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Entry version 138 (05 Jun 2019)
Sequence version 3 (01 Nov 1997)
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Protein

Nitrate/nitrite transporter NarU

Gene

narU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes nitrate uptake, nitrite uptake and nitrite export across the cytoplasmic membrane. May function as a nitrate/H+ and nitrite/H+ channel. Could confer a selective advantage during severe nutrient starvation or slow growth.4 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processNitrate assimilation, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:NARU-MONOMER
ECOL316407:JW1464-MONOMER
MetaCyc:NARU-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.1.8.10 the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nitrate/nitrite transporter NarU
Alternative name(s):
Nitrite extrusion protein 2
Nitrite facilitator 2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:narU
Synonyms:yddF
Ordered Locus Names:b1469, JW1464
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG12153 narU

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 35CytoplasmicSequence analysisAdd BLAST35
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 76PeriplasmicSequence analysisAdd BLAST20
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Topological domaini98 – 101CytoplasmicSequence analysis4
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123 – 125PeriplasmicSequence analysis3
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 180CytoplasmicSequence analysisAdd BLAST34
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 206PeriplasmicSequence analysis5
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 258CytoplasmicSequence analysisAdd BLAST31
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21
Topological domaini280 – 287PeriplasmicSequence analysis8
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Topological domaini309 – 317CytoplasmicSequence analysis9
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 344PeriplasmicSequence analysis6
Transmembranei345 – 365HelicalSequence analysisAdd BLAST21
Topological domaini366 – 401CytoplasmicSequence analysisAdd BLAST36
Transmembranei402 – 422HelicalSequence analysisAdd BLAST21
Topological domaini423 – 432PeriplasmicSequence analysis10
Transmembranei433 – 453HelicalSequence analysisAdd BLAST21
Topological domaini454 – 462CytoplasmicSequence analysis9

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi87R → F, H, K, L, N, P or Q: Loss of activity. 2 Publications1
Mutagenesisi99G → A: No change in activity. 1 Publication1
Mutagenesisi99G → T: Decrease in activity. 1 Publication1
Mutagenesisi113P → A: No change in activity. 1 Publication1
Mutagenesisi113P → C or L: Decrease in activity. 1 Publication1
Mutagenesisi139G → E or I: Loss of activity. 1 Publication1
Mutagenesisi145F → E or W: Loss of activity. 1 Publication1
Mutagenesisi162G → A or S: Loss of activity. 1 Publication1
Mutagenesisi172G → A: No change in activity. 1 Publication1
Mutagenesisi172G → V: Loss of activity. 1 Publication1
Mutagenesisi175G → A or S: Loss of activity. 1 Publication1
Mutagenesisi261Y → N: No change in activity. 1 Publication1
Mutagenesisi266G → A, P or T: Loss of activity. 1 Publication1
Mutagenesisi303R → C, D, K, L, N, P or Q: Loss of activity. 2 Publications1
Mutagenesisi307G → L: Loss of activity. 1 Publication1
Mutagenesisi311D → G or K: Loss of activity. 1 Publication1
Mutagenesisi405G → A, L or V: Loss of activity. 1 Publication1
Mutagenesisi414G → L: No change in activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000967291 – 462Nitrate/nitrite transporter NarUAdd BLAST462

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P37758

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P37758

PRoteomics IDEntifications database

More...
PRIDEi
P37758

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expressed preferentially during the stationary phase in the absence of nitrate.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4260180, 13 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b1469

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P37758

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4107QND Bacteria
COG2223 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000241722

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P37758

KEGG Orthology (KO)

More...
KOi
K02575

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P37758

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011701 MFS
IPR036259 MFS_trans_sf
IPR004737 NO3_transporter

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07690 MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473 SSF103473, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00886 2A0108, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P37758-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALQNEKNSR YLLRDWKPEN PAFWENKGKH IARRNLWISV SCLLLAFCVW
60 70 80 90 100
MLFSAVTVNL NKIGFNFTTD QLFLLTALPS VSGALLRVPY SFMVPIFGGR
110 120 130 140 150
RWTVFSTAIL IIPCVWLGIA VQNPNTPFGI FIVIALLCGF AGANFASSMG
160 170 180 190 200
NISFFFPKAK QGSALGINGG LGNLGVSVMQ LVAPLVIFVP VFAFLGVNGV
210 220 230 240 250
PQADGSVMSL ANAAWIWVPL LAIATIAAWS GMNDIASSRA SIADQLPVLQ
260 270 280 290 300
RLHLWLLSLL YLATFGSFIG FSAGFAMLAK TQFPDVNILR LAFFGPFIGA
310 320 330 340 350
IARSVGGAIS DKFGGVRVTL INFIFMAIFS ALLFLTLPGT GSGNFIAFYA
360 370 380 390 400
VFMGLFLTAG LGSGSTFQMI AVIFRQITIY RVKMKGGSDE QAHKEAVTET
410 420 430 440 450
AAALGFISAI GAVGGFFIPQ AFGMSLNMTG SPVGAMKVFL IFYIVCVLLT
460
WLVYGRRKFS QK
Length:462
Mass (Da):49,890
Last modified:November 1, 1997 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i33FBE3C140FBC4DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti290Missing (Ref. 1) Curated1
Sequence conflicti293F → FR (Ref. 1) Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X94992 Genomic DNA Translation: CAA64448.1
U00096 Genomic DNA Translation: AAD13433.1
AP009048 Genomic DNA Translation: BAA15118.1
X17110 Genomic DNA No translation available.

Protein sequence database of the Protein Information Resource

More...
PIRi
H64899 S11431

NCBI Reference Sequences

More...
RefSeqi
NP_415986.1, NC_000913.3
WP_001207901.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAD13433; AAD13433; b1469
BAA15118; BAA15118; BAA15118

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
945799

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW1464
eco:b1469

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.799

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94992 Genomic DNA Translation: CAA64448.1
U00096 Genomic DNA Translation: AAD13433.1
AP009048 Genomic DNA Translation: BAA15118.1
X17110 Genomic DNA No translation available.
PIRiH64899 S11431
RefSeqiNP_415986.1, NC_000913.3
WP_001207901.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IU8X-ray3.11A/B1-462[»]
4IU9X-ray3.00A/B1-462[»]
SMRiP37758
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4260180, 13 interactors
STRINGi511145.b1469

Protein family/group databases

TCDBi2.A.1.8.10 the major facilitator superfamily (mfs)

Proteomic databases

jPOSTiP37758
PaxDbiP37758
PRIDEiP37758

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD13433; AAD13433; b1469
BAA15118; BAA15118; BAA15118
GeneIDi945799
KEGGiecj:JW1464
eco:b1469
PATRICifig|1411691.4.peg.799

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB2073
EcoGeneiEG12153 narU

Phylogenomic databases

eggNOGiENOG4107QND Bacteria
COG2223 LUCA
HOGENOMiHOG000241722
InParanoidiP37758
KOiK02575
PhylomeDBiP37758

Enzyme and pathway databases

BioCyciEcoCyc:NARU-MONOMER
ECOL316407:JW1464-MONOMER
MetaCyc:NARU-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P37758

Family and domain databases

InterProiView protein in InterPro
IPR011701 MFS
IPR036259 MFS_trans_sf
IPR004737 NO3_transporter
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00886 2A0108, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNARU_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P37758
Secondary accession number(s): P77696
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: June 5, 2019
This is version 138 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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