UniProtKB - P37651 (GUN_ECOLI)
Protein
Endoglucanase
Gene
bcsZ
Organism
Escherichia coli (strain K12)
Status
Functioni
Hydrolyzes carboxymethylcellulose.
Miscellaneous
The genes bscA, bcsB, bcsZ and bcsC are constitutively transcribed but cellulose synthesis occurs only when DgcC, a putative transmembrane protein regulated by CsgD, is expressed. Cellulose production is abolished in E.coli K12.
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4
: bacterial cellulose biosynthesis Pathwayi
This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 55 | Proton donorBy similarity | 1 | |
Active sitei | 116 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cellulase activity Source: EcoCyc
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12258-MONOMER MetaCyc:EG12258-MONOMER |
UniPathwayi | UPA00694 |
Protein family/group databases
CAZyi | GH8, Glycoside Hydrolase Family 8 |
TCDBi | 4.D.3.1.6, the glycan glucosyl transferase (opgh) family |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase (EC:3.2.1.4)Alternative name(s): Carboxymethylcellulase Short name: CMCase Cellulase Endo-1,4-beta-glucanase |
Gene namesi | Name:bcsZ Synonyms:bcsC, yhjM Ordered Locus Names:b3531, JW3499 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- extracellular region Source: EcoCyc
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000007938 | 22 – 368 | EndoglucanaseAdd BLAST | 347 |
Proteomic databases
PaxDbi | P37651 |
PRIDEi | P37651 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263272, 100 interactors |
IntActi | P37651, 4 interactors |
STRINGi | 511145.b3531 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37651 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 8 (cellulase D) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3405, Bacteria |
HOGENOMi | CLU_037297_0_0_6 |
InParanoidi | P37651 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR002037, Glyco_hydro_8 IPR019834, Glyco_hydro_8_CS |
Pfami | View protein in Pfam PF01270, Glyco_hydro_8, 1 hit |
PRINTSi | PR00735, GLHYDRLASE8 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00812, GLYCOSYL_HYDROL_F8, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P37651-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNVLRSGIVT MLLLAAFSVQ AACTWPAWEQ FKKDYISQEG RVIDPSDARK
60 70 80 90 100
ITTSEGQSYG MFSALAANDR AAFDNILDWT QNNLAQGSLK ERLPAWLWGK
110 120 130 140 150
KENSKWEVLD SNSASDGDVW MAWSLLEAGR LWKEQRYTDI GSALLKRIAR
160 170 180 190 200
EEVVTVPGLG SMLLPGKVGF AEDNSWRFNP SYLPPTLAQY FTRFGAPWTT
210 220 230 240 250
LRETNQRLLL ETAPKGFSPD WVRYEKDKGW QLKAEKTLIS SYDAIRVYMW
260 270 280 290 300
VGMMPDSDPQ KARMLNRFKP MATFTEKNGY PPEKVDVATG KAQGKGPVGF
310 320 330 340 350
SAAMLPFLQN RDAQAVQRQR VADNFPGSDA YYNYVLTLFG QGWDQHRFRF
360
STKGELLPDW GQECANSH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18508.1 U00096 Genomic DNA Translation: AAC76556.1 AP009048 Genomic DNA Translation: BAE77763.1 |
PIRi | S47752 |
RefSeqi | NP_417988.1, NC_000913.3 WP_001311203.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76556; AAC76556; b3531 BAE77763; BAE77763; BAE77763 |
GeneIDi | 948046 |
KEGGi | ecj:JW3499 eco:b3531 |
PATRICi | fig|511145.12.peg.3642 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18508.1 U00096 Genomic DNA Translation: AAC76556.1 AP009048 Genomic DNA Translation: BAE77763.1 |
PIRi | S47752 |
RefSeqi | NP_417988.1, NC_000913.3 WP_001311203.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3QXF | X-ray | 1.85 | A/B/C/D | 22-368 | [»] | |
3QXQ | X-ray | 2.20 | A/B/C/D | 22-368 | [»] | |
SMRi | P37651 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263272, 100 interactors |
IntActi | P37651, 4 interactors |
STRINGi | 511145.b3531 |
Protein family/group databases
CAZyi | GH8, Glycoside Hydrolase Family 8 |
TCDBi | 4.D.3.1.6, the glycan glucosyl transferase (opgh) family |
Proteomic databases
PaxDbi | P37651 |
PRIDEi | P37651 |
Genome annotation databases
EnsemblBacteriai | AAC76556; AAC76556; b3531 BAE77763; BAE77763; BAE77763 |
GeneIDi | 948046 |
KEGGi | ecj:JW3499 eco:b3531 |
PATRICi | fig|511145.12.peg.3642 |
Organism-specific databases
EchoBASEi | EB2167 |
Phylogenomic databases
eggNOGi | COG3405, Bacteria |
HOGENOMi | CLU_037297_0_0_6 |
InParanoidi | P37651 |
Enzyme and pathway databases
UniPathwayi | UPA00694 |
BioCyci | EcoCyc:EG12258-MONOMER MetaCyc:EG12258-MONOMER |
Miscellaneous databases
PROi | PR:P37651 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR012341, 6hp_glycosidase-like_sf IPR002037, Glyco_hydro_8 IPR019834, Glyco_hydro_8_CS |
Pfami | View protein in Pfam PF01270, Glyco_hydro_8, 1 hit |
PRINTSi | PR00735, GLHYDRLASE8 |
SUPFAMi | SSF48208, SSF48208, 1 hit |
PROSITEi | View protein in PROSITE PS00812, GLYCOSYL_HYDROL_F8, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GUN_ECOLI | |
Accessioni | P37651Primary (citable) accession number: P37651 Secondary accession number(s): Q2M7J3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries