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Protein

Glyoxylate/hydroxypyruvate reductase B

Gene

ghrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively. Can also reduce 2,5-diketo-D-gluconate (25DKG) to 5-keto-D-gluconate (5KDG), 2-keto-D-gluconate (2KDG) to D-gluconate, and 2-keto-L-gulonate (2KLG) to L-idonate (IA), but it is not its physiological function. Inactive towards 2-oxoglutarate, oxaloacetate, pyruvate, 5-keto-D-gluconate, D-fructose and L-sorbose. Activity with NAD is very low.

Catalytic activityi

Glycolate + NADP+ = glyoxylate + NADPH.1 Publication
D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H.1 Publication
D-gluconate + NADP+ = 2-dehydro-D-gluconate + NADPH.1 Publication

Kineticsi

The catalytic efficiency is better for hydroxypyruvate than glyoxylate with NADPH as electron donor.
  1. KM=0.7 mM for hydroxypyruvate (at pH 7.5)2 Publications
  2. KM=1.5 mM for 2-oxo-D-gluconate (at pH 7.5)2 Publications
  3. KM=6.6 mM for glyoxylate (at pH 7.5)2 Publications
  1. Vmax=345 µmol/min/mg enzyme with glyoxylate as substrate (at pH 7)2 Publications
  2. Vmax=123 µmol/min/mg enzyme with hydroxypyruvate as substrate (at pH 7)2 Publications
  3. Vmax=69 µmol/min/mg enzyme with 2-oxo-D-gluconate as substrate (at pH 7)2 Publications

pH dependencei

Optimum pH is 7.5.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei237By similarity1
Active sitei266By similarity1
Active sitei285Proton donorBy similarity1

GO - Molecular functioni

  • gluconate 2-dehydrogenase activity Source: EcoCyc
  • glyoxylate reductase (NADP) activity Source: EcoCyc
  • hydroxypyruvate reductase activity Source: GO_Central
  • NAD binding Source: InterPro

GO - Biological processi

  • D-gluconate metabolic process Source: UniProtKB-KW
  • ketogluconate catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processGluconate utilization
LigandNAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER-43
MetaCyc:MONOMER-43
SABIO-RKiP37666

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxylate/hydroxypyruvate reductase B (EC:1.1.1.79, EC:1.1.1.81)
Alternative name(s):
2-ketoaldonate reductase
2-ketogluconate reductase (EC:1.1.1.215)
Short name:
2KR
Gene namesi
Name:ghrB
Synonyms:tkrA, yiaE
Ordered Locus Names:b3553, JW5656
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12272 ghrB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760291 – 324Glyoxylate/hydroxypyruvate reductase BAdd BLAST324

Proteomic databases

EPDiP37666
PaxDbiP37666
PRIDEiP37666

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
pgmP369382EBI-562547,EBI-542427

Protein-protein interaction databases

BioGridi4259301, 35 interactors
DIPiDIP-10997N
IntActiP37666, 1 interactor
STRINGi316385.ECDH10B_3732

Structurei

3D structure databases

ProteinModelPortaliP37666
SMRiP37666
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C5I Bacteria
COG1052 LUCA
HOGENOMiHOG000136700
InParanoidiP37666
KOiK00090
OMAiKWIAHNG
PhylomeDBiP37666

Family and domain databases

HAMAPiMF_01667 2_Hacid_dh_C_GhrB, 1 hit
InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029753 D-isomer_DH_CS
IPR006140 D-isomer_DH_NAD-bd
IPR023756 Glyo/OHPyrv_Rdtase_B
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00670 D_2_HYDROXYACID_DH_2, 1 hit
PS00671 D_2_HYDROXYACID_DH_3, 1 hit

Sequencei

Sequence statusi: Complete.

P37666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSVILYKA LPDDLLQRLQ EHFTVHQVAN LSPQTVEQNA AIFAEAEGLL
60 70 80 90 100
GSNENVNAAL LEKMPKLRAT STISVGYDNF DVDALTARKI LLMHTPTVLT
110 120 130 140 150
ETVADTLMAL VLSTARRVVE VAERVKAGEW TASIGPDWYG TDVHHKTLGI
160 170 180 190 200
VGMGRIGMAL AQRAHFGFNM PILYNARRHH KEAEERFNAR YCDLDTLLQE
210 220 230 240 250
SDFVCLILPL TDETHHLFGA EQFAKMKSSA IFINAGRGPV VDENALIAAL
260 270 280 290 300
QKGEIHAAGL DVFEQEPLSV DSPLLSMANV VAVPHIGSAT HETRYGMAAC
310 320
AVDNLIDALQ GKVEKNCVNP HVAD
Length:324
Mass (Da):35,396
Last modified:December 15, 1998 - v3
Checksum:i1B21339B1337D255
GO

Sequence cautioni

The sequence AAB18530 differs from that shown. Reason: Frameshift at position 324.Curated
The sequence AAB18530 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18530.1 Frameshift.
U00096 Genomic DNA Translation: AAC76577.2
AP009048 Genomic DNA Translation: BAE77742.1
PIRiC65154
RefSeqiNP_418009.2, NC_000913.3
WP_000805038.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76577; AAC76577; b3553
BAE77742; BAE77742; BAE77742
GeneIDi948074
KEGGiecj:JW5656
eco:b3553
PATRICifig|1411691.4.peg.3161

Similar proteinsi

Entry informationi

Entry nameiGHRB_ECOLI
AccessioniPrimary (citable) accession number: P37666
Secondary accession number(s): Q2M7L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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