UniProtKB - P37760 (RMLD_ECOLI)
Protein
dTDP-4-dehydrorhamnose reductase
Gene
rfbD
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the biosynthesis of the dTDP-L-rhamnose which is an important component of lipopolysaccharide (LPS) (Probable). Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose (By similarity). RmlD uses NADH and NADPH nearly equally well (By similarity).By similarity1 Publication
Catalytic activityi
- EC:1.1.1.133By similarity
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity
: dTDP-L-rhamnose biosynthesis Pathwayi
This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.
Pathwayi: LPS O-antigen biosynthesis
This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 30 | NADBy similarity | 1 | |
Binding sitei | 102 | NADP; via carbonyl oxygenBy similarity | 1 | |
Sitei | 104 | Could provide a fine-tuning to achieve optimal pKa matching between active site and substrateBy similarity | 1 | |
Active sitei | 128 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 132 | NAD or NADPBy similarity | 1 | |
Binding sitei | 153 | Substrate; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 12 | NAD or NADPBy similarity | 3 | |
Nucleotide bindingi | 39 – 40 | NAD or NADPBy similarity | 2 | |
Nucleotide bindingi | 63 – 65 | NAD or NADPBy similarity | 3 |
GO - Molecular functioni
- dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- dTDP-rhamnose biosynthetic process Source: GO_Central
- extracellular polysaccharide biosynthetic process Source: UniProtKB
- lipopolysaccharide biosynthetic process Source: UniProtKB
- O antigen biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Carbohydrate metabolism, Lipopolysaccharide biosynthesis |
Ligand | Magnesium, Metal-binding, NAD, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:DTDPDEHYRHAMREDUCT-MONOMER |
UniPathwayi | UPA00124 UPA00281 |
Names & Taxonomyi
Protein namesi | Recommended name: dTDP-4-dehydrorhamnose reductaseBy similarity (EC:1.1.1.133By similarity)Alternative name(s): dTDP-4-keto-L-rhamnose reductaseBy similarity dTDP-6-deoxy-L-lyxo-4-hexulose reductaseBy similarity dTDP-6-deoxy-L-mannose dehydrogenaseBy similarity dTDP-L-rhamnose synthaseBy similarity |
Gene namesi | Name:rfbD1 Publication Synonyms:rmlD Ordered Locus Names:b2040, JW2025 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
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PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207984 | 1 – 299 | dTDP-4-dehydrorhamnose reductaseAdd BLAST | 299 |
Proteomic databases
jPOSTi | P37760 |
PaxDbi | P37760 |
PRIDEi | P37760 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 4261549, 180 interactors |
IntActi | P37760, 5 interactors |
STRINGi | 511145.b2040 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 104 – 105 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the dTDP-4-dehydrorhamnose reductase family.Curated
Phylogenomic databases
eggNOGi | COG1091, Bacteria |
HOGENOMi | CLU_045518_1_0_6 |
InParanoidi | P37760 |
PhylomeDBi | P37760 |
Family and domain databases
InterProi | View protein in InterPro IPR005913, dTDP_dehydrorham_reduct IPR036291, NAD(P)-bd_dom_sf IPR029903, RmlD-like-bd |
PANTHERi | PTHR10491, PTHR10491, 1 hit |
Pfami | View protein in Pfam PF04321, RmlD_sub_bind, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01214, rmlD, 1 hit |
i Sequence
Sequence statusi: Complete.
P37760-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNILLFGKTG QVGWELQRAL APLGNLIAFD VHSTDYCGDF SNPEGVAETV
60 70 80 90 100
RSIRPDIIVN AAAHTAVDKA ESEPEFAQLI NATSVEAIAK AANEVGAWVI
110 120 130 140 150
HYSTDYVFPG NGDMPWLETD ATAPLNVYGE TKLAGEKALQ EYCAKHLIFR
160 170 180 190 200
TSWVYAGKGN NFAKTMLRLA KEREELAVIN DQFGAPTGAE LLADCTAHAI
210 220 230 240 250
RVALNKPDVA GLYHLVASGT TTWYDYAALV FEEARKAGIP LALNKLNAVP
260 270 280 290
TTAYPTPARR PHNSRLNTEK FQQNFALVLP DWQVGVKRML NELFTTTAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09876 Genomic DNA Translation: AAB88399.1 U00096 Genomic DNA Translation: AAC75101.1 AP009048 Genomic DNA Translation: BAA15882.1 |
PIRi | G64969 |
RefSeqi | NP_416544.1, NC_000913.3 WP_001023610.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75101; AAC75101; b2040 BAA15882; BAA15882; BAA15882 |
GeneIDi | 947117 |
KEGGi | ecj:JW2025 eco:b2040 |
PATRICi | fig|511145.12.peg.2117 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09876 Genomic DNA Translation: AAB88399.1 U00096 Genomic DNA Translation: AAC75101.1 AP009048 Genomic DNA Translation: BAA15882.1 |
PIRi | G64969 |
RefSeqi | NP_416544.1, NC_000913.3 WP_001023610.1, NZ_LN832404.1 |
3D structure databases
SMRi | P37760 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261549, 180 interactors |
IntActi | P37760, 5 interactors |
STRINGi | 511145.b2040 |
Proteomic databases
jPOSTi | P37760 |
PaxDbi | P37760 |
PRIDEi | P37760 |
Genome annotation databases
EnsemblBacteriai | AAC75101; AAC75101; b2040 BAA15882; BAA15882; BAA15882 |
GeneIDi | 947117 |
KEGGi | ecj:JW2025 eco:b2040 |
PATRICi | fig|511145.12.peg.2117 |
Organism-specific databases
EchoBASEi | EB2310 |
Phylogenomic databases
eggNOGi | COG1091, Bacteria |
HOGENOMi | CLU_045518_1_0_6 |
InParanoidi | P37760 |
PhylomeDBi | P37760 |
Enzyme and pathway databases
UniPathwayi | UPA00124 UPA00281 |
BioCyci | EcoCyc:DTDPDEHYRHAMREDUCT-MONOMER |
Miscellaneous databases
PROi | PR:P37760 |
Family and domain databases
InterProi | View protein in InterPro IPR005913, dTDP_dehydrorham_reduct IPR036291, NAD(P)-bd_dom_sf IPR029903, RmlD-like-bd |
PANTHERi | PTHR10491, PTHR10491, 1 hit |
Pfami | View protein in Pfam PF04321, RmlD_sub_bind, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01214, rmlD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RMLD_ECOLI | |
Accessioni | P37760Primary (citable) accession number: P37760 Secondary accession number(s): P76377 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families