UniProtKB - P37765 (RLUB_ECOLI)
Protein
Ribosomal large subunit pseudouridine synthase B
Gene
rluB
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA.1 Publication
Catalytic activityi
- EC:5.4.99.221 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 110 | NucleophileCurated | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
- rRNA pseudouridine synthase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Keywordsi
Molecular function | Isomerase, RNA-binding |
Biological process | rRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12433-MONOMER MetaCyc:EG12433-MONOMER |
BRENDAi | 5.4.99.22, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal large subunit pseudouridine synthase B (EC:5.4.99.22)Alternative name(s): 23S rRNA pseudouridine(2605) synthase rRNA pseudouridylate synthase B rRNA-uridine isomerase B |
Gene namesi | Name:rluB Synonyms:yciL Ordered Locus Names:b1269, JW1261 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 110 | D → N or T: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000099983 | 1 – 291 | Ribosomal large subunit pseudouridine synthase BAdd BLAST | 291 |
Proteomic databases
jPOSTi | P37765 |
PaxDbi | P37765 |
PRIDEi | P37765 |
Interactioni
Binary interactionsi
Hide detailsP37765
With | #Exp. | IntAct |
---|---|---|
rlmL [P75864] | 3 | EBI-561550,EBI-547718 |
rpsG [P02359] | 4 | EBI-561550,EBI-543074 |
srmB [P21507] | 6 | EBI-561550,EBI-546628 |
Protein-protein interaction databases
BioGRIDi | 4259574, 50 interactors 850207, 3 interactors |
DIPi | DIP-11585N |
IntActi | P37765, 79 interactors |
STRINGi | 511145.b1269 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37765 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 75 | S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST | 73 |
Sequence similaritiesi
Belongs to the pseudouridine synthase RsuA family.Curated
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_1_1_6 |
InParanoidi | P37765 |
PhylomeDBi | P37765 |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit PS50889, S4, 1 hit |
i Sequence
Sequence statusi: Complete.
P37765-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEKLQKVLA RAGHGSRREI ESIIEAGRVS VDGKIAKLGD RVEVTPGLKI
60 70 80 90 100
RIDGHLISVR ESAEQICRVL AYYKPEGELC TRNDPEGRPT VFDRLPKLRG
110 120 130 140 150
ARWIAVGRLD VNTCGLLLFT TDGELANRLM HPSREVEREY AVRVFGQVDD
160 170 180 190 200
AKLRDLSRGV QLEDGPAAFK TIKFSGGEGI NQWYNVTLTE GRNREVRRLW
210 220 230 240 250
EAVGVQVSRL IRVRYGDIPL PKGLPRGGWT ELDLAQTNYL RELVELPPET
260 270 280 290
SSKVAVEKDR RRMKANQIRR AVKRHSQVSG GRRSGGRNNN G
Sequence cautioni
The sequence M21528 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74351.1 AP009048 Genomic DNA Translation: BAA14806.1 U18111 Genomic DNA Translation: AAB59990.1 M21528 Genomic DNA No translation available. |
PIRi | H64874 |
RefSeqi | NP_415785.1, NC_000913.3 WP_001291217.1, NZ_SSZK01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC74351; AAC74351; b1269 BAA14806; BAA14806; BAA14806 |
GeneIDi | 58460473 945840 |
KEGGi | ecj:JW1261 eco:b1269 |
PATRICi | fig|1411691.4.peg.1015 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74351.1 AP009048 Genomic DNA Translation: BAA14806.1 U18111 Genomic DNA Translation: AAB59990.1 M21528 Genomic DNA No translation available. |
PIRi | H64874 |
RefSeqi | NP_415785.1, NC_000913.3 WP_001291217.1, NZ_SSZK01000031.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4LAB | X-ray | 2.50 | A | 1-251 | [»] | |
4LGT | X-ray | 1.30 | A/D | 1-251 | [»] | |
SMRi | P37765 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259574, 50 interactors 850207, 3 interactors |
DIPi | DIP-11585N |
IntActi | P37765, 79 interactors |
STRINGi | 511145.b1269 |
Proteomic databases
jPOSTi | P37765 |
PaxDbi | P37765 |
PRIDEi | P37765 |
Genome annotation databases
EnsemblBacteriai | AAC74351; AAC74351; b1269 BAA14806; BAA14806; BAA14806 |
GeneIDi | 58460473 945840 |
KEGGi | ecj:JW1261 eco:b1269 |
PATRICi | fig|1411691.4.peg.1015 |
Organism-specific databases
EchoBASEi | EB2329 |
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_1_1_6 |
InParanoidi | P37765 |
PhylomeDBi | P37765 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12433-MONOMER MetaCyc:EG12433-MONOMER |
BRENDAi | 5.4.99.22, 2026 |
Miscellaneous databases
PROi | PR:P37765 |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
Gene3Di | 3.10.290.10, 1 hit |
InterProi | View protein in InterPro IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit PS50889, S4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RLUB_ECOLI | |
Accessioni | P37765Primary (citable) accession number: P37765 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families