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UniProtKB - P37941 (ODBB_BACSU)
Protein
2-oxoisovalerate dehydrogenase subunit beta
Gene
bfmBAB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Catalytic activityi
- (R)-N6-lipoyl-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + 3-methyl-2-oxobutanoate + H+ = (R)-N6-(S8-2-methylpropanoyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + CO2EC:1.2.4.4
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 29 | Thiamine pyrophosphateBy similarity | 1 | |
Binding sitei | 82 | Thiamine pyrophosphateBy similarity | 1 | |
Active sitei | 129 | Proton acceptorBy similarity | 1 | |
Binding sitei | 129 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity Source: UniProtKB-EC
GO - Biological processi
- branched-chain amino acid catabolic process Source: GO_Central
- response to nutrient Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Thiamine pyrophosphate |
Enzyme and pathway databases
BioCyci | BSUB:BSU24040-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 2-oxoisovalerate dehydrogenase subunit beta (EC:1.2.4.4)Alternative name(s): Branched-chain alpha-keto acid dehydrogenase E1 component beta chain Short name: BCKDH E1-beta |
Gene namesi | Name:bfmBAB Synonyms:bfmB1b Ordered Locus Names:BSU24040 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162250 | 1 – 327 | 2-oxoisovalerate dehydrogenase subunit betaAdd BLAST | 327 |
Proteomic databases
jPOSTi | P37941 |
PaxDbi | P37941 |
PRIDEi | P37941 |
Interactioni
Subunit structurei
Heterotetramer of two alpha and two beta chains. Directly associated with ODBA in the E1 complex.
Protein-protein interaction databases
IntActi | P37941, 2 interactors |
STRINGi | 224308.BSU24040 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 58 – 60 | Thiamine pyrophosphate bindingBy similarity | 3 | |
Regioni | 83 – 86 | Substrate bindingBy similarity | 4 | |
Regioni | 86 – 89 | Thiamine pyrophosphate bindingBy similarity | 4 |
Phylogenomic databases
eggNOGi | COG0022, Bacteria |
InParanoidi | P37941 |
OMAi | SEAYYMA |
PhylomeDBi | P37941 |
Family and domain databases
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR033248, Transketolase_C |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit SSF52922, SSF52922, 1 hit |
i Sequence
Sequence statusi: Complete.
P37941-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVMSYIDAI NLAMKEEMER DSRVFVLGED VGRKGGVFKA TAGLYEQFGE
60 70 80 90 100
ERVMDTPLAE SAIAGVGIGA AMYGMRPIAE MQFADFIMPA VNQIISEAAK
110 120 130 140 150
IRYRSNNDWS CPIVVRAPYG GGVHGALYHS QSVEAIFANQ PGLKIVMPST
160 170 180 190 200
PYDAKGLLKA AVRDEDPVLF FEHKRAYRLI KGEVPADDYV LPIGKADVKR
210 220 230 240 250
EGDDITVITY GLCVHFALQA AERLEKDGIS AHVVDLRTVY PLDKEAIIEA
260 270 280 290 300
ASKTGKVLLV TEDTKEGSIM SEVAAIISEH CLFDLDAPIK RLAGPDIPAM
310 320
PYAPTMEKYF MVNPDKVEAA MRELAEF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97391 Genomic DNA Translation: AAA22279.1 D84432 Genomic DNA Translation: BAA12599.1 AL009126 Genomic DNA Translation: CAB14335.1 |
PIRi | D69593 |
RefSeqi | NP_390284.1, NC_000964.3 WP_004398638.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14335; CAB14335; BSU_24040 |
GeneIDi | 938672 |
KEGGi | bsu:BSU24040 |
PATRICi | fig|224308.179.peg.2618 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97391 Genomic DNA Translation: AAA22279.1 D84432 Genomic DNA Translation: BAA12599.1 AL009126 Genomic DNA Translation: CAB14335.1 |
PIRi | D69593 |
RefSeqi | NP_390284.1, NC_000964.3 WP_004398638.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | P37941 |
SMRi | P37941 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P37941, 2 interactors |
STRINGi | 224308.BSU24040 |
Proteomic databases
jPOSTi | P37941 |
PaxDbi | P37941 |
PRIDEi | P37941 |
Genome annotation databases
EnsemblBacteriai | CAB14335; CAB14335; BSU_24040 |
GeneIDi | 938672 |
KEGGi | bsu:BSU24040 |
PATRICi | fig|224308.179.peg.2618 |
Phylogenomic databases
eggNOGi | COG0022, Bacteria |
InParanoidi | P37941 |
OMAi | SEAYYMA |
PhylomeDBi | P37941 |
Enzyme and pathway databases
BioCyci | BSUB:BSU24040-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR033248, Transketolase_C |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit SSF52922, SSF52922, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ODBB_BACSU | |
Accessioni | P37941Primary (citable) accession number: P37941 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |