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UniProtKB - P38071 (ETR1_YEAST)
Protein
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
Gene
ETR1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Required for respiration and the maintenance of the mitochondrial compartment.
2 PublicationsMiscellaneous
Present with 1560 molecules/cell in log phase SD medium.1 Publication
Caution
Was originally (PubMed:8195160) thought to be a nuclear protein involved in transcriptional regulation of genes required for the functional assembly of mitochondrial respiratory proteins. This was later proven not to be the case (PubMed:11509667).2 Publications
Catalytic activityi
- EC:1.3.1.1042 Publications
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 73 | Proton donorBy similarity | 1 | |
Binding sitei | 157 | NADPBy similarity | 1 | |
Binding sitei | 373 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 185 – 188 | NADPBy similarity | 4 | |
Nucleotide bindingi | 208 – 210 | NADPBy similarity | 3 | |
Nucleotide bindingi | 283 – 286 | NADPBy similarity | 4 | |
Nucleotide bindingi | 308 – 310 | NADPBy similarity | 3 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- enoyl-[acyl-carrier-protein] reductase activity Source: SGD
- trans-2-enoyl-CoA reductase (NADPH) activity Source: GO_Central
GO - Biological processi
- aerobic respiration Source: SGD
- fatty acid biosynthetic process Source: SGD
- fatty acid metabolic process Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.3.1.10, 984 |
Reactomei | R-SCE-77346, Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA |
Chemistry databases
SwissLipidsi | SLP:000001784 |
Names & Taxonomyi
Protein namesi | Recommended name: Enoyl-[acyl-carrier-protein] reductase, mitochondrialCurated (EC:1.3.1.1042 Publications)Alternative name(s): 2-enoyl thioester reductase1 Publication Mitochondrial respiratory function protein 11 Publication |
Gene namesi | Name:ETR1 Synonyms:MRF1, MRF1' Ordered Locus Names:YBR026C ORF Names:YBR0310 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000230, ETR1 |
VEuPathDBi | FungiDB:YBR026C |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 3 Publications
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | Y → N: 0.1% of catalytic activity. No specific ARS1 binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 9 | Mitochondrion1 Publication | 9 | |
ChainiPRO_0000160924 | 10 – 380 | Enoyl-[acyl-carrier-protein] reductase, mitochondrialAdd BLAST | 371 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 339 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P38071 |
PaxDbi | P38071 |
PRIDEi | P38071 |
PTM databases
iPTMneti | P38071 |
Expressioni
Inductioni
Positively regulated by the zinc-responsive transcriptional regulator ZAP1.1 Publication
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 32729, 400 interactors |
IntActi | P38071, 2 interactors |
MINTi | P38071 |
STRINGi | 4932.YBR026C |
Miscellaneous databases
RNActi | P38071, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0025, Eukaryota |
GeneTreei | ENSGT00940000156592 |
HOGENOMi | CLU_026673_17_0_1 |
InParanoidi | P38071 |
OMAi | HQLCRAW |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00107, ADH_zinc_N, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P38071-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLPTFKRYMS SSAHQIPKHF KSLIYSTHEV EDCTKVLSVK NYTPKQDLSQ
60 70 80 90 100
SIVLKTLAFP INPSDINQLQ GVYPSRPEKT YDYSTDEPAA IAGNEGVFEV
110 120 130 140 150
VSLPSGSSKG DLKLGDRVIP LQANQGTWSN YRVFSSSSDL IKVNDLDLFS
160 170 180 190 200
AATVSVNGCT GFQLVSDYID WNSNGNEWII QNAGTSSVSK IVTQVAKAKG
210 220 230 240 250
IKTLSVIRDR DNFDEVAKVL EDKYGATKVI SESQNNDKTF AKEVLSKILG
260 270 280 290 300
ENARVRLALN SVGGKSSASI ARKLENNALM LTYGGMSKQP VTLPTSLHIF
310 320 330 340 350
KGLTSKGYWV TEKNKKNPQS KIDTISDFIK MYNYGHIISP RDEIETLTWN
360 370 380
TNTTTDEQLL ELVKKGITGK GKKKMVVLEW
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | I → T in AAS56198 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26606 Genomic DNA Translation: BAA05651.1 Z35895 Genomic DNA Translation: CAA84968.1 X76078 Genomic DNA Translation: CAA53683.1 AY557872 Genomic DNA Translation: AAS56198.1 BK006936 Genomic DNA Translation: DAA07148.1 |
RefSeqi | NP_009582.1, NM_001178374.1 |
Genome annotation databases
EnsemblFungii | YBR026C_mRNA; YBR026C; YBR026C |
GeneIDi | 852314 |
KEGGi | sce:YBR026C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26606 Genomic DNA Translation: BAA05651.1 Z35895 Genomic DNA Translation: CAA84968.1 X76078 Genomic DNA Translation: CAA53683.1 AY557872 Genomic DNA Translation: AAS56198.1 BK006936 Genomic DNA Translation: DAA07148.1 |
RefSeqi | NP_009582.1, NM_001178374.1 |
3D structure databases
SMRi | P38071 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32729, 400 interactors |
IntActi | P38071, 2 interactors |
MINTi | P38071 |
STRINGi | 4932.YBR026C |
Chemistry databases
SwissLipidsi | SLP:000001784 |
PTM databases
iPTMneti | P38071 |
Proteomic databases
MaxQBi | P38071 |
PaxDbi | P38071 |
PRIDEi | P38071 |
Genome annotation databases
EnsemblFungii | YBR026C_mRNA; YBR026C; YBR026C |
GeneIDi | 852314 |
KEGGi | sce:YBR026C |
Organism-specific databases
SGDi | S000000230, ETR1 |
VEuPathDBi | FungiDB:YBR026C |
Phylogenomic databases
eggNOGi | KOG0025, Eukaryota |
GeneTreei | ENSGT00940000156592 |
HOGENOMi | CLU_026673_17_0_1 |
InParanoidi | P38071 |
OMAi | HQLCRAW |
Enzyme and pathway databases
BRENDAi | 1.3.1.10, 984 |
Reactomei | R-SCE-77346, Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA |
Miscellaneous databases
PROi | PR:P38071 |
RNActi | P38071, protein |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00107, ADH_zinc_N, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ETR1_YEAST | |
Accessioni | P38071Primary (citable) accession number: P38071 Secondary accession number(s): D6VQ28, Q6Q5P2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - SIMILARITY comments
Index of protein domains and families