UniProtKB - P37680 (SGBE_ECOLI)
Protein
L-ribulose-5-phosphate 4-epimerase SgbE
Gene
sgbE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the interconversion of L-ribulose 5-phosphate (LRu5P) and D-xylulose 5-phosphate (D-Xu5P) via a retroaldol/aldol mechanism (carbon-carbon bond cleavage analogous to a class II aldolase reaction). May be involved in the utilization of 2,3-diketo-L-gulonate.2 Publications
Catalytic activityi
- EC:5.1.3.41 Publication
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Kineticsi
Kcat is 12 sec(-1) with L-ribulose 5-phosphate (LRu5P) as substrate.1 Publication
- KM=705 µM for L-ribulose 5-phosphate (LRu5P)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 76 | ZincBy similarity | 1 | |
Metal bindingi | 95 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 97 | Zinc; via tele nitrogenBy similarity | 1 | |
Active sitei | 120 | Proton donor/acceptorBy similarity | 1 | |
Metal bindingi | 171 | Zinc; via tele nitrogenBy similarity | 1 | |
Active sitei | 229 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- aldehyde-lyase activity Source: GO_Central
- L-ribulose-phosphate 4-epimerase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- L-arabinose catabolic process Source: InterPro
- L-lyxose metabolic process Source: EcoCyc
- pentose catabolic process Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | Carbohydrate metabolism |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12287-MONOMER MetaCyc:EG12287-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: L-ribulose-5-phosphate 4-epimerase SgbE1 Publication (EC:5.1.3.41 Publication)Alternative name(s): Phosphoribulose isomeraseCurated |
Gene namesi | Name:sgbE1 Publication Synonyms:yiaS Ordered Locus Names:b3583, JW3555 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162923 | 1 – 231 | L-ribulose-5-phosphate 4-epimerase SgbEAdd BLAST | 231 |
Proteomic databases
PaxDbi | P37680 |
PRIDEi | P37680 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262553, 10 interactors 852406, 9 interactors |
IntActi | P37680, 13 interactors |
STRINGi | 511145.b3583 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 27 – 28 | Substrate bindingBy similarity | 2 | |
Regioni | 44 – 45 | Substrate bindingBy similarity | 2 | |
Regioni | 74 – 75 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0235, Bacteria |
HOGENOMi | CLU_006033_5_0_6 |
InParanoidi | P37680 |
PhylomeDBi | P37680 |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf IPR004661, AraD |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
TIGRFAMsi | TIGR00760, araD, 1 hit |
i Sequence
Sequence statusi: Complete.
P37680-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEQLKADVL AANLALPAHH LVTFTWGNVS AVDETRQWMV IKPSGVEYDV
60 70 80 90 100
MTADDMVVVE IASGKVVEGS KKPSSDTPTH LALYRRYAEI GGIVHTHSRH
110 120 130 140 150
ATIWSQAGLD LPAWGTTHAD YFYGAIPCTR QMTAEEINGE YEYQTGEVII
160 170 180 190 200
ETFEERGRSP AQIPAVLVHS HGPFAWGKNA ADAVHNAVVL EECAYMGLFS
210 220 230
RQLAPQLPAM QNELLDKHYL RKHGANAYYG Q
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18560.1 U00096 Genomic DNA Translation: AAC76607.1 AP009048 Genomic DNA Translation: BAE77710.1 |
PIRi | S47804 |
RefSeqi | NP_418040.1, NC_000913.3 WP_000893142.1, NZ_SSZK01000041.1 |
Genome annotation databases
EnsemblBacteriai | AAC76607; AAC76607; b3583 BAE77710; BAE77710; BAE77710 |
GeneIDi | 58461587 948099 |
KEGGi | ecj:JW3555 eco:b3583 |
PATRICi | fig|511145.12.peg.3698 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18560.1 U00096 Genomic DNA Translation: AAC76607.1 AP009048 Genomic DNA Translation: BAE77710.1 |
PIRi | S47804 |
RefSeqi | NP_418040.1, NC_000913.3 WP_000893142.1, NZ_SSZK01000041.1 |
3D structure databases
SMRi | P37680 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262553, 10 interactors 852406, 9 interactors |
IntActi | P37680, 13 interactors |
STRINGi | 511145.b3583 |
Proteomic databases
PaxDbi | P37680 |
PRIDEi | P37680 |
Genome annotation databases
EnsemblBacteriai | AAC76607; AAC76607; b3583 BAE77710; BAE77710; BAE77710 |
GeneIDi | 58461587 948099 |
KEGGi | ecj:JW3555 eco:b3583 |
PATRICi | fig|511145.12.peg.3698 |
Organism-specific databases
EchoBASEi | EB2195 |
Phylogenomic databases
eggNOGi | COG0235, Bacteria |
HOGENOMi | CLU_006033_5_0_6 |
InParanoidi | P37680 |
PhylomeDBi | P37680 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12287-MONOMER MetaCyc:EG12287-MONOMER |
Miscellaneous databases
PROi | PR:P37680 |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR001303, Aldolase_II/adducin_N IPR036409, Aldolase_II/adducin_N_sf IPR004661, AraD |
Pfami | View protein in Pfam PF00596, Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007, Aldolase_II, 1 hit |
SUPFAMi | SSF53639, SSF53639, 1 hit |
TIGRFAMsi | TIGR00760, araD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SGBE_ECOLI | |
Accessioni | P37680Primary (citable) accession number: P37680 Secondary accession number(s): Q2M7P6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families