UniProtKB - P38033 (NIFS_BACSU)
Protein
Putative cysteine desulfurase NifS
Gene
nifS
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). Seems to be required for NAD biosynthesis (PubMed:8444804).By similarity1 Publication
Catalytic activityi
- EC:2.8.1.7By similarity
Cofactori
pyridoxal 5'-phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 177 | Pyridoxal phosphateBy similarity | 1 | |
Binding sitei | 235 | Pyridoxal phosphateBy similarity | 1 | |
Active sitei | 325 | Cysteine persulfide intermediateBy similarity | 1 | |
Metal bindingi | 325 | Iron-sulfur (2Fe-2S); via persulfide groupBy similarity | 1 |
GO - Molecular functioni
- cysteine desulfurase activity Source: UniProtKB-EC
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Ligand | Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | BSUB:BSU27880-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nifS Synonyms:iscS Ordered Locus Names:BSU27880 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene require nicotinic acid for growth.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000150286 | 1 – 395 | Putative cysteine desulfurase NifSAdd BLAST | 395 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 200 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
jPOSTi | P38033 |
PaxDbi | P38033 |
PRIDEi | P38033 |
Expressioni
Inductioni
Repressed in the presence of nicotinic acid.1 Publication
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 69 – 70 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 197 – 199 | Pyridoxal phosphate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.Curated
Phylogenomic databases
eggNOGi | COG1104, Bacteria |
InParanoidi | P38033 |
OMAi | CLQSANH |
PhylomeDBi | P38033 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR020578, Aminotrans_V_PyrdxlP_BS IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00595, AA_TRANSFER_CLASS_5, 1 hit |
i Sequence
Sequence statusi: Complete.
P38033-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIYLDYAATT PICEEALTVY QKLSMDMYGN ASSLHDAGGK AKHILEYCRE
60 70 80 90 100
KIANIIGGEA SGIYFTSGGT ESNFLAIQSL LNGLPKTKRH FITTAMEHQS
110 120 130 140 150
IHNCAAFLEQ QGYDVTVIEP NEYGLITEEI LLTHIRPETG LVSIQHANSE
160 170 180 190 200
TGIIQPIQHL SSYLHNKGIL LHCDAVQTFG KIPINTKNLG VDALSMSSHK
210 220 230 240 250
IHGPKGVGAV YIRPDVPWKP VYPLTTHEYG FRAGTVNVPG IGAFTAAAEL
260 270 280 290 300
IVSEMEKQIS RNEALRTYFL DQIRIRSLPV TLAADTSKAE CLPHIIGCFF
310 320 330 340 350
HSFEGQYVML ECNRSNICIS TGSACSAGYH GPSETMKALR KTEQEALQFI
360 370 380 390
RISFGRHTTA EQLEQLLHTF TVLWEQKKGE FDIDRRIKAN GRQQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98822 Genomic DNA Translation: AAA21613.1 AL009126 Genomic DNA Translation: CAB14748.1 |
PIRi | B47071 |
RefSeqi | NP_390666.1, NC_000964.3 WP_004398844.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14748; CAB14748; BSU27880 |
GeneIDi | 936637 |
KEGGi | bsu:BSU27880 |
PATRICi | fig|224308.179.peg.3029 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98822 Genomic DNA Translation: AAA21613.1 AL009126 Genomic DNA Translation: CAB14748.1 |
PIRi | B47071 |
RefSeqi | NP_390666.1, NC_000964.3 WP_004398844.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P38033 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU27880 |
Proteomic databases
jPOSTi | P38033 |
PaxDbi | P38033 |
PRIDEi | P38033 |
Genome annotation databases
EnsemblBacteriai | CAB14748; CAB14748; BSU27880 |
GeneIDi | 936637 |
KEGGi | bsu:BSU27880 |
PATRICi | fig|224308.179.peg.3029 |
Phylogenomic databases
eggNOGi | COG1104, Bacteria |
InParanoidi | P38033 |
OMAi | CLQSANH |
PhylomeDBi | P38033 |
Enzyme and pathway databases
BioCyci | BSUB:BSU27880-MONOMER |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR020578, Aminotrans_V_PyrdxlP_BS IPR016454, Cysteine_dSase IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00266, Aminotran_5, 1 hit |
PIRSFi | PIRSF005572, NifS, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00595, AA_TRANSFER_CLASS_5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NIFS_BACSU | |
Accessioni | P38033Primary (citable) accession number: P38033 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families