UniProtKB - P38121 (DPOA2_YEAST)
DNA polymerase alpha subunit B
POL12
Functioni
Non-catalytic component of DNA polymerase alpha, which in a complex with DNA primase (DNA polymerase alpha:primase) constitutes a replicative polymerase. POL12 may play an essential role at the early stage of chromosomal DNA replication by coupling DNA polymerase alpha to the cellular replication machinery (By similarity).
Interacts with MCM10.
By similarityMiscellaneous
GO - Molecular functioni
- DNA binding Source: InterPro
GO - Biological processi
- DNA biosynthetic process Source: GOC
- DNA replication initiation Source: SGD
- lagging strand elongation Source: SGD
- telomere capping Source: SGD
Keywordsi
Biological process | DNA replication |
Enzyme and pathway databases
Reactomei | R-SCE-113501, Inhibition of replication initiation of damaged DNA by RB1/E2F1 R-SCE-68952, DNA replication initiation R-SCE-68962, Activation of the pre-replicative complex R-SCE-69091, Polymerase switching R-SCE-69166, Removal of the Flap Intermediate R-SCE-69183, Processive synthesis on the lagging strand |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase alpha subunit BAlternative name(s): DNA polymerase I subunit B DNA polymerase alpha:primase complex p86 subunit Short name: Pol alpha-primase complex p86 subunit DNA polymerase-primase complex p74 subunit |
Gene namesi | Name:POL12 Ordered Locus Names:YBL035C ORF Names:YBL0414 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000131, POL12 |
VEuPathDBi | FungiDB:YBL035C |
Subcellular locationi
Nucleus
Nucleus
- alpha DNA polymerase:primase complex Source: SGD
- nuclear envelope Source: SGD
- nucleus Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194041 | 1 – 705 | DNA polymerase alpha subunit BAdd BLAST | 705 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 126 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P38121 |
PaxDbi | P38121 |
PRIDEi | P38121 |
PTM databases
iPTMneti | P38121 |
Interactioni
Subunit structurei
DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Subunit B binds to subunit A (By similarity).
By similarityBinary interactionsi
P38121
With | #Exp. | IntAct |
---|---|---|
POL1 [P13382] | 4 | EBI-6111,EBI-6128 |
STN1 [P38960] | 2 | EBI-6111,EBI-18427 |
Protein-protein interaction databases
BioGRIDi | 32662, 416 interactors |
ComplexPortali | CPX-2091, DNA polymerase alpha:primase complex |
DIPi | DIP-2536N |
IntActi | P38121, 30 interactors |
MINTi | P38121 |
STRINGi | 4932.YBL035C |
Miscellaneous databases
RNActi | P38121, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P38121 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P38121 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 115 – 199 | DisorderedSequence analysisAdd BLAST | 85 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 124 – 148 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 170 – 199 | Polar residuesSequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1625, Eukaryota |
GeneTreei | ENSGT00390000016784 |
HOGENOMi | CLU_014923_1_0_1 |
InParanoidi | P38121 |
OMAi | WVVGRIC |
Family and domain databases
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR016722, DNA_pol_alpha_bsu IPR013627, Pol_alpha_B_N |
PANTHERi | PTHR23061, PTHR23061, 1 hit |
Pfami | View protein in Pfam PF04042, DNA_pol_E_B, 1 hit PF08418, Pol_alpha_B_N, 1 hit |
PIRSFi | PIRSF018300, DNA_pol_alph_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSGSIDVITH FGPDADKPEI ITALENLTKL HALSVEDLYI KWEQFSNQRR
60 70 80 90 100
QTHTDLTSKN IDEFKQFLQL QMEKRANQIS SSSKVNTSTK KPVIKKSLNS
110 120 130 140 150
SPLFGLSIPK TPTLKKRKLH GPFSLSDSKQ TYNVGSEAET NEKGNSSLKL
160 170 180 190 200
EFTPGMAEDA VGDSAPLSHA KSSDAKTPGS STFQTPTTNT PTTSRQNVPA
210 220 230 240 250
GEILDSLNPE NIEISSGNPN VGLLSTEEPS YNQVKVEPFY DAKKYKFRTM
260 270 280 290 300
RQNLQEASDV LDDQIESFTK IIQNHYKLSP NDFADPTIQS QSEIYAVGRI
310 320 330 340 350
VPDSPTYDKF LNPESLSLET SRMGGVGRRV RLDLSQVNEL SFFLGQIVAF
360 370 380 390 400
KGKNANGDYF TVNSILPLPY PNSPVSTSQE LQEFQANLEG SSLKVIVTCG
410 420 430 440 450
PYFANDNFSL ELLQEFIDSI NNEVKPHVLI MFGPFIDITH PLIASGKLPN
460 470 480 490 500
FPQFKTQPKT LDELFLKLFT PILKTISPHI QTVLIPSTKD AISNHAAYPQ
510 520 530 540 550
ASLIRKALQL PKRNFKCMAN PSSFQINEIY FGCSNVDTFK DLKEVIKGGT
560 570 580 590 600
TSSRYRLDRV SEHILQQRRY YPIFPGSIRT RIKPKDVSTK KETNDMESKE
610 620 630 640 650
EKVYEHISGA DLDVSYLGLT EFVGGFSPDI MIIPSELQHF ARVVQNVVVI
660 670 680 690 700
NPGRFIRATG NRGSYAQITV QCPDLEDGKL TLVEGEEPVY LHNVWKRARV
DLIAS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35796 Genomic DNA Translation: CAA84855.1 X74738 Genomic DNA Translation: CAA52761.1 BK006936 Genomic DNA Translation: DAA07084.1 |
PIRi | S45769 |
RefSeqi | NP_009518.1, NM_001178275.1 |
Genome annotation databases
EnsemblFungii | YBL035C_mRNA; YBL035C; YBL035C |
GeneIDi | 852245 |
KEGGi | sce:YBL035C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35796 Genomic DNA Translation: CAA84855.1 X74738 Genomic DNA Translation: CAA52761.1 BK006936 Genomic DNA Translation: DAA07084.1 |
PIRi | S45769 |
RefSeqi | NP_009518.1, NM_001178275.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FLO | X-ray | 2.50 | A/C/E/G | 246-705 | [»] | |
SMRi | P38121 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32662, 416 interactors |
ComplexPortali | CPX-2091, DNA polymerase alpha:primase complex |
DIPi | DIP-2536N |
IntActi | P38121, 30 interactors |
MINTi | P38121 |
STRINGi | 4932.YBL035C |
PTM databases
iPTMneti | P38121 |
Proteomic databases
MaxQBi | P38121 |
PaxDbi | P38121 |
PRIDEi | P38121 |
Genome annotation databases
EnsemblFungii | YBL035C_mRNA; YBL035C; YBL035C |
GeneIDi | 852245 |
KEGGi | sce:YBL035C |
Organism-specific databases
SGDi | S000000131, POL12 |
VEuPathDBi | FungiDB:YBL035C |
Phylogenomic databases
eggNOGi | KOG1625, Eukaryota |
GeneTreei | ENSGT00390000016784 |
HOGENOMi | CLU_014923_1_0_1 |
InParanoidi | P38121 |
OMAi | WVVGRIC |
Enzyme and pathway databases
Reactomei | R-SCE-113501, Inhibition of replication initiation of damaged DNA by RB1/E2F1 R-SCE-68952, DNA replication initiation R-SCE-68962, Activation of the pre-replicative complex R-SCE-69091, Polymerase switching R-SCE-69166, Removal of the Flap Intermediate R-SCE-69183, Processive synthesis on the lagging strand |
Miscellaneous databases
EvolutionaryTracei | P38121 |
PROi | PR:P38121 |
RNActi | P38121, protein |
Family and domain databases
InterProi | View protein in InterPro IPR007185, DNA_pol_a/d/e_bsu IPR016722, DNA_pol_alpha_bsu IPR013627, Pol_alpha_B_N |
PANTHERi | PTHR23061, PTHR23061, 1 hit |
Pfami | View protein in Pfam PF04042, DNA_pol_E_B, 1 hit PF08418, Pol_alpha_B_N, 1 hit |
PIRSFi | PIRSF018300, DNA_pol_alph_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOA2_YEAST | |
Accessioni | P38121Primary (citable) accession number: P38121 Secondary accession number(s): D6VPW4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families