UniProtKB - P38051 (MENF_ECOLI)
Protein
Isochorismate synthase MenF
Gene
menF
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the conversion of chorismate to isochorismate. Can also catalyze the reverse reaction, but with a lower efficiency.4 Publications
Catalytic activityi
- EC:5.4.4.2UniRule annotation4 Publications
Cofactori
Mg2+UniRule annotation4 PublicationsNote: Binds more tightly in the presence of chorismate.1 Publication
Kineticsi
kcat is 213 min(-1) for mutase activity with chorismate. kcat is 176 min(-1) for mutase activity with chorismate in the presence of 30 mM of beta-mercaptoethanol (BME). kcat is 144.9 min(-1) for mutase activity with chorismate (at pH 7.5 and 37 degrees Celsius). kcat is 80 min(-1) for mutase activity with chorismate in the absence of 30 mM of beta-mercaptoethanol (BME). kcat is 3.8 min(-1) for mutase activity with isochorismate (at pH 7.5 and 37 degrees Celsius.3 Publications
- KM=119 µM for isochorismate (at pH 7.5 and 37 degrees Celsius)1 Publication
- KM=166.9 µM for chorismate (at pH 7.5 and 37 degrees Celsius)1 Publication
- KM=192 µM for chorismate1 Publication
- KM=195 µM for chorismate1 Publication
- KM=770 µM for magnesium1 Publication
- Vmax=91.7 pmol/sec/mg enzyme with chorismate as substrate (at pH 7.5 and 37 degrees Celsius)1 Publication
- Vmax=2.4 pmol/sec/mg enzyme with isochorismate as substrate (at pH 7.5 and 37 degrees Celsius)1 Publication
pH dependencei
Optimum pH is between 7.5 and 8. The mutase activity decreases sharply at pH 6.5 and 9 to about 20% of that observed at the optimum pH.2 Publications
Temperature dependencei
Optimum temperature is 37 degrees Celsius. At 60 degrees Celsius the mutase activity is completely lost.2 Publications
: 1,4-dihydroxy-2-naphthoate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation2 PublicationsProteins known to be involved in the 7 steps of the subpathway in this organism are:
- Isochorismate synthase MenF (menF), Isochorismate synthase MenF (menF)
- 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
- 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH), 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
- o-succinylbenzoate synthase (menC), o-succinylbenzoate synthase (menC)
- 2-succinylbenzoate--CoA ligase (menE), 2-succinylbenzoate--CoA ligase (menE)
- 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB), 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
- 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (menI), 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (menI)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.
Pathwayi: menaquinone biosynthesis
This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 190 | Proton acceptorUniRule annotation1 Publication | 1 | |
Active sitei | 240 | Proton donorUniRule annotation1 Publication | 1 | |
Metal bindingi | 284 | MagnesiumUniRule annotation1 Publication | 1 | |
Metal bindingi | 416 | MagnesiumUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- isochorismate synthase activity Source: EcoCyc
- magnesium ion binding Source: EcoCyc
GO - Biological processi
- menaquinone biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | Menaquinone biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MENF-MONOMER MetaCyc:MENF-MONOMER |
BRENDAi | 5.4.4.2, 2026 |
SABIO-RKi | P38051 |
UniPathwayi | UPA00079 UPA01057;UER00163 |
Names & Taxonomyi
Protein namesi | Recommended name: Isochorismate synthase MenF1 PublicationUniRule annotation (EC:5.4.4.2UniRule annotation4 Publications)Alternative name(s): Isochorismate hydroxymutase1 Publication Isochorismate mutaseUniRule annotation |
Gene namesi | Name:menFUniRule annotation Synonyms:yfbA Ordered Locus Names:b2265, JW2260 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutant produces only a trace of menaquinone.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 190 | K → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 240 | E → Q: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 255 | L → A: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 344 | A → T: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 387 | R → A: Lack of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154147 | 1 – 431 | Isochorismate synthase MenFAdd BLAST | 431 |
Proteomic databases
jPOSTi | P38051 |
PaxDbi | P38051 |
PRIDEi | P38051 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262080, 6 interactors |
DIPi | DIP-10187N |
IntActi | P38051, 7 interactors |
STRINGi | 511145.b2265 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P38051 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P38051 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1169, Bacteria |
HOGENOMi | CLU_006493_8_4_6 |
InParanoidi | P38051 |
PhylomeDBi | P38051 |
Family and domain databases
Gene3Di | 3.60.120.10, 1 hit |
HAMAPi | MF_01935, MenF, 1 hit |
InterProi | View protein in InterPro IPR005801, ADC_synthase IPR015890, Chorismate_C IPR004561, IsoChor_synthase IPR034681, MenF |
Pfami | View protein in Pfam PF00425, Chorismate_bind, 1 hit |
SUPFAMi | SSF56322, SSF56322, 1 hit |
TIGRFAMsi | TIGR00543, isochor_syn, 1 hit |
i Sequence
Sequence statusi: Complete.
P38051-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSLTTALEN LLRHLSQEIP ATPGIRVIDI PFPLKDAFDA LSWLASQQTY
60 70 80 90 100
PQFYWQQRNG DEEAVVLGAI TRFTSLDQAQ RFLRQHPEHA DLRIWGLNAF
110 120 130 140 150
DPSQGNLLLP RLEWRRCGGK ATLRLTLFSE SSLQHDAIQA KEFIATLVSI
160 170 180 190 200
KPLPGLHLTT TREQHWPDKT GWTQLIELAT KTIAEGELDK VVLARATDLH
210 220 230 240 250
FASPVNAAAM MAASRRLNLN CYHFYMAFDG ENAFLGSSPE RLWRRRDKAL
260 270 280 290 300
RTEALAGTVA NNPDDKQAQQ LGEWLMADDK NQRENMLVVE DICQRLQADT
310 320 330 340 350
QTLDVLPPQV LRLRKVQHLR RCIWTSLNKA DDVICLHQLQ PTAAVAGLPR
360 370 380 390 400
DLARQFIARH EPFTREWYAG SAGYLSLQQS EFCVSLRSAK ISGNVVRLYA
410 420 430
GAGIVRGSDP EQEWQEIDNK AAGLRTLLQM E
Sequence cautioni
The sequence M21787 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 310 – 312 | VLR → YC in AAC44303 (PubMed:8764478).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z50849 Genomic DNA Translation: CAA90702.1 U54790 Genomic DNA Translation: AAC44303.1 U00096 Genomic DNA Translation: AAC75325.2 AP009048 Genomic DNA Translation: BAA16092.1 U58768 Genomic DNA Translation: AAB02729.1 M21787 Genomic DNA No translation available. |
PIRi | G64997 |
RefSeqi | NP_416768.4, NC_000913.3 WP_001191419.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75325; AAC75325; b2265 BAA16092; BAA16092; BAA16092 |
GeneIDi | 946712 |
KEGGi | ecj:JW2260 eco:b2265 |
PATRICi | fig|1411691.4.peg.4471 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z50849 Genomic DNA Translation: CAA90702.1 U54790 Genomic DNA Translation: AAC44303.1 U00096 Genomic DNA Translation: AAC75325.2 AP009048 Genomic DNA Translation: BAA16092.1 U58768 Genomic DNA Translation: AAB02729.1 M21787 Genomic DNA No translation available. |
PIRi | G64997 |
RefSeqi | NP_416768.4, NC_000913.3 WP_001191419.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EUA | X-ray | 2.50 | A/B | 1-431 | [»] | |
3BZM | X-ray | 1.95 | A | 1-431 | [»] | |
3BZN | X-ray | 2.00 | A | 1-431 | [»] | |
SMRi | P38051 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262080, 6 interactors |
DIPi | DIP-10187N |
IntActi | P38051, 7 interactors |
STRINGi | 511145.b2265 |
Proteomic databases
jPOSTi | P38051 |
PaxDbi | P38051 |
PRIDEi | P38051 |
Genome annotation databases
EnsemblBacteriai | AAC75325; AAC75325; b2265 BAA16092; BAA16092; BAA16092 |
GeneIDi | 946712 |
KEGGi | ecj:JW2260 eco:b2265 |
PATRICi | fig|1411691.4.peg.4471 |
Organism-specific databases
EchoBASEi | EB2265 |
Phylogenomic databases
eggNOGi | COG1169, Bacteria |
HOGENOMi | CLU_006493_8_4_6 |
InParanoidi | P38051 |
PhylomeDBi | P38051 |
Enzyme and pathway databases
UniPathwayi | UPA00079 UPA01057;UER00163 |
BioCyci | EcoCyc:MENF-MONOMER MetaCyc:MENF-MONOMER |
BRENDAi | 5.4.4.2, 2026 |
SABIO-RKi | P38051 |
Miscellaneous databases
EvolutionaryTracei | P38051 |
PROi | PR:P38051 |
Family and domain databases
Gene3Di | 3.60.120.10, 1 hit |
HAMAPi | MF_01935, MenF, 1 hit |
InterProi | View protein in InterPro IPR005801, ADC_synthase IPR015890, Chorismate_C IPR004561, IsoChor_synthase IPR034681, MenF |
Pfami | View protein in Pfam PF00425, Chorismate_bind, 1 hit |
SUPFAMi | SSF56322, SSF56322, 1 hit |
TIGRFAMsi | TIGR00543, isochor_syn, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MENF_ECOLI | |
Accessioni | P38051Primary (citable) accession number: P38051 Secondary accession number(s): P76479 Q47704 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families