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UniProtKB - P37649 (PDEK_ECOLI)
Protein
Probable cyclic di-GMP phosphodiesterase PdeK
Gene
pdeK
Organism
Escherichia coli (strain K12)
Status
Functioni
Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG.
By similarityCatalytic activityi
- EC:3.1.4.52By similarity
GO - Molecular functioni
- cyclic-guanylate-specific phosphodiesterase activity Source: EcoCyc
GO - Biological processi
- signal transduction Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Ligand | c-di-GMP |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12256-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdeK1 Publication Synonyms:yhjK Ordered Locus Names:b3529, JW5943 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 12 – 32 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 158 – 178 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169574 | 1 – 662 | Probable cyclic di-GMP phosphodiesterase PdeKAdd BLAST | 662 |
Proteomic databases
jPOSTi | P37649 |
PaxDbi | P37649 |
PRIDEi | P37649 |
Expressioni
Inductioni
Expressed at 28 degrees Celsius in stationary phase.1 Publication
Interactioni
Binary interactionsi
P37649
With | #Exp. | IntAct |
---|---|---|
hscC [P77319] | 3 | EBI-562146,EBI-562084 |
Protein-protein interaction databases
BioGRIDi | 4262160, 7 interactors |
DIPi | DIP-12384N |
IntActi | P37649, 1 interactor |
STRINGi | 511145.b3529 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 180 – 236 | HAMPPROSITE-ProRule annotationAdd BLAST | 57 | |
Domaini | 268 – 400 | GGDEFPROSITE-ProRule annotationAdd BLAST | 133 | |
Domaini | 409 – 662 | EALPROSITE-ProRule annotationAdd BLAST | 254 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2199, Bacteria COG2200, Bacteria |
HOGENOMi | CLU_000445_70_46_6 |
OMAi | DREMGMQ |
PhylomeDBi | P37649 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR001633, EAL_dom IPR035919, EAL_sf IPR033419, GAPES3 IPR000160, GGDEF_dom IPR003660, HAMP_dom IPR029787, Nucleotide_cyclase IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00563, EAL, 1 hit PF17154, GAPES3, 1 hit PF00990, GGDEF, 1 hit PF00672, HAMP, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit SM00267, GGDEF, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit SSF55073, SSF55073, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit PS50887, GGDEF, 1 hit PS50885, HAMP, 1 hit |
i Sequence
Sequence statusi: Complete.
P37649-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVSRSLTIK QMAMVAAVVL VFVFIFCTVL LFHLVQQNRY NTATQLESIA
60 70 80 90 100
RSVREPLSSA ILKGDIPEAE AILASIKPAG VVSRADVVLP NQFQALRKSF
110 120 130 140 150
IPERPVPVMV TRLFELPVQI SLGVYSLERP ANPQPIAYLV LQADSFRMYK
160 170 180 190 200
FVMSTLSTLV TIYLLLSLIL TVAISWCINR LILHPLRNIA RELNAIPAKE
210 220 230 240 250
LVGHQLALPR LHQDDEIGML VRSYNLNQQL LQRHYEEQNE NAMRFPVSDL
260 270 280 290 300
PNKALLMEML EQVVARKQTT ALMIITCETL RDTAGVLKEA QREILLLTLV
310 320 330 340 350
EKLKSVLSPR MILAQISGYD FAVIANGVQE PWHAITLGQQ VLTIMSERLP
360 370 380 390 400
IERIQLRPHC SIGVAMFYGD LTAEQLYSRA ISAAFTARHK GKNQIQFFDP
410 420 430 440 450
QQMEAAQKRL TEESDILNAL ENHQFAIWLQ PQVEMTSGKL VSAEVLLRIQ
460 470 480 490 500
QPDGSWDLPD GLIDRIECCG LMVTVGHWVL EESCRLLAAW QERGIMLPLS
510 520 530 540 550
VNLSALQLMH PNMVADMLEL LTRYRIQPGT LILEVTESRR IDDPHAAVAI
560 570 580 590 600
LRPLRNAGVR VALDDFGMGY AGLRQLQHMK SLPIDVLKID KMFVEGLPGD
610 620 630 640 650
SSMIAAIIML AQSLNLQMIA EGVETEAQRD WLAKAGVGIA QGFLFARPLP
660
IEIFEESYLE EK
Sequence cautioni
The sequence AAB18506 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAE77765 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18506.1 Different initiation. U00096 Genomic DNA Translation: AAC76554.4 AP009048 Genomic DNA Translation: BAE77765.1 Different initiation. |
PIRi | S47750 |
RefSeqi | NP_417986.4, NC_000913.3 WP_001266293.1, NZ_SSZK01000039.1 |
Genome annotation databases
EnsemblBacteriai | AAC76554; AAC76554; b3529 BAE77765; BAE77765; BAE77765 |
GeneIDi | 948048 |
KEGGi | ecj:JW5943 eco:b3529 |
PATRICi | fig|511145.12.peg.3640 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18506.1 Different initiation. U00096 Genomic DNA Translation: AAC76554.4 AP009048 Genomic DNA Translation: BAE77765.1 Different initiation. |
PIRi | S47750 |
RefSeqi | NP_417986.4, NC_000913.3 WP_001266293.1, NZ_SSZK01000039.1 |
3D structure databases
AlphaFoldDBi | P37649 |
SMRi | P37649 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262160, 7 interactors |
DIPi | DIP-12384N |
IntActi | P37649, 1 interactor |
STRINGi | 511145.b3529 |
Proteomic databases
jPOSTi | P37649 |
PaxDbi | P37649 |
PRIDEi | P37649 |
Genome annotation databases
EnsemblBacteriai | AAC76554; AAC76554; b3529 BAE77765; BAE77765; BAE77765 |
GeneIDi | 948048 |
KEGGi | ecj:JW5943 eco:b3529 |
PATRICi | fig|511145.12.peg.3640 |
Organism-specific databases
EchoBASEi | EB2165 |
Phylogenomic databases
eggNOGi | COG2199, Bacteria COG2200, Bacteria |
HOGENOMi | CLU_000445_70_46_6 |
OMAi | DREMGMQ |
PhylomeDBi | P37649 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12256-MONOMER |
Miscellaneous databases
PROi | PR:P37649 |
Family and domain databases
CDDi | cd01948, EAL, 1 hit |
Gene3Di | 3.20.20.450, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR001633, EAL_dom IPR035919, EAL_sf IPR033419, GAPES3 IPR000160, GGDEF_dom IPR003660, HAMP_dom IPR029787, Nucleotide_cyclase IPR043128, Rev_trsase/Diguanyl_cyclase |
Pfami | View protein in Pfam PF00563, EAL, 1 hit PF17154, GAPES3, 1 hit PF00990, GGDEF, 1 hit PF00672, HAMP, 1 hit |
SMARTi | View protein in SMART SM00052, EAL, 1 hit SM00267, GGDEF, 1 hit |
SUPFAMi | SSF141868, SSF141868, 1 hit SSF55073, SSF55073, 1 hit |
PROSITEi | View protein in PROSITE PS50883, EAL, 1 hit PS50887, GGDEF, 1 hit PS50885, HAMP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PDEK_ECOLI | |
Accessioni | P37649Primary (citable) accession number: P37649 Secondary accession number(s): Q2M7J1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | June 15, 2010 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |