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UniProtKB - P37957 (ESTA_BACSU)
Protein
Lipase EstA
Gene
estA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Active toward triacylglycerides with a preference for esters with C8:0 acyl groups; barely active on C18:1 or C18:4 substrates. Active against p-nitrophenylesters with fatty acid chain lengths from C6 to C18.
1 Publication2 PublicationsCatalytic activityi
- EC:3.1.1.32 Publications
Activity regulationi
Strongly inhibited when incubated with the serine reagent phenylmethylsulfonyl fluoride. Activated by the addition of calcium to the reaction mixture. When calcium was incubated with the lipase but not added to the reaction mixture, its effect is lower but still observable. Magnesium, manganese and strontium are not able to replace calcium with full retention of activity.1 Publication
pH dependencei
Optimum pH is 10 (PubMed:11029590). The activity decreases strongly above pH 10.5 or below pH 6.5. The enzyme is remarkably stable at alkaline pH, showing maximum stability at pH 12 and retaining more than 65% of its activity after 24 hours at pH 13 (PubMed:8396026).2 Publications
Temperature dependencei
Optimum temperature is 35 degrees Celsius. Stable for at least 30 minutes at 40 degrees Celsius. Virtually no activity remains after 30 minutes at 55 degrees Celsius.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 108 | Nucleophile3 Publications | 1 | |
Active sitei | 164 | Charge relay system3 Publications | 1 | |
Active sitei | 187 | Charge relay system3 Publications | 1 |
GO - Molecular functioni
- lipase activity Source: GO_Central
- triglyceride lipase activity Source: CACAO
GO - Biological processi
- lipid catabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
BioCyci | BSUB:BSU02700-MONOMER |
SABIO-RKi | P37957 |
Protein family/group databases
ESTHERi | bacsu-lip, Lipase_2 |
Names & Taxonomyi
Protein namesi | Recommended name: Lipase EstAAlternative name(s): Lipase A (EC:3.1.1.32 Publications) Triacylglycerol lipase |
Gene namesi | Name:estA Synonyms:lip1 Publication, lipA1 Publication Ordered Locus Names:BSU02700 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Chemistry databases
DrugBanki | DB08475, [(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl hydrogen hex-5-enylphosphonate DB08548, [(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl hydrogen hex-5-enylphosphonate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | 1 PublicationAdd BLAST | 31 | |
ChainiPRO_0000017819 | 32 – 212 | Lipase EstAAdd BLAST | 181 |
Proteomic databases
PaxDbi | P37957 |
Expressioni
Inductioni
Maximally expressed in late exponential growth phase. Expression decreases rapidly in the stationary phase. Expressed in both rich and minimal media with glucose as carbon source (at protein level).2 Publications
Interactioni
Subunit structurei
Monomer.
2 PublicationsProtein-protein interaction databases
STRINGi | 224308.BSU02700 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P37957 |
BMRBi | P37957 |
SMRi | P37957 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37957 |
Family & Domainsi
Sequence similaritiesi
Belongs to the AB hydrolase superfamily.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1075, Bacteria |
InParanoidi | P37957 |
OMAi | MIVMNYL |
PhylomeDBi | P37957 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002918, Lipase_EstA/Esterase_EstB |
PANTHERi | PTHR32015, PTHR32015, 1 hit |
Pfami | View protein in Pfam PF01674, Lipase_2, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P37957-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFVKRRIIA LVTILMLSVT SLFALQPSAK AAEHNPVVMV HGIGGASFNF
60 70 80 90 100
AGIKSYLVSQ GWSRDKLYAV DFWDKTGTNY NNGPVLSRFV QKVLDETGAK
110 120 130 140 150
KVDIVAHSMG GANTLYYIKN LDGGNKVANV VTLGGANRLT TGKALPGTDP
160 170 180 190 200
NQKILYTSIY SSADMIVMNY LSRLDGARNV QIHGVGHIGL LYSSQVNSLI
210
KEGLNGGGQN TN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 | A → V in ABB54395 (Ref. 3) Curated | 1 | |
Sequence conflicti | 51 | A → G in ABB54395 (Ref. 3) Curated | 1 | |
Sequence conflicti | 60 | Q → H in ABB54395 (Ref. 3) Curated | 1 | |
Sequence conflicti | 133 | L → V in AAA22574 (PubMed:1320940).Curated | 1 | |
Sequence conflicti | 133 | L → V in ABB54395 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74010 Genomic DNA Translation: AAA22574.1 AB000617 Genomic DNA Translation: BAA22231.1 DQ250714 Genomic DNA Translation: ABB54395.1 AL009126 Genomic DNA Translation: CAB12064.1 |
PIRi | S23934 |
RefSeqi | NP_388152.1, NC_000964.3 WP_003246250.1, NZ_JNCM01000030.1 |
Genome annotation databases
EnsemblBacteriai | CAB12064; CAB12064; BSU_02700 |
GeneIDi | 938389 |
KEGGi | bsu:BSU02700 |
PATRICi | fig|224308.179.peg.280 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74010 Genomic DNA Translation: AAA22574.1 AB000617 Genomic DNA Translation: BAA22231.1 DQ250714 Genomic DNA Translation: ABB54395.1 AL009126 Genomic DNA Translation: CAB12064.1 |
PIRi | S23934 |
RefSeqi | NP_388152.1, NC_000964.3 WP_003246250.1, NZ_JNCM01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1I6W | X-ray | 1.50 | A/B | 32-212 | [»] | |
1ISP | X-ray | 1.30 | A | 32-212 | [»] | |
1R4Z | X-ray | 1.80 | A/B | 32-212 | [»] | |
1R50 | X-ray | 1.45 | A/B | 32-212 | [»] | |
1T2N | X-ray | 1.80 | A | 32-212 | [»] | |
1T4M | X-ray | 2.00 | A | 32-212 | [»] | |
2QXT | X-ray | 2.00 | A/B | 34-212 | [»] | |
2QXU | X-ray | 1.90 | A/B/C/D/E/F/G/H | 34-212 | [»] | |
3D2A | X-ray | 1.73 | A | 32-212 | [»] | |
3D2B | X-ray | 1.95 | A/B | 32-212 | [»] | |
3D2C | X-ray | 2.18 | A/B/C/D/E/F/G/H/I/J/K/L | 32-212 | [»] | |
3QMM | X-ray | 1.89 | A/B | 32-212 | [»] | |
3QZU | X-ray | 1.85 | A/B | 32-212 | [»] | |
5CRI | X-ray | 1.63 | A/B | 32-212 | [»] | |
5CT4 | X-ray | 1.49 | A/B | 33-212 | [»] | |
5CT5 | X-ray | 1.75 | A/B | 33-212 | [»] | |
5CT6 | X-ray | 1.90 | A/B | 33-212 | [»] | |
5CT8 | X-ray | 1.29 | A | 33-212 | [»] | |
5CT9 | X-ray | 1.40 | A | 33-212 | [»] | |
5CTA | X-ray | 1.24 | A | 33-212 | [»] | |
5CUR | X-ray | 1.30 | A | 33-212 | [»] | |
AlphaFoldDBi | P37957 | |||||
BMRBi | P37957 | |||||
SMRi | P37957 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU02700 |
Chemistry databases
DrugBanki | DB08475, [(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl hydrogen hex-5-enylphosphonate DB08548, [(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl hydrogen hex-5-enylphosphonate |
Protein family/group databases
ESTHERi | bacsu-lip, Lipase_2 |
Proteomic databases
PaxDbi | P37957 |
Genome annotation databases
EnsemblBacteriai | CAB12064; CAB12064; BSU_02700 |
GeneIDi | 938389 |
KEGGi | bsu:BSU02700 |
PATRICi | fig|224308.179.peg.280 |
Phylogenomic databases
eggNOGi | COG1075, Bacteria |
InParanoidi | P37957 |
OMAi | MIVMNYL |
PhylomeDBi | P37957 |
Enzyme and pathway databases
BioCyci | BSUB:BSU02700-MONOMER |
SABIO-RKi | P37957 |
Miscellaneous databases
EvolutionaryTracei | P37957 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002918, Lipase_EstA/Esterase_EstB |
PANTHERi | PTHR32015, PTHR32015, 1 hit |
Pfami | View protein in Pfam PF01674, Lipase_2, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ESTA_BACSU | |
Accessioni | P37957Primary (citable) accession number: P37957 Secondary accession number(s): O34644, Q2XU59 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | May 30, 2000 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families