UniProtKB - P38054 (CUSA_ECOLI)
Protein
Cation efflux system protein CusA
Gene
cusA
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of a cation efflux system that mediates resistance to copper and silver.2 Publications
Miscellaneous
The cus system plays an important role in copper tolerance under anaerobic growth and, under extreme copper stress, in aerobic growth.
GO - Molecular functioni
- copper ion binding Source: EcoCyc
- copper ion transmembrane transporter activity Source: EcoCyc
- silver ion transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- cellular copper ion homeostasis Source: EcoCyc
- copper ion export Source: EcoCyc
- detoxification of copper ion Source: EcoCyc
- plasma membrane copper ion transport Source: EcoCyc
- response to copper ion Source: EcoliWiki
- response to silver ion Source: EcoCyc
- silver ion transport Source: EcoCyc
Keywordsi
Biological process | Copper transport, Ion transport, Transport |
Ligand | Copper |
Enzyme and pathway databases
BioCyci | EcoCyc:YBDE-MONOMER MetaCyc:YBDE-MONOMER |
Protein family/group databases
TCDBi | 2.A.6.1.4, the resistance-nodulation-cell division (rnd) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cation efflux system protein CusA |
Gene namesi | Name:cusA Synonyms:ybdE Ordered Locus Names:b0575, JW0564 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 14 – 34 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 338 – 358 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 363 – 383 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 391 – 411 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 446 – 466 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 485 – 505 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 532 – 552 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 871 – 891 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 898 – 918 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 928 – 948 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 985 – 1005 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1012 – 1032 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 399 | A → D: Strong decrease in copper resistance. 1 Publication | 1 | |
Mutagenesisi | 405 | D → N: Loss of copper resistance. 1 Publication | 1 | |
Mutagenesisi | 412 | E → D: Slight decrease in copper resistance. 1 Publication | 1 | |
Mutagenesisi | 412 | E → Q: Loss of copper resistance. 1 Publication | 1 | |
Mutagenesisi | 573 | M → I: Loss of copper resistance. 1 Publication | 1 | |
Mutagenesisi | 623 | M → I: Loss of copper resistance. 1 Publication | 1 | |
Mutagenesisi | 640 | M → I: No change in copper resistance. 1 Publication | 1 | |
Mutagenesisi | 672 | M → I: Loss of copper resistance. 1 Publication | 1 | |
Mutagenesisi | 738 | M → I: No change in copper resistance. 1 Publication | 1 | |
Mutagenesisi | 755 | M → I: Slight decrease in copper resistance. 1 Publication | 1 | |
Mutagenesisi | 792 | M → I: No change in copper resistance. 1 Publication | 1 | |
Mutagenesisi | 812 | M → I: Slight decrease in copper resistance. 1 Publication | 1 | |
Mutagenesisi | 833 | M → I: Slight decrease in copper resistance. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161815 | 1 – 1047 | Cation efflux system protein CusAAdd BLAST | 1047 |
Proteomic databases
PaxDbi | P38054 |
PRIDEi | P38054 |
Expressioni
Inductioni
Transcriptionally regulated by CusR in response to copper and silver ions.1 Publication
Interactioni
Subunit structurei
The cus efflux system is composed of CusA, CusB, CusC and CusF.
Binary interactionsi
P38054
With | #Exp. | IntAct |
---|---|---|
cusB [P77239] | 2 | EBI-1126317,EBI-1118842 |
Protein-protein interaction databases
BioGRIDi | 4260908, 278 interactors |
ComplexPortali | CPX-2254, Cus cation efflux complex |
DIPi | DIP-9345N |
IntActi | P38054, 5 interactors |
STRINGi | 511145.b0575 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P38054 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P38054 |
Family & Domainsi
Sequence similaritiesi
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3696, Bacteria |
HOGENOMi | CLU_002755_1_2_6 |
InParanoidi | P38054 |
PhylomeDBi | P38054 |
Family and domain databases
Gene3Di | 3.30.2090.10, 2 hits |
InterProi | View protein in InterPro IPR027463, AcrB_DN_DC_subdom IPR001036, Acrflvin-R IPR004763, CzcA/CusA/SilA/NccA/HelA/CnrA |
PANTHERi | PTHR32063, PTHR32063, 1 hit |
Pfami | View protein in Pfam PF00873, ACR_tran, 1 hit |
PRINTSi | PR00702, ACRIFLAVINRP |
SUPFAMi | SSF82714, SSF82714, 2 hits |
TIGRFAMsi | TIGR00914, 2A0601, 1 hit |
i Sequence
Sequence statusi: Complete.
P38054-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEWIIRRSV ANRFLVLMGA LFLSIWGTWT IINTPVDALP DLSDVQVIIK
60 70 80 90 100
TSYPGQAPQI VENQVTYPLT TTMLSVPGAK TVRGFSQFGD SYVYVIFEDG
110 120 130 140 150
TDPYWARSRV LEYLNQVQGK LPAGVSAELG PDATGVGWIY EYALVDRSGK
160 170 180 190 200
HDLADLRSLQ DWFLKYELKT IPDVAEVASV GGVVKEYQVV IDPQRLAQYG
210 220 230 240 250
ISLAEVKSAL DASNQEAGGS SIELAEAEYM VRASGYLQTL DDFNHIVLKA
260 270 280 290 300
SENGVPVYLR DVAKVQIGPE MRRGIAELNG EGEVAGGVVI LRSGKNAREV
310 320 330 340 350
IAAVKDKLET LKSSLPEGVE IVTTYDRSQL IDRAIDNLSG KLLEEFIVVA
360 370 380 390 400
VVCALFLWHV RSALVAIISL PLGLCIAFIV MHFQGLNANI MSLGGIAIAV
410 420 430 440 450
GAMVDAAIVM IENAHKRLEE WQHQHPDATL DNKTRWQVIT DASVEVGPAL
460 470 480 490 500
FISLLIITLS FIPIFTLEGQ EGRLFGPLAF TKTYAMAGAA LLAIVVIPIL
510 520 530 540 550
MGYWIRGKIP PESSNPLNRF LIRVYHPLLL KVLHWPKTTL LVAALSVLTV
560 570 580 590 600
LWPLNKVGGE FLPQINEGDL LYMPSTLPGI SAAEAASMLQ KTDKLIMSVP
610 620 630 640 650
EVARVFGKTG KAETATDSAP LEMVETTIQL KPQEQWRPGM TMDKIIEELD
660 670 680 690 700
NTVRLPGLAN LWVPPIRNRI DMLSTGIKSP IGIKVSGTVL ADIDAMAEQI
710 720 730 740 750
EEVARTVPGV ASALAERLEG GRYINVEINR EKAARYGMTV ADVQLFVTSA
760 770 780 790 800
VGGAMVGETV EGIARYPINL RYPQSWRDSP QALRQLPILT PMKQQITLAD
810 820 830 840 850
VADIKVSTGP SMLKTENARP TSWIYIDARD RDMVSVVHDL QKAIAEKVQL
860 870 880 890 900
KPGTSVAFSG QFELLERANH KLKLMVPMTL MIIFVLLYLA FRRVGEALLI
910 920 930 940 950
ISSVPFALVG GIWLLWWMGF HLSVATGTGF IALAGVAAEF GVVMLMYLRH
960 970 980 990 1000
AIEAVPSLNN PQTFSEQKLD EALYHGAVLR VRPKAMTVAV IIAGLLPILW
1010 1020 1030 1040
GTGAGSEVMS RIAAPMIGGM ITAPLLSLFI IPAAYKLMWL HRHRVRK
Sequence cautioni
The sequence M58000 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 897 – 898 | AL → SV in M58000 (PubMed:1711024).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82598 Genomic DNA Translation: AAB40773.1 U00096 Genomic DNA Translation: AAC73676.1 AP009048 Genomic DNA Translation: BAA35215.1 M58000 Genomic DNA No translation available. |
PIRi | E64790 |
RefSeqi | NP_415107.1, NC_000913.3 WP_000573945.1, NZ_SSZK01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC73676; AAC73676; b0575 BAA35215; BAA35215; BAA35215 |
GeneIDi | 57730943 945191 |
KEGGi | ecj:JW0564 eco:b0575 |
PATRICi | fig|1411691.4.peg.1699 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82598 Genomic DNA Translation: AAB40773.1 U00096 Genomic DNA Translation: AAC73676.1 AP009048 Genomic DNA Translation: BAA35215.1 M58000 Genomic DNA No translation available. |
PIRi | E64790 |
RefSeqi | NP_415107.1, NC_000913.3 WP_000573945.1, NZ_SSZK01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3K07 | X-ray | 3.52 | A | 1-1047 | [»] | |
3K0I | X-ray | 4.12 | A | 1-1047 | [»] | |
3KSO | X-ray | 4.37 | A | 1-1047 | [»] | |
3KSS | X-ray | 3.88 | A | 1-1047 | [»] | |
3NE5 | X-ray | 2.90 | A | 2-1047 | [»] | |
3T51 | X-ray | 3.90 | A | 1-1047 | [»] | |
3T53 | X-ray | 3.37 | A | 1-1047 | [»] | |
3T56 | X-ray | 3.42 | A | 1-1047 | [»] | |
4DNR | X-ray | 3.68 | A | 1-1047 | [»] | |
4DNT | X-ray | 3.10 | A | 1-1047 | [»] | |
4DOP | X-ray | 4.20 | A | 1-1047 | [»] | |
SMRi | P38054 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260908, 278 interactors |
ComplexPortali | CPX-2254, Cus cation efflux complex |
DIPi | DIP-9345N |
IntActi | P38054, 5 interactors |
STRINGi | 511145.b0575 |
Protein family/group databases
TCDBi | 2.A.6.1.4, the resistance-nodulation-cell division (rnd) superfamily |
Proteomic databases
PaxDbi | P38054 |
PRIDEi | P38054 |
Genome annotation databases
EnsemblBacteriai | AAC73676; AAC73676; b0575 BAA35215; BAA35215; BAA35215 |
GeneIDi | 57730943 945191 |
KEGGi | ecj:JW0564 eco:b0575 |
PATRICi | fig|1411691.4.peg.1699 |
Organism-specific databases
EchoBASEi | EB2270 |
Phylogenomic databases
eggNOGi | COG3696, Bacteria |
HOGENOMi | CLU_002755_1_2_6 |
InParanoidi | P38054 |
PhylomeDBi | P38054 |
Enzyme and pathway databases
BioCyci | EcoCyc:YBDE-MONOMER MetaCyc:YBDE-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P38054 |
PROi | PR:P38054 |
Family and domain databases
Gene3Di | 3.30.2090.10, 2 hits |
InterProi | View protein in InterPro IPR027463, AcrB_DN_DC_subdom IPR001036, Acrflvin-R IPR004763, CzcA/CusA/SilA/NccA/HelA/CnrA |
PANTHERi | PTHR32063, PTHR32063, 1 hit |
Pfami | View protein in Pfam PF00873, ACR_tran, 1 hit |
PRINTSi | PR00702, ACRIFLAVINRP |
SUPFAMi | SSF82714, SSF82714, 2 hits |
TIGRFAMsi | TIGR00914, 2A0601, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CUSA_ECOLI | |
Accessioni | P38054Primary (citable) accession number: P38054 Secondary accession number(s): P77767 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families