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Protein

Chitooligosaccharide deacetylase ChbG

Gene

chbG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ChbG is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Deacetylation of chitobiose-6-P and chitotriose-6-P is necessary for both the activation of the chb promoter by the regulatory protein ChbR and the hydrolysis of phosphorylated beta-glucosides by the phospho-beta-glucosidase ChbF. Catalyzes the removal of only one acetyl group from chitobiose-6-P to yield monoacetylchitobiose-6-P, the inducer of ChbR and the substrate of ChbF. It can also use chitobiose and chitotriose as substrates.1 Publication

Caution

Was originally (PubMed:8121401 and PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.Curated

Catalytic activityi

N,N'-diacetylchitobiose + H2O = N-acetyl-beta-D-glucosaminyl-(1->4)-D-glucosamine + acetate.1 Publication
Diacetylchitobiose-6-phosphate + H2O = N-monoacetylchitobiose-6-phosphate + acetate.1 Publication

Cofactori

Mg2+By similarity

Pathwayi: chitin degradation

This protein is involved in the pathway chitin degradation, which is part of Glycan degradation.1 Publication
View all proteins of this organism that are known to be involved in the pathway chitin degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Magnesium; via tele nitrogenBy similarity1
Metal bindingi125Magnesium; via pros nitrogenBy similarity1

GO - Molecular functioni

  • chitin disaccharide deacetylase activity Source: EcoCyc
  • deacetylase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chitin catabolic process Source: UniProtKB-UniPathway
  • diacetylchitobiose catabolic process Source: EcoCyc
  • polysaccharide catabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12198-MONOMER
MetaCyc:EG12198-MONOMER
UniPathwayiUPA00349

Names & Taxonomyi

Protein namesi
Recommended name:
Chitooligosaccharide deacetylase ChbG1 Publication (EC:3.5.1.1051 Publication)
Short name:
COD1 Publication
Alternative name(s):
Chitin disaccharide deacetylase1 Publication
Chitobiose deacetylase1 Publication
Chitobiose-6P deacetylase1 Publication
Chitotriose deacetylase1 Publication
Chitotriose-6P deacetylase1 Publication
Gene namesi
Name:chbG1 Publication
Synonyms:ydjC
Ordered Locus Names:b1733, JW1722
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12198 chbG

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are unable to grow on chitobiose and chitotriose.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi11D → A: Unable to induce CnbR and to grow on chitobiose. 1 Publication1
Mutagenesisi61H → A: Unable to induce CnbR and to grow on chitobiose. 1 Publication1
Mutagenesisi125H → A: Unable to induce CnbR and to grow on chitobiose. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000515891 – 249Chitooligosaccharide deacetylase ChbGAdd BLAST249

Proteomic databases

PaxDbiP37794
PRIDEiP37794

Expressioni

Inductioni

By N,N'-diacetylchitobiose.1 Publication

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi4260305, 16 interactors
IntActiP37794, 4 interactors
STRINGi316385.ECDH10B_1871

Structurei

3D structure databases

ProteinModelPortaliP37794
SMRiP37794
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the YdjC deacetylase family. ChbG subfamily.Curated

Phylogenomic databases

eggNOGiENOG410901P Bacteria
COG3394 LUCA
HOGENOMiHOG000225034
InParanoidiP37794
KOiK03478
OMAiEPTHIDS
PhylomeDBiP37794

Family and domain databases

CDDicd10803 YdjC_EF3048_like, 1 hit
HAMAPiMF_01246 COD, 1 hit
InterProiView protein in InterPro
IPR022948 COD_ChbG_bac
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR006879 YdjC-like
PANTHERiPTHR31609 PTHR31609, 2 hits
PfamiView protein in Pfam
PF04794 YdjC, 1 hit
SUPFAMiSSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

P37794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERLLIVNAD DFGLSKGQNY GIIEACRNGI VTSTTALVNG QAIDHAVQLS
60 70 80 90 100
RDEPSLAIGM HFVLTMGKPL TAMPGLTRDG VLGKWIWQLA EEDALPLEEI
110 120 130 140 150
TQELVSQYLR FIELFGRKPT HLDSHHHVHM FPQIFPIVAR FAAEQGIALR
160 170 180 190 200
ADRQMAFDLP VNLRTTQGFS SAFYGEEISE SLFLQVLDDA GHRGDRSLEV
210 220 230 240
MCHPAFIDNT IRQSAYCFPR LTELDVLTSA SLKGAIAQRG YRLGSYRDV
Length:249
Mass (Da):27,774
Last modified:July 15, 1998 - v2
Checksum:i1D4747904C974F11
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6I → L in CAA47260 (PubMed:8121401).Curated1
Sequence conflicti6I → L in X52890 (PubMed:2179047).Curated1
Sequence conflicti16 – 40KGQNY…ALVNG → QRTELRHYRGLSQWDCHCRR RHCEW in CAA47260 (PubMed:8121401).CuratedAdd BLAST25
Sequence conflicti16 – 40KGQNY…ALVNG → QRTELRHYRGLSQWDCHCRR RHCEW in X52890 (PubMed:2179047).CuratedAdd BLAST25
Sequence conflicti48Q → H in CAA47260 (PubMed:8121401).Curated1
Sequence conflicti48Q → H in X52890 (PubMed:2179047).Curated1
Sequence conflicti51R → C in CAA47260 (PubMed:8121401).Curated1
Sequence conflicti51R → C in X52890 (PubMed:2179047).Curated1
Sequence conflicti55S → I in CAA47260 (PubMed:8121401).Curated1
Sequence conflicti55S → I in X52890 (PubMed:2179047).Curated1
Sequence conflicti65T → I in CAA47260 (PubMed:8121401).Curated1
Sequence conflicti65T → I in X52890 (PubMed:2179047).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66725 Genomic DNA Translation: CAA47260.1
X66725 Genomic DNA Translation: CAA47261.1
U00096 Genomic DNA Translation: AAC74803.1
AP009048 Genomic DNA Translation: BAA15514.1
X52890 Genomic DNA No translation available.
M55161 Genomic DNA No translation available.
PIRiE64932
RefSeqiNP_416247.1, NC_000913.3
WP_000440471.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74803; AAC74803; b1733
BAA15514; BAA15514; BAA15514
GeneIDi946231
KEGGiecj:JW1722
eco:b1733
PATRICifig|1411691.4.peg.523

Similar proteinsi

Entry informationi

Entry nameiCHBG_ECOLI
AccessioniPrimary (citable) accession number: P37794
Secondary accession number(s): P77435
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 15, 1998
Last modified: March 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

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