UniProtKB - P37700 (GUNG_RUMCH)
Protein
Endoglucanase G
Gene
celCCG
Organism
Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) (Clostridium cellulolyticum)
Status
Functioni
The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4
: cellulose degradation Pathwayi
This protein is involved in the pathway cellulose degradation, which is part of Glycan metabolism.View all proteins of this organism that are known to be involved in the pathway cellulose degradation and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 93 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 408 | PROSITE-ProRule annotation | 1 | |
Active sitei | 446 | PROSITE-ProRule annotation | 1 | |
Active sitei | 455 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cellulase activity Source: UniProtKB-EC
- cellulose binding Source: InterPro
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Enzyme and pathway databases
BioCyci | CCEL394503:G1GUL-747-MONOMER |
UniPathwayi | UPA00696 |
Protein family/group databases
CAZyi | CBM3, Carbohydrate-Binding Module Family 3 GH9, Glycoside Hydrolase Family 9 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase G (EC:3.2.1.4)Alternative name(s): Cellulase G EGCCG Endo-1,4-beta-glucanase G |
Gene namesi | Name:celCCG Ordered Locus Names:Ccel_0731 |
Organismi | Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) (Clostridium cellulolyticum) |
Taxonomic identifieri | 394503 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Clostridiales › Hungateiclostridiaceae › Ruminiclostridium › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
DrugBanki | DB03859, 1-thio-beta-D-glucopyranose DB03584, 4-Thio-beta-D-glucopyranose DB02379, Beta-D-Glucose DB02061, Cellobiose DB02361, S-Methyl Thiocysteine Group |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | Sequence analysisAdd BLAST | 35 | |
ChainiPRO_0000007947 | 36 – 725 | Endoglucanase GAdd BLAST | 690 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37700 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37700 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 489 – 650 | CBM3PROSITE-ProRule annotationAdd BLAST | 162 | |
Domaini | 658 – 724 | DockerinPROSITE-ProRule annotationAdd BLAST | 67 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG4733, Bacteria |
HOGENOMi | CLU_008926_0_2_9 |
OMAi | RWLDYWT |
OrthoDBi | 1226595at2 |
Family and domain databases
Gene3Di | 1.10.1330.10, 1 hit 1.50.10.10, 1 hit 2.60.40.710, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR001956, CBM3 IPR036966, CBM3_sf IPR002105, Dockerin_1_rpt IPR016134, Dockerin_dom IPR036439, Dockerin_dom_sf IPR001701, Glyco_hydro_9 IPR033126, Glyco_hydro_9_Asp/Glu_AS IPR018221, Glyco_hydro_9_His_AS |
Pfami | View protein in Pfam PF00942, CBM_3, 1 hit PF00404, Dockerin_1, 1 hit PF00759, Glyco_hydro_9, 1 hit |
SMARTi | View protein in SMART SM01067, CBM_3, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF49384, SSF49384, 1 hit SSF63446, SSF63446, 1 hit |
PROSITEi | View protein in PROSITE PS51172, CBM3, 1 hit PS00448, CLOS_CELLULOSOME_RPT, 1 hit PS51766, DOCKERIN, 1 hit PS00018, EF_HAND_1, 1 hit PS60032, GH9_1, 1 hit PS00592, GH9_2, 1 hit PS00698, GH9_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P37700-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKTKRKLTK AIGVALSISI LSSLVSFIPQ TNTYAAGTYN YGEALQKSIM
60 70 80 90 100
FYEFQRSGDL PADKRDNWRD DSGMKDGSDV GVDLTGGWYD AGDHVKFNLP
110 120 130 140 150
MSYTSAMLAW SLYEDKDAYD KSGQTKYIMD GIKWANDYFI KCNPTPGVYY
160 170 180 190 200
YQVGDGGKDH SWWGPAEVMQ MERPSFKVDA SKPGSAVCAS TAASLASAAV
210 220 230 240 250
VFKSSDPTYA EKCISHAKNL FDMADKAKSD AGYTAASGYY SSSSFYDDLS
260 270 280 290 300
WAAVWLYLAT NDSTYLDKAE SYVPNWGKEQ QTDIIAYKWG QCWDDVHYGA
310 320 330 340 350
ELLLAKLTNK QLYKDSIEMN LDFWTTGVNG TRVSYTPKGL AWLFQWGSLR
360 370 380 390 400
HATTQAFLAG VYAEWEGCTP SKVSVYKDFL KSQIDYALGS TGRSFVVGYG
410 420 430 440 450
VNPPQHPHHR TAHGSWTDQM TSPTYHRHTI YGALVGGPDN ADGYTDEINN
460 470 480 490 500
YVNNEIACDY NAGFTGALAK MYKHSGGDPI PNFKAIEKIT NDEVIIKAGL
510 520 530 540 550
NSTGPNYTEI KAVVYNQTGW PARVTDKISF KYFMDLSEIV AAGIDPLSLV
560 570 580 590 600
TSSNYSEGKN TKVSGVLPWD VSNNVYYVNV DLTGENIYPG GQSACRREVQ
610 620 630 640 650
FRIAAPQGTT YWNPKNDFSY DGLPTTSTVN TVTNIPVYDN GVKVFGNEPA
660 670 680 690 700
GGSENPDPEI LYGDVNSDKN VDALDFAALK KYLLGGTSSI DVKAADTYKD
710 720
GNIDAIDMAT LKKYLLGTIT QLPQG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 609 – 610 | TT → RR in AAA73868 (PubMed:1398087).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87018 Genomic DNA Translation: AAA73868.1 CP001348 Genomic DNA Translation: ACL75110.1 |
PIRi | JC1300 |
RefSeqi | WP_015924277.1, NC_011898.1 |
Genome annotation databases
EnsemblBacteriai | ACL75110; ACL75110; Ccel_0731 |
KEGGi | cce:Ccel_0731 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87018 Genomic DNA Translation: AAA73868.1 CP001348 Genomic DNA Translation: ACL75110.1 |
PIRi | JC1300 |
RefSeqi | WP_015924277.1, NC_011898.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G87 | X-ray | 1.60 | A/B | 36-649 | [»] | |
1GA2 | X-ray | 1.70 | A/B | 36-649 | [»] | |
1K72 | X-ray | 1.80 | A/B | 36-649 | [»] | |
1KFG | X-ray | 1.90 | A/B | 36-649 | [»] | |
SMRi | P37700 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 394503.Ccel_0731 |
Chemistry databases
DrugBanki | DB03859, 1-thio-beta-D-glucopyranose DB03584, 4-Thio-beta-D-glucopyranose DB02379, Beta-D-Glucose DB02061, Cellobiose DB02361, S-Methyl Thiocysteine Group |
Protein family/group databases
CAZyi | CBM3, Carbohydrate-Binding Module Family 3 GH9, Glycoside Hydrolase Family 9 |
Genome annotation databases
EnsemblBacteriai | ACL75110; ACL75110; Ccel_0731 |
KEGGi | cce:Ccel_0731 |
Phylogenomic databases
eggNOGi | COG4733, Bacteria |
HOGENOMi | CLU_008926_0_2_9 |
OMAi | RWLDYWT |
OrthoDBi | 1226595at2 |
Enzyme and pathway databases
UniPathwayi | UPA00696 |
BioCyci | CCEL394503:G1GUL-747-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P37700 |
Family and domain databases
Gene3Di | 1.10.1330.10, 1 hit 1.50.10.10, 1 hit 2.60.40.710, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR001956, CBM3 IPR036966, CBM3_sf IPR002105, Dockerin_1_rpt IPR016134, Dockerin_dom IPR036439, Dockerin_dom_sf IPR001701, Glyco_hydro_9 IPR033126, Glyco_hydro_9_Asp/Glu_AS IPR018221, Glyco_hydro_9_His_AS |
Pfami | View protein in Pfam PF00942, CBM_3, 1 hit PF00404, Dockerin_1, 1 hit PF00759, Glyco_hydro_9, 1 hit |
SMARTi | View protein in SMART SM01067, CBM_3, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF49384, SSF49384, 1 hit SSF63446, SSF63446, 1 hit |
PROSITEi | View protein in PROSITE PS51172, CBM3, 1 hit PS00448, CLOS_CELLULOSOME_RPT, 1 hit PS51766, DOCKERIN, 1 hit PS00018, EF_HAND_1, 1 hit PS60032, GH9_1, 1 hit PS00592, GH9_2, 1 hit PS00698, GH9_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GUNG_RUMCH | |
Accessioni | P37700Primary (citable) accession number: P37700 Secondary accession number(s): B8I7V3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | May 26, 2009 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries