Cation efflux system protein CusA

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• copper ion binding Source: EcoCyc <p>Inferred from Genetic Interaction</p> <p>Used to describe “traditional” genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • Ref.10

    "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
    Franke S., Grass G., Rensing C., Nies D.H.
    J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, MUTAGENESIS OF ALA-399; ASP-405; GLU-412; MET-573; MET-623; MET-640; MET-672; MET-738; MET-755; MET-792; MET-812 AND MET-833.
• copper ion transmembrane transporter activity Source: EcoCycInferred from genetic interactionⁱ
  • "Genes involved in copper homeostasis in Escherichia coli."
    Grass G., Rensing C.
    J. Bacteriol. 183:2145-2147(2001) [PubMed] [Europe PMC] [Abstract]
  • Ref.10

    "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
    Franke S., Grass G., Rensing C., Nies D.H.
    J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, MUTAGENESIS OF ALA-399; ASP-405; GLU-412; MET-573; MET-623; MET-640; MET-672; MET-738; MET-755; MET-792; MET-812 AND MET-833.
• silver ion transmembrane transporter activity Source: EcoCyc <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Diversity of silver resistance genes in IncH incompatibility group plasmids."
    Gupta A., Phung L.T., Taylor D.E., Silver S.
    Microbiology (Reading, Engl.) 147:3393-3402(2001) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• cellular copper ion homeostasis Source: EcoCycInferred from genetic interactionⁱ
  • "Genes involved in copper homeostasis in Escherichia coli."
    Grass G., Rensing C.
    J. Bacteriol. 183:2145-2147(2001) [PubMed] [Europe PMC] [Abstract]
• copper ion export Source: EcoCycInferred from genetic interactionⁱ
  • "Genes involved in copper homeostasis in Escherichia coli."
    Grass G., Rensing C.
    J. Bacteriol. 183:2145-2147(2001) [PubMed] [Europe PMC] [Abstract]
  • Ref.10

    "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
    Franke S., Grass G., Rensing C., Nies D.H.
    J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, MUTAGENESIS OF ALA-399; ASP-405; GLU-412; MET-573; MET-623; MET-640; MET-672; MET-738; MET-755; MET-792; MET-812 AND MET-833.
• detoxification of copper ion Source: EcoCycInferred from genetic interactionⁱ
  • "Genes involved in copper homeostasis in Escherichia coli."
    Grass G., Rensing C.
    J. Bacteriol. 183:2145-2147(2001) [PubMed] [Europe PMC] [Abstract]
  • Ref.10

    "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
    Franke S., Grass G., Rensing C., Nies D.H.
    J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, MUTAGENESIS OF ALA-399; ASP-405; GLU-412; MET-573; MET-623; MET-640; MET-672; MET-738; MET-755; MET-792; MET-812 AND MET-833.
• plasma membrane copper ion transport Source: EcoCycInferred from genetic interactionⁱ
  • "Genes involved in copper homeostasis in Escherichia coli."
    Grass G., Rensing C.
    J. Bacteriol. 183:2145-2147(2001) [PubMed] [Europe PMC] [Abstract]
  • Ref.10

    "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
    Franke S., Grass G., Rensing C., Nies D.H.
    J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, MUTAGENESIS OF ALA-399; ASP-405; GLU-412; MET-573; MET-623; MET-640; MET-672; MET-738; MET-755; MET-792; MET-812 AND MET-833.
• response to copper ion Source: EcoliWikiInferred from mutant phenotypeⁱ
  • Ref.10

    "Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli."
    Franke S., Grass G., Rensing C., Nies D.H.
    J. Bacteriol. 185:3804-3812(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, MUTAGENESIS OF ALA-399; ASP-405; GLU-412; MET-573; MET-623; MET-640; MET-672; MET-738; MET-755; MET-792; MET-812 AND MET-833.
• response to silver ion Source: EcoCycInferred from mutant phenotypeⁱ
  • Ref.9

    "The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions."
    Franke S., Grass G., Nies D.H.
    Microbiology 147:965-972(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: INDUCTION.
  • "Diversity of silver resistance genes in IncH incompatibility group plasmids."
    Gupta A., Phung L.T., Taylor D.E., Silver S.
    Microbiology (Reading, Engl.) 147:3393-3402(2001) [PubMed] [Europe PMC] [Abstract]
  • "Proteomic identification of the Cus system as a major determinant of constitutive Escherichia coli silver resistance of chromosomal origin."
    Lok C.N., Ho C.M., Chen R., Tam P.K., Chiu J.F., Che C.M.
    J. Proteome Res. 7:2351-2356(2008) [PubMed] [Europe PMC] [Abstract]
• silver ion transport Source: EcoCycInferred from mutant phenotypeⁱ
  • "Diversity of silver resistance genes in IncH incompatibility group plasmids."
    Gupta A., Phung L.T., Taylor D.E., Silver S.
    Microbiology (Reading, Engl.) 147:3393-3402(2001) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Topology

GO - Cellular componentⁱ

• integral component of membrane Source: EcoCyc <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport."
    Long F., Su C.C., Zimmermann M.T., Boyken S.E., Rajashankar K.R., Jernigan R.L., Yu E.W.
    Nature 467:484-488(2010) [PubMed] [Europe PMC] [Abstract]
• plasma membrane Source: EcoCycInferred from direct assayⁱ
  • "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductionⁱ

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MIEWIIRRSV ANRFLVLMGA LFLSIWGTWT IINTPVDALP DLSDVQVIIK 
        60         70         80         90        100
TSYPGQAPQI VENQVTYPLT TTMLSVPGAK TVRGFSQFGD SYVYVIFEDG 
       110        120        130        140        150
TDPYWARSRV LEYLNQVQGK LPAGVSAELG PDATGVGWIY EYALVDRSGK 
       160        170        180        190        200
HDLADLRSLQ DWFLKYELKT IPDVAEVASV GGVVKEYQVV IDPQRLAQYG 
       210        220        230        240        250
ISLAEVKSAL DASNQEAGGS SIELAEAEYM VRASGYLQTL DDFNHIVLKA 
       260        270        280        290        300
SENGVPVYLR DVAKVQIGPE MRRGIAELNG EGEVAGGVVI LRSGKNAREV 
       310        320        330        340        350
IAAVKDKLET LKSSLPEGVE IVTTYDRSQL IDRAIDNLSG KLLEEFIVVA 
       360        370        380        390        400
VVCALFLWHV RSALVAIISL PLGLCIAFIV MHFQGLNANI MSLGGIAIAV 
       410        420        430        440        450
GAMVDAAIVM IENAHKRLEE WQHQHPDATL DNKTRWQVIT DASVEVGPAL 
       460        470        480        490        500
FISLLIITLS FIPIFTLEGQ EGRLFGPLAF TKTYAMAGAA LLAIVVIPIL 
       510        520        530        540        550
MGYWIRGKIP PESSNPLNRF LIRVYHPLLL KVLHWPKTTL LVAALSVLTV 
       560        570        580        590        600
LWPLNKVGGE FLPQINEGDL LYMPSTLPGI SAAEAASMLQ KTDKLIMSVP 
       610        620        630        640        650
EVARVFGKTG KAETATDSAP LEMVETTIQL KPQEQWRPGM TMDKIIEELD 
       660        670        680        690        700
NTVRLPGLAN LWVPPIRNRI DMLSTGIKSP IGIKVSGTVL ADIDAMAEQI 
       710        720        730        740        750
EEVARTVPGV ASALAERLEG GRYINVEINR EKAARYGMTV ADVQLFVTSA 
       760        770        780        790        800
VGGAMVGETV EGIARYPINL RYPQSWRDSP QALRQLPILT PMKQQITLAD 
       810        820        830        840        850
VADIKVSTGP SMLKTENARP TSWIYIDARD RDMVSVVHDL QKAIAEKVQL 
       860        870        880        890        900
KPGTSVAFSG QFELLERANH KLKLMVPMTL MIIFVLLYLA FRRVGEALLI 
       910        920        930        940        950
ISSVPFALVG GIWLLWWMGF HLSVATGTGF IALAGVAAEF GVVMLMYLRH 
       960        970        980        990       1000
AIEAVPSLNN PQTFSEQKLD EALYHGAVLR VRPKAMTVAV IIAGLLPILW 
      1010       1020       1030       1040 
GTGAGSEVMS RIAAPMIGGM ITAPLLSLFI IPAAYKLMWL HRHRVRK    

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautionⁱ

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families
3. Escherichia coli
   Escherichia coli (strain K12): entries and cross-references to EcoGene