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Protein

5-aminolevulinate synthase, mitochondrial

Gene

hemA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.By similarity

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, mitochondrial (hemA)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei170SubstrateBy similarity1
Binding sitei283SubstrateBy similarity1
Binding sitei302SubstrateBy similarity1
Binding sitei335Pyridoxal phosphateBy similarity1
Binding sitei363Pyridoxal phosphateBy similarity1
Binding sitei409Pyridoxal phosphateBy similarity1
Active sitei412By similarity1
Binding sitei441Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei442Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei527SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processHeme biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, mitochondrial (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:hemA
ORF Names:AN2284
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VII
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000000124027 – 6485-aminolevulinate synthase, mitochondrialAdd BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei412N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi162425.CADANIAP00008976

Structurei

3D structure databases

ProteinModelPortaliP38092
SMRiP38092
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000221020
InParanoidiP38092
KOiK00643
OMAiKQDHTYR
OrthoDBiEOG092C1OK6

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR010961 4pyrrol_synth_NH2levulA_synth
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01821 5aminolev_synth, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38092-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEALLQQSRA MCPFLKRSSP NTLRSLATAT RPSTSPGGGT MTNLQRIARR
60 70 80 90 100
CPVMSKALAV QSARMTGTKR FTSSAAGVPG AGAGTPKPTR GSPGKRALHS
110 120 130 140 150
TGGNGANMST EFHKGAQQIH PGLSNATRSH VGASATVSGP TPRAPVAAPF
160 170 180 190 200
DYDAFYNAEL QKKHQDKSYR YFNNINRLAQ EFPRAHTASK DEKVTVWCSN
210 220 230 240 250
DYLGMGRNPE VLATMHKTLD TYGAGAGGTR NISGHNQHAV SLENTLAKLH
260 270 280 290 300
GKEAALVFSS CFVANDATLA TLGSKMPDCV ILSDSLNHAS MIQGIRHSGA
310 320 330 340 350
KKMVFKHNDL VDLETKLASL PLHVPKIIAF ESVYSMCGSI APIEAICDLA
360 370 380 390 400
DKYGAITFLD EVHAVGMYGP HGAGVAEHLD YEIYASQDTA NPLSTKGTVM
410 420 430 440 450
DRINIITGTL GKAYGCVGGY IAGSAALVDT IRSLAPGFIF TTSLPPATMA
460 470 480 490 500
GADTAIRYQA RHQQDRILQQ LHTRAVKQSF KDLDIPVIPN PSHIVPLLVG
510 520 530 540 550
DAELAKQASD KLLEEHGIYV QAINYPTVPR GEERLRITPT PGHTQELRDH
560 570 580 590 600
LVEAVNTVWN DLGIKRASDW KAMGGFVGVG VEAAELENQP IWTDAQLNMR
610 620 630 640
PDETLEAAVE REFQAAVPGM KAGGAKAKPV GSIAANPIGA SIPVAAAA
Length:648
Mass (Da):68,960
Last modified:May 26, 2009 - v2
Checksum:i1D450D773925F13F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300A → R in CAA45508 (PubMed:8319309).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64170 Genomic DNA Translation: CAA45508.1
AACD01000038 Genomic DNA Translation: EAA64395.1
BN001307 Genomic DNA Translation: CBF86531.1
PIRiS31846
RefSeqiXP_659888.1, XM_654796.1

Genome annotation databases

EnsemblFungiiCBF86531; CBF86531; ANIA_02284
EAA64395; EAA64395; AN2284.2
GeneIDi2874789
KEGGiani:AN2284.2

Similar proteinsi

Entry informationi

Entry nameiHEM1_EMENI
AccessioniPrimary (citable) accession number: P38092
Secondary accession number(s): C8VN31, Q5BAZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 26, 2009
Last modified: June 20, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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