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Protein

dTDP-glucose 4,6-dehydratase 1

Gene

rfbB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction.By similarity

Catalytic activityi

dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O.By similarity

Cofactori

NAD+By similarityNote: Binds 1 NAD+ per subunit.By similarity

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84Substrate; via carbonyl oxygenBy similarity1
Binding sitei99NADBy similarity1
Binding sitei133SubstrateBy similarity1
Active sitei134Proton donorBy similarity1
Active sitei135Proton acceptorBy similarity1
Active sitei167Proton acceptorBy similarity1
Binding sitei196SubstrateBy similarity1
Binding sitei197NAD; via amide nitrogenBy similarity1
Binding sitei231SubstrateBy similarity1
Binding sitei266SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2
Nucleotide bindingi32 – 35NADBy similarity4
Nucleotide bindingi58 – 59NADBy similarity2
Nucleotide bindingi80 – 84NADBy similarity5
Nucleotide bindingi167 – 171NADBy similarity5

GO - Molecular functioni

  • coenzyme binding Source: EcoCyc
  • dTDP-glucose 4,6-dehydratase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processLipopolysaccharide biosynthesis
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:DTDPGLUCDEHYDRAT-MONOMER
MetaCyc:DTDPGLUCDEHYDRAT-MONOMER
UniPathwayiUPA00124
UPA00281

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-glucose 4,6-dehydratase 1By similarity (EC:4.2.1.46By similarity)
Gene namesi
Name:rfbB
Synonyms:rmlB
Ordered Locus Names:b2041, JW2026
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12412 rfbB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001832381 – 361dTDP-glucose 4,6-dehydratase 1Add BLAST361

Proteomic databases

EPDiP37759
PaxDbiP37759
PRIDEiP37759

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259679, 184 interactors
DIPiDIP-10680N
IntActiP37759, 7 interactors
STRINGi316385.ECDH10B_2191

Structurei

3D structure databases

ProteinModelPortaliP37759
SMRiP37759
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni206 – 207Substrate bindingBy similarity2
Regioni222 – 224Substrate bindingBy similarity3
Regioni296 – 300Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.By similarity

Phylogenomic databases

eggNOGiENOG4105C1B Bacteria
COG1088 LUCA
HOGENOMiHOG000168006
InParanoidiP37759
KOiK01710
OMAiEWCQHVQ
PhylomeDBiP37759

Family and domain databases

InterProiView protein in InterPro
IPR005888 dTDP_Gluc_deHydtase
IPR016040 NAD(P)-bd_dom
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF16363 GDP_Man_Dehyd, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01181 dTDP_gluc_dehyt, 1 hit

Sequencei

Sequence statusi: Complete.

P37759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILVTGGAG FIGSAVVRHI INNTQDSVVN VDKLTYAGNR ESLADVSDSE
60 70 80 90 100
RYVFEHADIC DAPAMARIFA QHQPDAVMHL AAESHVDRSI TGPAAFIETN
110 120 130 140 150
IVGTYVLLEA ARNYWSALDS DKKNSFRFHH ISTDEVYGDL PHPDEVNNTE
160 170 180 190 200
ELPLFTETTA YAPSSPYSAS KASSDHLVRA WKRTYGLPTI VTNCSNNYGP
210 220 230 240 250
YHFPEKLIPL VILNALEGKA LPIYGKGDQI RDWLYVEDHA RALYTVVTEG
260 270 280 290 300
KAGETYNIGG HNEKKNIDVV LTICDLLDEI VPKEKSYREQ ITYVADRPGH
310 320 330 340 350
DRRYAIDAEK IGRALGWKPQ ETFESGIRKT VEWYLSNTKW VDNVKSGAYQ
360
SWIEQNYEGR Q
Length:361
Mass (Da):40,558
Last modified:November 1, 1997 - v2
Checksum:iC3F64643271C14C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123K → E in AAB88398 (PubMed:7517391).Curated1
Sequence conflicti250G → V in AAB88398 (PubMed:7517391).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09876 Genomic DNA Translation: AAB88398.1
U00096 Genomic DNA Translation: AAC75102.1
AP009048 Genomic DNA Translation: BAA15883.1
PIRiH64969
RefSeqiNP_416545.1, NC_000913.3
WP_000699460.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75102; AAC75102; b2041
BAA15883; BAA15883; BAA15883
GeneIDi945276
KEGGiecj:JW2026
eco:b2041
PATRICifig|1411691.4.peg.210

Similar proteinsi

Entry informationi

Entry nameiRMLB1_ECOLI
AccessioniPrimary (citable) accession number: P37759
Secondary accession number(s): P78082
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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