-remodeling complex ATPase ISW1Add BLAST | 1129 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 694 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 846 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P38144 |
PaxDbi | P38144 |
PRIDEi | P38144 |
PTM databases
iPTMneti | P38144 |
Interactioni
Subunit structurei
Component of the ISW1A complex, which at least consists of ISW1 and IOC3.
Component of the ISW1B complex, which at least consists of ISW1, IOC2 and IOC4.
2 PublicationsBinary interactionsi
Hide detailsP38144
With | #Exp. | IntAct |
---|---|---|
IOC2 [Q12072] | 6 | EBI-21087,EBI-30191 |
IOC3 [P43596] | 11 | EBI-21087,EBI-22944 |
Protein-protein interaction databases
BioGRIDi | 32940, 1864 interactors |
ComplexPortali | CPX-636, ISW1b chromatin remodeling complex CPX-637, ISW1a chromatin remodeling complex |
DIPi | DIP-6601N |
IntActi | P38144, 71 interactors |
MINTi | P38144 |
STRINGi | 4932.YBR245C |
Miscellaneous databases
RNActi | P38144, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P38144 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P38144 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 208 – 373 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 166 | |
Domaini | 506 – 657 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 152 | |
Domaini | 882 – 935 | SANT 1PROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 988 – 1052 | SANT 2PROSITE-ProRule annotationAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 324 – 327 | DEAH box | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0385, Eukaryota |
GeneTreei | ENSGT00940000176603 |
HOGENOMi | CLU_000315_0_2_1 |
InParanoidi | P38144 |
OMAi | QEIYYFR |
Family and domain databases
Gene3Di | 1.10.1040.30, 1 hit 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR009057, Homeobox-like_sf IPR015194, ISWI_HAND-dom IPR036306, ISWI_HAND-dom_sf IPR027417, P-loop_NTPase IPR001005, SANT/Myb IPR017884, SANT_dom IPR015195, SLIDE IPR038718, SNF2-like_sf IPR000330, SNF2_dom |
Pfami | View protein in Pfam PF09110, HAND, 1 hit PF00271, Helicase_C, 1 hit PF09111, SLIDE, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00717, SANT, 2 hits |
SUPFAMi | SSF101224, SSF101224, 1 hit SSF46689, SSF46689, 2 hits SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51293, SANT, 1 hit |
i Sequence
Sequence statusi: Complete.
P38144-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYMLAIANF HFFKFYTRMR KKHENNSCNE KDKDENLFKI ILAIFLQEKK
60 70 80 90 100
KYDCISSGSI MTASEEYLEN LKPFQVGLPP HDPESNKKRY LLKDANGKKF
110 120 130 140 150
DLEGTTKRFE HLLSLSGLFK HFIESKAAKD PKFRQVLDVL EENKANGKGK
160 170 180 190 200
GKHQDVRRRK TEHEEDAELL KEEDSDDDES IEFQFRESPA YVNGQLRPYQ
210 220 230 240 250
IQGVNWLVSL HKNKIAGILA DEMGLGKTLQ TISFLGYLRY IEKIPGPFLV
260 270 280 290 300
IAPKSTLNNW LREINRWTPD VNAFILQGDK EERAELIQKK LLGCDFDVVI
310 320 330 340 350
ASYEIIIREK SPLKKINWEY IIIDEAHRIK NEESMLSQVL REFTSRNRLL
360 370 380 390 400
ITGTPLQNNL HELWALLNFL LPDIFSDAQD FDDWFSSEST EEDQDKIVKQ
410 420 430 440 450
LHTVLQPFLL RRIKSDVETS LLPKKELNLY VGMSSMQKKW YKKILEKDLD
460 470 480 490 500
AVNGSNGSKE SKTRLLNIMM QLRKCCNHPY LFDGAEPGPP YTTDEHLVYN
510 520 530 540 550
AAKLQVLDKL LKKLKEEGSR VLIFSQMSRL LDILEDYCYF RNYEYCRIDG
560 570 580 590 600
STAHEDRIQA IDDYNAPDSK KFVFLLTTRA GGLGINLTSA DVVVLYDSDW
610 620 630 640 650
NPQADLQAMD RAHRIGQKKQ VKVFRLVTDN SVEEKILERA TQKLRLDQLV
660 670 680 690 700
IQQNRTSLKK KENKADSKDA LLSMIQHGAA DVFKSGTSTG SAGTPEPGSG
710 720 730 740 750
EKGDDIDLDE LLLKSENKTK SLNAKYETLG LDDLQKFNQD SAYEWNGQDF
760 770 780 790 800
KKKIQRDIIS PLLLNPTKRE RKENYSIDNY YKDVLNTGRS STPSHPRMPK
810 820 830 840 850
PHVFHSHQLQ PPQLKVLYEK ERMWTAKKTG YVPTMDDVKA AYGDISDEEE
860 870 880 890 900
KKQKLELLKL SVNNSQPLTE EEEKMKADWE SEGFTNWNKL EFRKFITVSG
910 920 930 940 950
KYGRNSIQAI ARELAPGKTL EEVRAYAKAF WSNIERIEDY EKYLKIIENE
960 970 980 990 1000
EEKIKRVKMQ QEALRRKLSE YKNPFFDLKL KHPPSSNNKR TYSEEEDRFI
1010 1020 1030 1040 1050
LLMLFKYGLD RDDVYELVRD EIRDCPLFEL DFYFRSRTPV ELARRGNTLL
1060 1070 1080 1090 1100
QCLEKEFNAG IVLDDATKDR MKKEDENGKR IREEFADQTA NEKENVDGVE
1110 1120
SKKAKIEDTS NVGTEQLVAE KIPENETTH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36114 Genomic DNA Translation: CAA85208.1 BK006936 Genomic DNA Translation: DAA07361.1 |
PIRi | S46122 |
RefSeqi | NP_009804.1, NM_001178593.1 |
Genome annotation databases
EnsemblFungii | YBR245C_mRNA; YBR245C; YBR245C |
GeneIDi | 852547 |
KEGGi | sce:YBR245C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36114 Genomic DNA Translation: CAA85208.1 BK006936 Genomic DNA Translation: DAA07361.1 |
PIRi | S46122 |
RefSeqi | NP_009804.1, NM_001178593.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Y9Y | X-ray | 3.25 | A | 763-1129 | [»] | |
2Y9Z | X-ray | 3.60 | A | 763-1129 | [»] | |
6IRO | electron microscopy | 3.40 | L | 69-1129 | [»] | |
6JYL | electron microscopy | 3.37 | K | 69-1129 | [»] | |
6K1P | electron microscopy | 3.87 | K | 69-1129 | [»] | |
SMRi | P38144 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32940, 1864 interactors |
ComplexPortali | CPX-636, ISW1b chromatin remodeling complex CPX-637, ISW1a chromatin remodeling complex |
DIPi | DIP-6601N |
IntActi | P38144, 71 interactors |
MINTi | P38144 |
STRINGi | 4932.YBR245C |
PTM databases
iPTMneti | P38144 |
Proteomic databases
MaxQBi | P38144 |
PaxDbi | P38144 |
PRIDEi | P38144 |
Genome annotation databases
EnsemblFungii | YBR245C_mRNA; YBR245C; YBR245C |
GeneIDi | 852547 |
KEGGi | sce:YBR245C |
Organism-specific databases
SGDi | S000000449, ISW1 |
VEuPathDBi | FungiDB:YBR245C |
Phylogenomic databases
eggNOGi | KOG0385, Eukaryota |
GeneTreei | ENSGT00940000176603 |
HOGENOMi | CLU_000315_0_2_1 |
InParanoidi | P38144 |
OMAi | QEIYYFR |
Miscellaneous databases
EvolutionaryTracei | P38144 |
PROi | PR:P38144 |
RNActi | P38144, protein |
Family and domain databases
Gene3Di | 1.10.1040.30, 1 hit 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR009057, Homeobox-like_sf IPR015194, ISWI_HAND-dom IPR036306, ISWI_HAND-dom_sf IPR027417, P-loop_NTPase IPR001005, SANT/Myb IPR017884, SANT_dom IPR015195, SLIDE IPR038718, SNF2-like_sf IPR000330, SNF2_dom |
Pfami | View protein in Pfam PF09110, HAND, 1 hit PF00271, Helicase_C, 1 hit PF09111, SLIDE, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00717, SANT, 2 hits |
SUPFAMi | SSF101224, SSF101224, 1 hit SSF46689, SSF46689, 2 hits SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51293, SANT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ISW1_YEAST | |
Accessioni | P38144Primary (citable) accession number: P38144 Secondary accession number(s): D6VQP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families