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Protein

Anaphase-promoting complex subunit CDC27

Gene

CDC27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.3 Publications

Miscellaneous

Present with 593 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: SGD
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • protein ubiquitination Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-28973-MONOMER
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit CDC27
Alternative name(s):
Anaphase-promoting complex subunit 3
Cell division control protein 27
Gene namesi
Name:CDC27
Synonyms:APC3, SNB1
Ordered Locus Names:YBL084C
ORF Names:YBL0718
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL084C
SGDiS000000180 CDC27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi267S → A: Abolishes phosphorylation; when associated with A-304; A-328; A-351 and A-397. 1 Publication1
Mutagenesisi304T → A: Abolishes phosphorylation; when associated with A-267; A-304; A-351 and A-397. 1 Publication1
Mutagenesisi328S → A: Abolishes phosphorylation; when associated with A-267; A-304; A-328 and A-397. 1 Publication1
Mutagenesisi351T → A: Abolishes phosphorylation; when associated with A-267; A-304; A-328 and A-304. 1 Publication1
Mutagenesisi397T → A: Abolishes phosphorylation; when associated with A-304; A-328; A-351 and A-397. 1 Publication1
Mutagenesisi613G → D in CDC27-633; G2/M cell cycle arrest at 35 degrees Celsius. 1 Publication1
Mutagenesisi614L → GL: Abolishes interaction with CDC23. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062741 – 758Anaphase-promoting complex subunit CDC27Add BLAST758

Post-translational modificationi

Phosphorylated by CDC28, which is required for the early mitotic activity of the APC/C in its CDC20-bound form.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38042
PaxDbiP38042
PRIDEiP38042

PTM databases

iPTMnetiP38042

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32620, 403 interactors
ComplexPortaliCPX-756 Anaphase-Promoting Complex variant 1
CPX-760 Anaphase-Promoting Complex variant 2
CPX-761 Anaphase-Promoting Complex variant 3
CPX-762 Anaphase-Promoting Complex variant 4
DIPiDIP-796N
ELMiP38042
IntActiP38042, 43 interactors
MINTiP38042
STRINGi4932.YBL084C

Structurei

3D structure databases

ProteinModelPortaliP38042
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati154 – 187TPR 1Add BLAST34
Repeati472 – 505TPR 2Add BLAST34
Repeati540 – 573TPR 3Add BLAST34
Repeati574 – 607TPR 4Add BLAST34
Repeati608 – 641TPR 5Add BLAST34
Repeati642 – 675TPR 6Add BLAST34
Repeati676 – 709TPR 7Add BLAST34
Repeati710 – 743TPR 8Add BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi365 – 388Poly-AsnAdd BLAST24

Sequence similaritiesi

Belongs to the APC3/CDC27 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00550000074887
HOGENOMiHOG000214050
InParanoidiP38042
KOiK03350
OMAiCCCGVAL
OrthoDBiEOG092C176G

Family and domain databases

Gene3Di1.25.40.10, 4 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 2 hits
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 8 hits
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50005 TPR, 8 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P38042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVNPELAPF TLSRGIPSFD DQALSTIIQL QDCIQQAIQQ LNYSTAEFLA
60 70 80 90 100
ELLYAECSIL DKSSVYWSDA VYLYALSLFL NKSYHTAFQI SKEFKEYHLG
110 120 130 140 150
IAYIFGRCAL QLSQGVNEAI LTLLSIINVF SSNSSNTRIN MVLNSNLVHI
160 170 180 190 200
PDLATLNCLL GNLYMKLDHS KEGAFYHSEA LAINPYLWES YEAICKMRAT
210 220 230 240 250
VDLKRVFFDI AGKKSNSHNN NAASSFPSTS LSHFEPRSQP SLYSKTNKNG
260 270 280 290 300
NNNINNNVNT LFQSSNSPPS TSASSFSSIQ HFSRSQQQQA NTSIRTCQNK
310 320 330 340 350
NTQTPKNPAI NSKTSSALPN NISMNLVSPS SKQPTISSLA KVYNRNKLLT
360 370 380 390 400
TPPSKLLNND RNHQNNNNNN NNNNNNNNNN NNNNNNNNII NKTTFKTPRN
410 420 430 440 450
LYSSTGRLTT SKKNPRSLII SNSILTSDYS ITLPEIMYNF ALILRSSSQY
460 470 480 490 500
NSFKAIRLFE SQIPSHIKDT MPWCLVQLGK LHFEIINYDM SLKYFNRLKD
510 520 530 540 550
LQPARVKDME IFSTLLWHLH DKVKSSNLAN GLMDTMPNKP ETWCCIGNLL
560 570 580 590 600
SLQKDHDAAI KAFEKATQLD PNFAYAYTLQ GHEHSSNDSS DSAKTCYRKA
610 620 630 640 650
LACDPQHYNA YYGLGTSAMK LGQYEEALLY FEKARSINPV NVVLICCCGG
660 670 680 690 700
SLEKLGYKEK ALQYYELACH LQPTSSLSKY KMGQLLYSMT RYNVALQTFE
710 720 730 740 750
ELVKLVPDDA TAHYLLGQTY RIVGRKKDAI KELTVAMNLD PKGNQVIIDE

LQKCHMQE
Length:758
Mass (Da):85,396
Last modified:July 27, 2011 - v2
Checksum:iA1ECA067AF8784E3
GO

Sequence cautioni

The sequence CAA56022 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti430S → Q in CAA56022 (PubMed:7502586).Curated1
Sequence conflicti430S → Q in CAA84905 (PubMed:7813418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA Translation: CAA56022.1 Different initiation.
Z35845 Genomic DNA Translation: CAA84905.1
BK006936 Genomic DNA Translation: DAA07040.2
PIRiS45825
RefSeqiNP_009469.2, NM_001178324.2

Genome annotation databases

EnsemblFungiiYBL084C; YBL084C; YBL084C
GeneIDi852194
KEGGisce:YBL084C

Similar proteinsi

Entry informationi

Entry nameiCDC27_YEAST
AccessioniPrimary (citable) accession number: P38042
Secondary accession number(s): D6VPS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: July 18, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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