UniProtKB - P37773 (MPL_ECOLI)
Protein
UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase
Gene
mpl
Organism
Escherichia coli (strain K12)
Status
Functioni
Reutilizes the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramate. The enzyme can also use the tetrapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine or the pentapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioyl-D-alanyl-D-alanine in vivo and in vitro.2 Publications
Catalytic activityi
- ATP + L-alanyl-γ-D-glutamyl-meso-diaminoheptanedioate + UDP-N-acetyl-α-D-muramate = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminoheptanedioateUniRule annotation1 PublicationEC:6.3.2.45UniRule annotation1 Publication
Cofactori
Mg2+UniRule annotation1 Publication
Kineticsi
kcat is 290 min(-1).1 Publication
- KM=0.25 mM for UDP-N-acetyl-alpha-D-muramate1 Publication
- KM=0.19 mM for ATP1 Publication
- KM=0.1 mM for L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate1 Publication
- Vmax=5.8 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 8.4.1 Publication
: peptidoglycan recycling Pathwayi
This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 110 – 116 | ATPUniRule annotation | 7 |
GO - Molecular functioni
- acid-amino acid ligase activity Source: EcoCyc
- ATP binding Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-KW
- peptidoglycan turnover Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12440-MONOMER MetaCyc:EG12440-MONOMER |
BRENDAi | 6.3.2.B21, 2026 |
UniPathwayi | UPA00544 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligaseUniRule annotation (EC:6.3.2.45UniRule annotation1 Publication)Alternative name(s): Murein peptide ligaseUniRule annotation UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligaseUniRule annotation |
Gene namesi | Name:mpl1 PublicationUniRule annotation Synonyms:yjfG Ordered Locus Names:b4233, JW4192 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: EcoliWiki
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
Enzyme ligase activity totally absent. No effect on growth.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101708 | 1 – 457 | UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligaseAdd BLAST | 457 |
Proteomic databases
jPOSTi | P37773 |
PaxDbi | P37773 |
PRIDEi | P37773 |
Interactioni
Binary interactionsi
P37773
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-562735,EBI-562735 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259323, 197 interactors 853041, 3 interactors |
DIPi | DIP-10246N |
IntActi | P37773, 7 interactors |
STRINGi | 511145.b4233 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0773, Bacteria |
HOGENOMi | CLU_028104_0_1_6 |
InParanoidi | P37773 |
PhylomeDBi | P37773 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02020, Mpl, 1 hit |
InterProi | View protein in InterPro IPR005757, Mpl IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit |
TIGRFAMsi | TIGR01081, mpl, 1 hit |
i Sequence
Sequence statusi: Complete.
P37773-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIHILGICG TFMGGLAMLA RQLGHEVTGS DANVYPPMST LLEKQGIELI
60 70 80 90 100
QGYDASQLEP QPDLVIIGNA MTRGNPCVEA VLEKNIPYMS GPQWLHDFVL
110 120 130 140 150
RDRWVLAVAG THGKTTTAGM ATWILEQCGY KPGFVIGGVP GNFEVSAHLG
160 170 180 190 200
ESDFFVIEAD EYDCAFFDKR SKFVHYCPRT LILNNLEFDH ADIFDDLKAI
210 220 230 240 250
QKQFHHLVRI VPGQGRIIWP ENDINLKQTM AMGCWSEQEL VGEQGHWQAK
260 270 280 290 300
KLTTDASEWE VLLDGEKVGE VKWSLVGEHN MHNGLMAIAA ARHVGVAPAD
310 320 330 340 350
AANALGSFIN ARRRLELRGE ANGVTVYDDF AHHPTAILAT LAALRGKVGG
360 370 380 390 400
TARIIAVLEP RSNTMKMGIC KDDLAPSLGR ADEVFLLQPA HIPWQVAEVA
410 420 430 440 450
EACVQPAHWS GDVDTLADMV VKTAQPGDHI LVMSNGGFGG IHQKLLDGLA
KKAEAAQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97130.1 U00096 Genomic DNA Translation: AAC77190.1 AP009048 Genomic DNA Translation: BAE78233.1 X12545 mRNA No translation available. |
PIRi | S56459 |
RefSeqi | NP_418654.1, NC_000913.3 WP_001219813.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77190; AAC77190; b4233 BAE78233; BAE78233; BAE78233 |
GeneIDi | 948752 |
KEGGi | ecj:JW4192 eco:b4233 |
PATRICi | fig|1411691.4.peg.2469 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97130.1 U00096 Genomic DNA Translation: AAC77190.1 AP009048 Genomic DNA Translation: BAE78233.1 X12545 mRNA No translation available. |
PIRi | S56459 |
RefSeqi | NP_418654.1, NC_000913.3 WP_001219813.1, NZ_LN832404.1 |
3D structure databases
SMRi | P37773 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259323, 197 interactors 853041, 3 interactors |
DIPi | DIP-10246N |
IntActi | P37773, 7 interactors |
STRINGi | 511145.b4233 |
Proteomic databases
jPOSTi | P37773 |
PaxDbi | P37773 |
PRIDEi | P37773 |
Genome annotation databases
EnsemblBacteriai | AAC77190; AAC77190; b4233 BAE78233; BAE78233; BAE78233 |
GeneIDi | 948752 |
KEGGi | ecj:JW4192 eco:b4233 |
PATRICi | fig|1411691.4.peg.2469 |
Organism-specific databases
EchoBASEi | EB2335 |
Phylogenomic databases
eggNOGi | COG0773, Bacteria |
HOGENOMi | CLU_028104_0_1_6 |
InParanoidi | P37773 |
PhylomeDBi | P37773 |
Enzyme and pathway databases
UniPathwayi | UPA00544 |
BioCyci | EcoCyc:EG12440-MONOMER MetaCyc:EG12440-MONOMER |
BRENDAi | 6.3.2.B21, 2026 |
Miscellaneous databases
PROi | PR:P37773 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02020, Mpl, 1 hit |
InterProi | View protein in InterPro IPR005757, Mpl IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit |
TIGRFAMsi | TIGR01081, mpl, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPL_ECOLI | |
Accessioni | P37773Primary (citable) accession number: P37773 Secondary accession number(s): P76804, Q2M673 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | July 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families