UniProtKB - P37744 (RMLA1_ECOLI)
Protein
Glucose-1-phosphate thymidylyltransferase 1
Gene
rfbA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.1 Publication
Miscellaneous
Both catalyzed reactions, i.e. dTDP-glucose biosynthesis and pyrophosphorolysis, follow a sequential ordered bi-bi catalytic mechanism.1 Publication
Caution
Most extant K-12 lines do not express O-antigen because of mutations in dTDP-rhamnose biosynthetic genes or in the rhamnosyltransferase gene wbbL.Curated
Catalytic activityi
- EC:2.7.7.241 Publication
Cofactori
Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication
Kineticsi
- KM=20.5 µM for dTTP1 Publication
- KM=34 µM for glucose 1-phosphate1 Publication
- KM=95 µM for dTDP-glucose1 Publication
- KM=154 µM for PPi1 Publication
- Vmax=194 µmol/min/mg enzyme for the forward reaction1 Publication
- Vmax=360 µmol/min/mg enzyme for the reverse reaction1 Publication
pH dependencei
Optimum pH is 8.0-8.5. Active from pH 6.0 to 10.0.1 Publication
: dTDP-L-rhamnose biosynthesis Pathwayi
This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.
Pathwayi: LPS O-antigen biosynthesis
This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 111 | MagnesiumBy similarity | 1 | |
Metal bindingi | 226 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- glucose-1-phosphate thymidylyltransferase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- metal ion binding Source: EcoCyc
GO - Biological processi
- dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
- extracellular polysaccharide biosynthetic process Source: InterPro
- O antigen biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Lipopolysaccharide biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:DTDPGLUCOSEPP-MONOMER MetaCyc:DTDPGLUCOSEPP-MONOMER |
UniPathwayi | UPA00124 UPA00281 |
Names & Taxonomyi
Protein namesi | Recommended name: Glucose-1-phosphate thymidylyltransferase 1 (EC:2.7.7.241 Publication)Short name: G1P-TT 1 Alternative name(s): dTDP-glucose pyrophosphorylase 1 dTDP-glucose synthase 1 |
Gene namesi | Name:rfbA Synonyms:rmlA, rmlA1 Ordered Locus Names:b2039, JW2024 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207991 | 1 – 293 | Glucose-1-phosphate thymidylyltransferase 1Add BLAST | 293 |
Proteomic databases
jPOSTi | P37744 |
PaxDbi | P37744 |
PRIDEi | P37744 |
2D gel databases
SWISS-2DPAGEi | P37744 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259680, 190 interactors |
IntActi | P37744, 9 interactors |
STRINGi | 511145.b2039 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37744 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37744 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glucose-1-phosphate thymidylyltransferase family.Curated
Phylogenomic databases
eggNOGi | COG1209, Bacteria |
HOGENOMi | CLU_029499_9_0_6 |
InParanoidi | P37744 |
PhylomeDBi | P37744 |
Family and domain databases
CDDi | cd02538, G1P_TT_short, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005907, G1P_thy_trans_s IPR005835, NTP_transferase_dom IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR43532, PTHR43532, 1 hit |
Pfami | View protein in Pfam PF00483, NTP_transferase, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR01207, rmlA, 1 hit |
i Sequence
Sequence statusi: Complete.
P37744-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKMRKGIILA GGSGTRLYPV TMAVSKQLLP IYDKPMIYYP LSTLMLAGIR
60 70 80 90 100
DILIISTPQD TPRFQQLLGD GSQWGLNLQY KVQPSPDGLA QAFIIGEEFI
110 120 130 140 150
GGDDCALVLG DNIFYGHDLP KLMEAAVNKE SGATVFAYHV NDPERYGVVE
160 170 180 190 200
FDKNGTAISL EEKPLEPKSN YAVTGLYFYD NDVVQMAKNL KPSARGELEI
210 220 230 240 250
TDINRIYLEQ GRLSVAMMGR GYAWLDTGTH QSLIEASNFI ATIEERQGLK
260 270 280 290
VSCPEEIAFR KGFIDVEQVR KLAVPLIKNN YGQYLYKMTK DSN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 247 | Q → P in AAC31629 (PubMed:7517390).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09876 Genomic DNA Translation: AAB88400.1 U00096 Genomic DNA Translation: AAC75100.1 AP009048 Genomic DNA Translation: BAA15881.1 U03041 Genomic DNA Translation: AAC31629.1 |
PIRi | F64969 |
RefSeqi | NP_416543.1, NC_000913.3 WP_000783975.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75100; AAC75100; b2039 BAA15881; BAA15881; BAA15881 |
GeneIDi | 945154 |
KEGGi | ecj:JW2024 eco:b2039 |
PATRICi | fig|1411691.4.peg.212 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09876 Genomic DNA Translation: AAB88400.1 U00096 Genomic DNA Translation: AAC75100.1 AP009048 Genomic DNA Translation: BAA15881.1 U03041 Genomic DNA Translation: AAC31629.1 |
PIRi | F64969 |
RefSeqi | NP_416543.1, NC_000913.3 WP_000783975.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H5R | X-ray | 1.90 | A/B/C/D | 1-293 | [»] | |
1H5S | X-ray | 2.30 | A/B/C/D | 1-293 | [»] | |
1H5T | X-ray | 1.90 | A/B/C/D | 1-293 | [»] | |
SMRi | P37744 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259680, 190 interactors |
IntActi | P37744, 9 interactors |
STRINGi | 511145.b2039 |
2D gel databases
SWISS-2DPAGEi | P37744 |
Proteomic databases
jPOSTi | P37744 |
PaxDbi | P37744 |
PRIDEi | P37744 |
Genome annotation databases
EnsemblBacteriai | AAC75100; AAC75100; b2039 BAA15881; BAA15881; BAA15881 |
GeneIDi | 945154 |
KEGGi | ecj:JW2024 eco:b2039 |
PATRICi | fig|1411691.4.peg.212 |
Organism-specific databases
EchoBASEi | EB1921 |
Phylogenomic databases
eggNOGi | COG1209, Bacteria |
HOGENOMi | CLU_029499_9_0_6 |
InParanoidi | P37744 |
PhylomeDBi | P37744 |
Enzyme and pathway databases
UniPathwayi | UPA00124 UPA00281 |
BioCyci | EcoCyc:DTDPGLUCOSEPP-MONOMER MetaCyc:DTDPGLUCOSEPP-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P37744 |
PROi | PR:P37744 |
Family and domain databases
CDDi | cd02538, G1P_TT_short, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005907, G1P_thy_trans_s IPR005835, NTP_transferase_dom IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR43532, PTHR43532, 1 hit |
Pfami | View protein in Pfam PF00483, NTP_transferase, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR01207, rmlA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RMLA1_ECOLI | |
Accessioni | P37744Primary (citable) accession number: P37744 Secondary accession number(s): P78081 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families