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Protein

Glucose-1-phosphate thymidylyltransferase 1

Gene

rfbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.1 Publication

Miscellaneous

Both catalyzed reactions, i.e. dTDP-glucose biosynthesis and pyrophosphorolysis, follow a sequential ordered bi-bi catalytic mechanism.1 Publication

Caution

Most extant K-12 lines do not express O-antigen because of mutations in dTDP-rhamnose biosynthetic genes or in the rhamnosyltransferase gene wbbL.Curated

Catalytic activityi

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

  1. KM=20.5 µM for dTTP1 Publication
  2. KM=34 µM for glucose 1-phosphate1 Publication
  3. KM=95 µM for dTDP-glucose1 Publication
  4. KM=154 µM for PPi1 Publication
  1. Vmax=194 µmol/min/mg enzyme for the forward reaction1 Publication
  2. Vmax=360 µmol/min/mg enzyme for the reverse reaction1 Publication

pH dependencei

Optimum pH is 8.0-8.5. Active from pH 6.0 to 10.0.1 Publication

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111MagnesiumBy similarity1
Metal bindingi226MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processLipopolysaccharide biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:DTDPGLUCOSEPP-MONOMER
MetaCyc:DTDPGLUCOSEPP-MONOMER
UniPathwayi
UPA00124

UPA00281

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate thymidylyltransferase 1 (EC:2.7.7.241 Publication)
Short name:
G1P-TT 1
Alternative name(s):
dTDP-glucose pyrophosphorylase 1
dTDP-glucose synthase 1
Gene namesi
Name:rfbA
Synonyms:rmlA, rmlA1
Ordered Locus Names:b2039, JW2024
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11978 rfbA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002079911 – 293Glucose-1-phosphate thymidylyltransferase 1Add BLAST293

Proteomic databases

EPDiP37744
PaxDbiP37744
PRIDEiP37744

2D gel databases

SWISS-2DPAGEiP37744

Interactioni

Subunit structurei

Homotetramer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4259680, 190 interactors
IntActiP37744, 9 interactors
STRINGi316385.ECDH10B_2189

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP37744
SMRiP37744
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP37744

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108I19 Bacteria
COG1209 LUCA
HOGENOMiHOG000283473
InParanoidiP37744
KOiK00973
OMAiGPYPMIY
PhylomeDBiP37744

Family and domain databases

CDDicd02538 G1P_TT_short, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR005907 G1P_thy_trans_s
IPR005835 NTP_transferase_dom
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR43532 PTHR43532, 1 hit
PfamiView protein in Pfam
PF00483 NTP_transferase, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01207 rmlA, 1 hit

Sequencei

Sequence statusi: Complete.

P37744-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKMRKGIILA GGSGTRLYPV TMAVSKQLLP IYDKPMIYYP LSTLMLAGIR
60 70 80 90 100
DILIISTPQD TPRFQQLLGD GSQWGLNLQY KVQPSPDGLA QAFIIGEEFI
110 120 130 140 150
GGDDCALVLG DNIFYGHDLP KLMEAAVNKE SGATVFAYHV NDPERYGVVE
160 170 180 190 200
FDKNGTAISL EEKPLEPKSN YAVTGLYFYD NDVVQMAKNL KPSARGELEI
210 220 230 240 250
TDINRIYLEQ GRLSVAMMGR GYAWLDTGTH QSLIEASNFI ATIEERQGLK
260 270 280 290
VSCPEEIAFR KGFIDVEQVR KLAVPLIKNN YGQYLYKMTK DSN
Length:293
Mass (Da):32,694
Last modified:November 1, 1997 - v2
Checksum:iBA895362D1C5CA55
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti247Q → P in AAC31629 (PubMed:7517390).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09876 Genomic DNA Translation: AAB88400.1
U00096 Genomic DNA Translation: AAC75100.1
AP009048 Genomic DNA Translation: BAA15881.1
U03041 Genomic DNA Translation: AAC31629.1
PIRiF64969
RefSeqiNP_416543.1, NC_000913.3
WP_000783975.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75100; AAC75100; b2039
BAA15881; BAA15881; BAA15881
GeneIDi945154
KEGGiecj:JW2024
eco:b2039
PATRICifig|1411691.4.peg.212

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09876 Genomic DNA Translation: AAB88400.1
U00096 Genomic DNA Translation: AAC75100.1
AP009048 Genomic DNA Translation: BAA15881.1
U03041 Genomic DNA Translation: AAC31629.1
PIRiF64969
RefSeqiNP_416543.1, NC_000913.3
WP_000783975.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H5RX-ray1.90A/B/C/D1-293[»]
1H5SX-ray2.30A/B/C/D1-293[»]
1H5TX-ray1.90A/B/C/D1-293[»]
ProteinModelPortaliP37744
SMRiP37744
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259680, 190 interactors
IntActiP37744, 9 interactors
STRINGi316385.ECDH10B_2189

2D gel databases

SWISS-2DPAGEiP37744

Proteomic databases

EPDiP37744
PaxDbiP37744
PRIDEiP37744

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75100; AAC75100; b2039
BAA15881; BAA15881; BAA15881
GeneIDi945154
KEGGiecj:JW2024
eco:b2039
PATRICifig|1411691.4.peg.212

Organism-specific databases

EchoBASEiEB1921
EcoGeneiEG11978 rfbA

Phylogenomic databases

eggNOGiENOG4108I19 Bacteria
COG1209 LUCA
HOGENOMiHOG000283473
InParanoidiP37744
KOiK00973
OMAiGPYPMIY
PhylomeDBiP37744

Enzyme and pathway databases

UniPathwayi
UPA00124

UPA00281

BioCyciEcoCyc:DTDPGLUCOSEPP-MONOMER
MetaCyc:DTDPGLUCOSEPP-MONOMER

Miscellaneous databases

EvolutionaryTraceiP37744
PROiPR:P37744

Family and domain databases

CDDicd02538 G1P_TT_short, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR005907 G1P_thy_trans_s
IPR005835 NTP_transferase_dom
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR43532 PTHR43532, 1 hit
PfamiView protein in Pfam
PF00483 NTP_transferase, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR01207 rmlA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRMLA1_ECOLI
AccessioniPrimary (citable) accession number: P37744
Secondary accession number(s): P78081
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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