UniProtKB - P37774 (TCYN_ECOLI)
Protein
L-cystine transport system ATP-binding protein TcyN
Gene
tcyN
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex TcyJLN involved in L-cystine import (PubMed:25139244, PubMed:20351115, PubMed:25837721, PubMed:26350134). This high affinity cystine transporter is involved in resistance to oxidative stress by forming a L-cysteine/L-cystine shuttle system with the EamA transporter, which exports L-cysteine as reducing equivalents to the periplasm to prevent the cells from oxidative stress. Exported L-cysteine can reduce the periplasmic hydrogen peroxide to water, and then generated L-cystine is imported back into the cytoplasm via the TcyJLN complex (PubMed:20351115, PubMed:25837721). Functions at low cystine concentrations (PubMed:26350134). The system can also transport L-cysteine, diaminopimelic acid (DAP), djenkolate, lanthionine, D-cystine, homocystine, and it mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys) (PubMed:25139244, PubMed:26350134). Could also facilitate threonine efflux (PubMed:28911185). Responsible for energy coupling to the transport system (Probable).Curated5 Publications
Catalytic activityi
Activity regulationi
The TcyJLN system is inhibited by L-cystine, L-cysteine, DL-2,6-diaminopimelic acid and L-cystathionine, and is stimulated by D-cysteine.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 43 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATPase-coupled amino acid transmembrane transporter activity Source: InterPro
- ATP binding Source: EcoCyc
- L-cystine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- L-cystine transport Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Biological process | Amino-acid transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12347-MONOMER MetaCyc:EG12347-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.3.10, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: L-cystine transport system ATP-binding protein TcyNCurated (EC:7.4.2.121 Publication) |
Gene namesi | Name:tcyN1 Publication Synonyms:yecC Ordered Locus Names:b1917, JW1902 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Peripheral membrane protein Curated
Plasma Membrane
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Disruption of the gene decreases uptake of cystine.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093166 | 1 – 250 | L-cystine transport system ATP-binding protein TcyNAdd BLAST | 250 |
Proteomic databases
jPOSTi | P37774 |
PaxDbi | P37774 |
PRIDEi | P37774 |
Expressioni
Inductioni
Expression is induced by H2O2.1 Publication
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL) and a solute-binding protein (TcyJ).
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4260366, 18 interactors |
ComplexPortali | CPX-4406, L-cystine ABC transporter complex |
DIPi | DIP-11822N |
IntActi | P37774, 5 interactors |
STRINGi | 511145.b1917 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 244 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 241 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily.Curated
Phylogenomic databases
eggNOGi | COG1126, Bacteria |
HOGENOMi | CLU_000604_1_22_6 |
InParanoidi | P37774 |
PhylomeDBi | P37774 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR030679, ABC_ATPase_HisP-typ IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
PIRSFi | PIRSF039085, ABC_ATPase_HisP, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P37774-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAIEVKNLV KKFHGQTVLH GIDLEVKPGE VVAIIGPSGS GKTTLLRSIN
60 70 80 90 100
LLEQPEAGTI TVGDITIDTA RSLSQQKSLI RQLRQHVGFV FQNFNLFPHR
110 120 130 140 150
TVLENIIEGP VIVKGEPKEE ATARARELLA KVGLAGKETS YPRRLSGGQQ
160 170 180 190 200
QRVAIARALA MRPEVILFDE PTSALDPELV GEVLNTIRQL AQEKRTMVIV
210 220 230 240 250
THEMSFARDV ADRAIFMDQG RIVEQGAAKA LFADPEQPRT RQFLEKFLLQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74984.1 AP009048 Genomic DNA Translation: BAA15737.1 X03691 Genomic DNA No translation available. |
PIRi | B64955 |
RefSeqi | NP_416427.1, NC_000913.3 WP_001272991.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74984; AAC74984; b1917 BAA15737; BAA15737; BAA15737 |
GeneIDi | 946422 |
KEGGi | ecj:JW1902 eco:b1917 |
PATRICi | fig|1411691.4.peg.332 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74984.1 AP009048 Genomic DNA Translation: BAA15737.1 X03691 Genomic DNA No translation available. |
PIRi | B64955 |
RefSeqi | NP_416427.1, NC_000913.3 WP_001272991.1, NZ_LN832404.1 |
3D structure databases
SMRi | P37774 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260366, 18 interactors |
ComplexPortali | CPX-4406, L-cystine ABC transporter complex |
DIPi | DIP-11822N |
IntActi | P37774, 5 interactors |
STRINGi | 511145.b1917 |
Protein family/group databases
TCDBi | 3.A.1.3.10, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P37774 |
PaxDbi | P37774 |
PRIDEi | P37774 |
Genome annotation databases
EnsemblBacteriai | AAC74984; AAC74984; b1917 BAA15737; BAA15737; BAA15737 |
GeneIDi | 946422 |
KEGGi | ecj:JW1902 eco:b1917 |
PATRICi | fig|1411691.4.peg.332 |
Organism-specific databases
EchoBASEi | EB2251 |
Phylogenomic databases
eggNOGi | COG1126, Bacteria |
HOGENOMi | CLU_000604_1_22_6 |
InParanoidi | P37774 |
PhylomeDBi | P37774 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12347-MONOMER MetaCyc:EG12347-MONOMER |
Miscellaneous databases
PROi | PR:P37774 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR030679, ABC_ATPase_HisP-typ IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
PIRSFi | PIRSF039085, ABC_ATPase_HisP, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCYN_ECOLI | |
Accessioni | P37774Primary (citable) accession number: P37774 Secondary accession number(s): P76314 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families