UniProtKB - P37794 (CHBG_ECOLI)
Protein
Chitooligosaccharide deacetylase ChbG
Gene
chbG
Organism
Escherichia coli (strain K12)
Status
Functioni
ChbG is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Deacetylation of chitobiose-6-P and chitotriose-6-P is necessary for both the activation of the chb promoter by the regulatory protein ChbR and the hydrolysis of phosphorylated beta-glucosides by the phospho-beta-glucosidase ChbF. Catalyzes the removal of only one acetyl group from chitobiose-6-P to yield monoacetylchitobiose-6-P, the inducer of ChbR and the substrate of ChbF. It can also use chitobiose and chitotriose as substrates.1 Publication
Caution
Was originally (PubMed:8121401 and PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.Curated
Catalytic activityi
- H2O + N,N'-diacetylchitobiose = acetate + N-acetyl-β-D-glucosaminyl-(1→4)-D-glucosamine1 PublicationEC:3.5.1.1051 Publication
Cofactori
Mg2+By similarity
: chitin degradation Pathwayi
This protein is involved in the pathway chitin degradation, which is part of Glycan degradation.1 PublicationView all proteins of this organism that are known to be involved in the pathway chitin degradation and in Glycan degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 61 | Magnesium; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 125 | Magnesium; via pros nitrogenBy similarity | 1 |
GO - Molecular functioni
- chitin disaccharide deacetylase activity Source: EcoCyc
- deacetylase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- chitin catabolic process Source: UniProtKB-UniPathway
- diacetylchitobiose catabolic process Source: EcoCyc
- polysaccharide catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase |
Biological process | Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12198-MONOMER MetaCyc:EG12198-MONOMER |
UniPathwayi | UPA00349 |
Names & Taxonomyi
Protein namesi | Recommended name: Chitooligosaccharide deacetylase ChbG1 Publication (EC:3.5.1.1051 Publication)Short name: COD1 Publication Alternative name(s): Chitin disaccharide deacetylase1 Publication Chitobiose deacetylase1 Publication Chitobiose-6P deacetylase1 Publication Chitotriose deacetylase1 Publication Chitotriose-6P deacetylase1 Publication |
Gene namesi | Name:chbG1 Publication Synonyms:ydjC Ordered Locus Names:b1733, JW1722 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene are unable to grow on chitobiose and chitotriose.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 11 | D → A: Unable to induce CnbR and to grow on chitobiose. 1 Publication | 1 | |
Mutagenesisi | 61 | H → A: Unable to induce CnbR and to grow on chitobiose. 1 Publication | 1 | |
Mutagenesisi | 125 | H → A: Unable to induce CnbR and to grow on chitobiose. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051589 | 1 – 249 | Chitooligosaccharide deacetylase ChbGAdd BLAST | 249 |
Proteomic databases
jPOSTi | P37794 |
PaxDbi | P37794 |
PRIDEi | P37794 |
Expressioni
Inductioni
By N,N'-diacetylchitobiose.1 Publication
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4260305, 16 interactors |
IntActi | P37794, 4 interactors |
STRINGi | 511145.b1733 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG3394, Bacteria |
HOGENOMi | CLU_064244_4_1_6 |
InParanoidi | P37794 |
PhylomeDBi | P37794 |
Family and domain databases
CDDi | cd10803, YdjC_EF3048_like, 1 hit |
HAMAPi | MF_01246, COD, 1 hit |
InterProi | View protein in InterPro IPR022948, COD_ChbG_bac IPR011330, Glyco_hydro/deAcase_b/a-brl IPR006879, YdjC-like |
PANTHERi | PTHR31609, PTHR31609, 2 hits |
Pfami | View protein in Pfam PF04794, YdjC, 1 hit |
SUPFAMi | SSF88713, SSF88713, 1 hit |
i Sequence
Sequence statusi: Complete.
P37794-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERLLIVNAD DFGLSKGQNY GIIEACRNGI VTSTTALVNG QAIDHAVQLS
60 70 80 90 100
RDEPSLAIGM HFVLTMGKPL TAMPGLTRDG VLGKWIWQLA EEDALPLEEI
110 120 130 140 150
TQELVSQYLR FIELFGRKPT HLDSHHHVHM FPQIFPIVAR FAAEQGIALR
160 170 180 190 200
ADRQMAFDLP VNLRTTQGFS SAFYGEEISE SLFLQVLDDA GHRGDRSLEV
210 220 230 240
MCHPAFIDNT IRQSAYCFPR LTELDVLTSA SLKGAIAQRG YRLGSYRDV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | I → L in CAA47260 (PubMed:8121401).Curated | 1 | |
Sequence conflicti | 6 | I → L in X52890 (PubMed:2179047).Curated | 1 | |
Sequence conflicti | 16 – 40 | KGQNY…ALVNG → QRTELRHYRGLSQWDCHCRR RHCEW in CAA47260 (PubMed:8121401).CuratedAdd BLAST | 25 | |
Sequence conflicti | 16 – 40 | KGQNY…ALVNG → QRTELRHYRGLSQWDCHCRR RHCEW in X52890 (PubMed:2179047).CuratedAdd BLAST | 25 | |
Sequence conflicti | 48 | Q → H in CAA47260 (PubMed:8121401).Curated | 1 | |
Sequence conflicti | 48 | Q → H in X52890 (PubMed:2179047).Curated | 1 | |
Sequence conflicti | 51 | R → C in CAA47260 (PubMed:8121401).Curated | 1 | |
Sequence conflicti | 51 | R → C in X52890 (PubMed:2179047).Curated | 1 | |
Sequence conflicti | 55 | S → I in CAA47260 (PubMed:8121401).Curated | 1 | |
Sequence conflicti | 55 | S → I in X52890 (PubMed:2179047).Curated | 1 | |
Sequence conflicti | 65 | T → I in CAA47260 (PubMed:8121401).Curated | 1 | |
Sequence conflicti | 65 | T → I in X52890 (PubMed:2179047).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66725 Genomic DNA Translation: CAA47260.1 X66725 Genomic DNA Translation: CAA47261.1 U00096 Genomic DNA Translation: AAC74803.1 AP009048 Genomic DNA Translation: BAA15514.1 X52890 Genomic DNA No translation available. M55161 Genomic DNA No translation available. |
PIRi | E64932 |
RefSeqi | NP_416247.1, NC_000913.3 WP_000440471.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74803; AAC74803; b1733 BAA15514; BAA15514; BAA15514 |
GeneIDi | 57731129 946231 |
KEGGi | ecj:JW1722 eco:b1733 |
PATRICi | fig|1411691.4.peg.523 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66725 Genomic DNA Translation: CAA47260.1 X66725 Genomic DNA Translation: CAA47261.1 U00096 Genomic DNA Translation: AAC74803.1 AP009048 Genomic DNA Translation: BAA15514.1 X52890 Genomic DNA No translation available. M55161 Genomic DNA No translation available. |
PIRi | E64932 |
RefSeqi | NP_416247.1, NC_000913.3 WP_000440471.1, NZ_SSZK01000001.1 |
3D structure databases
SMRi | P37794 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260305, 16 interactors |
IntActi | P37794, 4 interactors |
STRINGi | 511145.b1733 |
Proteomic databases
jPOSTi | P37794 |
PaxDbi | P37794 |
PRIDEi | P37794 |
Genome annotation databases
EnsemblBacteriai | AAC74803; AAC74803; b1733 BAA15514; BAA15514; BAA15514 |
GeneIDi | 57731129 946231 |
KEGGi | ecj:JW1722 eco:b1733 |
PATRICi | fig|1411691.4.peg.523 |
Organism-specific databases
EchoBASEi | EB2115 |
Phylogenomic databases
eggNOGi | COG3394, Bacteria |
HOGENOMi | CLU_064244_4_1_6 |
InParanoidi | P37794 |
PhylomeDBi | P37794 |
Enzyme and pathway databases
UniPathwayi | UPA00349 |
BioCyci | EcoCyc:EG12198-MONOMER MetaCyc:EG12198-MONOMER |
Miscellaneous databases
PROi | PR:P37794 |
Family and domain databases
CDDi | cd10803, YdjC_EF3048_like, 1 hit |
HAMAPi | MF_01246, COD, 1 hit |
InterProi | View protein in InterPro IPR022948, COD_ChbG_bac IPR011330, Glyco_hydro/deAcase_b/a-brl IPR006879, YdjC-like |
PANTHERi | PTHR31609, PTHR31609, 2 hits |
Pfami | View protein in Pfam PF04794, YdjC, 1 hit |
SUPFAMi | SSF88713, SSF88713, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHBG_ECOLI | |
Accessioni | P37794Primary (citable) accession number: P37794 Secondary accession number(s): P77435 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families