UniProtKB - P37699 (GUNC_RUMCH)
Protein
Endoglucanase C
Gene
celCCC
Organism
Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) (Clostridium cellulolyticum)
Status
Functioni
The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4
: cellulose degradation Pathwayi
This protein is involved in the pathway cellulose degradation, which is part of Glycan metabolism.View all proteins of this organism that are known to be involved in the pathway cellulose degradation and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 99 | Proton donorBy similarity | 1 | |
Active sitei | 155 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cellulase activity Source: UniProtKB-EC
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Enzyme and pathway databases
BioCyci | CCEL394503:G1GUL-746-MONOMER |
UniPathwayi | UPA00696 |
Protein family/group databases
CAZyi | GH8, Glycoside Hydrolase Family 8 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase C (EC:3.2.1.4)Alternative name(s): Cellulase C EGCCC Endo-1,4-beta-glucanase C |
Gene namesi | Name:celCCC Ordered Locus Names:Ccel_0730 |
Organismi | Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) (Clostridium cellulolyticum) |
Taxonomic identifieri | 394503 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Clostridiales › Hungateiclostridiaceae › Ruminiclostridium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | 1 PublicationAdd BLAST | 32 | |
ChainiPRO_0000007936 | 33 – 460 | Endoglucanase CAdd BLAST | 428 |
Post-translational modificationi
There are two forms of the cellulase. The shorter form lacks probably the C-terminal reiterated domains.
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
STRINGi | 394503.Ccel_0730 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 400 – 460 | DockerinPROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 8 (cellulase D) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3405, Bacteria |
HOGENOMi | CLU_036185_0_0_9 |
OMAi | EAHGYGM |
OrthoDBi | 636673at2 |
Family and domain databases
Gene3Di | 1.10.1330.10, 1 hit 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR002105, Dockerin_1_rpt IPR016134, Dockerin_dom IPR036439, Dockerin_dom_sf IPR018247, EF_Hand_1_Ca_BS IPR002037, Glyco_hydro_8 IPR019834, Glyco_hydro_8_CS |
Pfami | View protein in Pfam PF00404, Dockerin_1, 1 hit PF01270, Glyco_hydro_8, 1 hit |
PRINTSi | PR00735, GLHYDRLASE8 |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF63446, SSF63446, 1 hit |
PROSITEi | View protein in PROSITE PS00448, CLOS_CELLULOSOME_RPT, 1 hit PS51766, DOCKERIN, 1 hit PS00018, EF_HAND_1, 2 hits PS00812, GLYCOSYL_HYDROL_F8, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P37699-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKGSSLKRF KSLVMAAIFS VSIISTAIAS SAADQIPFPY DAKYPNGAYS
60 70 80 90 100
CLADSQSIGN NLVRSEWEQW KSAHITSNGA RGYKRVQRDA STNYDTVSEG
110 120 130 140 150
LGYGLLLSVY FGEQQLFDDL YRYVKVFLNS NGLMSWRIDS SGNIMGKDSI
160 170 180 190 200
GAATDADEDI AVSLVFAHKK WGTSGGFNYQ TEAKNYINNI YNKMVEPGTY
210 220 230 240 250
VIKAGDTWGG SNVTNPSYFA PAWYRIFADF TGNSGWINVA NKCYEIADKA
260 270 280 290 300
RNSNTGLVPD WCTANGTPAS GQGFDFYYDA IRYQWRAAID YSWYGTAKAK
310 320 330 340 350
THCDAISNFF KNIGYANIKD GYTISGSQIS SNHTATFVSC AAAAAMTGTD
360 370 380 390 400
TTYAKNIYNE CVKVKDSGNY TYFGNTLRMM VLLYTTGNFP NLYTYNSQPK
410 420 430 440 450
PDLKGDVNND GAIDALDIAA LKKAILTQTT SNISLTNADM NNDGNIDAID
460
FAQLKVKLLN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 91 | S → T in AAA73867 (PubMed:1398087).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87018 Genomic DNA Translation: AAA73867.1 CP001348 Genomic DNA Translation: ACL75109.1 |
PIRi | JC1299 |
RefSeqi | WP_015924276.1, NC_011898.1 |
Genome annotation databases
EnsemblBacteriai | ACL75109; ACL75109; Ccel_0730 |
KEGGi | cce:Ccel_0730 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M87018 Genomic DNA Translation: AAA73867.1 CP001348 Genomic DNA Translation: ACL75109.1 |
PIRi | JC1299 |
RefSeqi | WP_015924276.1, NC_011898.1 |
3D structure databases
SMRi | P37699 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 394503.Ccel_0730 |
Protein family/group databases
CAZyi | GH8, Glycoside Hydrolase Family 8 |
Genome annotation databases
EnsemblBacteriai | ACL75109; ACL75109; Ccel_0730 |
KEGGi | cce:Ccel_0730 |
Phylogenomic databases
eggNOGi | COG3405, Bacteria |
HOGENOMi | CLU_036185_0_0_9 |
OMAi | EAHGYGM |
OrthoDBi | 636673at2 |
Enzyme and pathway databases
UniPathwayi | UPA00696 |
BioCyci | CCEL394503:G1GUL-746-MONOMER |
Family and domain databases
Gene3Di | 1.10.1330.10, 1 hit 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR002105, Dockerin_1_rpt IPR016134, Dockerin_dom IPR036439, Dockerin_dom_sf IPR018247, EF_Hand_1_Ca_BS IPR002037, Glyco_hydro_8 IPR019834, Glyco_hydro_8_CS |
Pfami | View protein in Pfam PF00404, Dockerin_1, 1 hit PF01270, Glyco_hydro_8, 1 hit |
PRINTSi | PR00735, GLHYDRLASE8 |
SUPFAMi | SSF48208, SSF48208, 1 hit SSF63446, SSF63446, 1 hit |
PROSITEi | View protein in PROSITE PS00448, CLOS_CELLULOSOME_RPT, 1 hit PS51766, DOCKERIN, 1 hit PS00018, EF_HAND_1, 2 hits PS00812, GLYCOSYL_HYDROL_F8, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GUNC_RUMCH | |
Accessioni | P37699Primary (citable) accession number: P37699 Secondary accession number(s): B8I7V2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | May 26, 2009 | |
Last modified: | October 7, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries