UniProtKB - P37647 (KDGK_ECOLI)
Protein
2-dehydro-3-deoxygluconokinase
Gene
kdgK
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG).1 Publication
Catalytic activityi
- 2-dehydro-3-deoxy-D-gluconate + ATP = 2-dehydro-3-deoxy-6-phospho-D-gluconate + ADP + H+1 PublicationEC:2.7.1.451 Publication
Kineticsi
- KM=1 µM for ATP1 Publication
- KM=1 µM for KDG1 Publication
pH dependencei
Optimum pH is around 6.1 Publication
: 2-dehydro-3-deoxy-D-gluconate degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- 2-dehydro-3-deoxygluconokinase (kdgK)
- KHG/KDPG aldolase (eda)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate, the pathway 2-dehydro-3-deoxy-D-gluconate degradation and in Carbohydrate acid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 88 | SubstrateBy similarity | 1 | |
Binding sitei | 170 | SubstrateBy similarity | 1 | |
Binding sitei | 197 | ATPBy similarity | 1 | |
Active sitei | 264 | Proton acceptorBy similarity | 1 | |
Binding sitei | 264 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 168 – 170 | ATPBy similarity | 3 | |
Nucleotide bindingi | 228 – 234 | ATPBy similarity | 7 | |
Nucleotide bindingi | 261 – 264 | ATPBy similarity | 4 |
GO - Molecular functioni
- 2-dehydro-3-deoxygluconokinase activity Source: EcoCyc
- ATP binding Source: UniProtKB-KW
- carbohydrate kinase activity Source: GO_Central
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- D-galacturonate catabolic process Source: EcoCyc
- D-glucuronate catabolic process Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:DEOXYGLUCONOKIN-MONOMER ECOL316407:JW5668-MONOMER MetaCyc:DEOXYGLUCONOKIN-MONOMER |
UniPathwayi | UPA00856;UER00828 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-dehydro-3-deoxygluconokinase (EC:2.7.1.45)Alternative name(s): 2-keto-3-deoxygluconokinase 3-deoxy-2-oxo-D-gluconate kinase KDG kinase |
Gene namesi | Name:kdgK Synonyms:yhjI Ordered Locus Names:b3526, JW5668 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080085 | 1 – 309 | 2-dehydro-3-deoxygluconokinaseAdd BLAST | 309 |
Proteomic databases
jPOSTi | P37647 |
PaxDbi | P37647 |
PRIDEi | P37647 |
Interactioni
Protein-protein interaction databases
BioGridi | 4262528, 7 interactors 852349, 2 interactors |
DIPi | DIP-10056N |
IntActi | P37647, 5 interactors |
STRINGi | 511145.b3526 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 102 – 104 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the carbohydrate kinase PfkB family.Curated
Phylogenomic databases
eggNOGi | ENOG4107SS6 Bacteria COG0524 LUCA |
HOGENOMi | HOG000235952 |
InParanoidi | P37647 |
KOi | K00874 |
PhylomeDBi | P37647 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR002173 Carboh/pur_kinase_PfkB_CS IPR011611 PfkB_dom IPR029056 Ribokinase-like |
Pfami | View protein in Pfam PF00294 PfkB, 1 hit |
SUPFAMi | SSF53613 SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00584 PFKB_KINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P37647-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKKIAVIGE CMIELSEKGA DVKRGFGGDT LNTSVYIARQ VDPAALTVHY
60 70 80 90 100
VTALGTDSFS QQMLDAWHGE NVDTSLTQRM ENRLPGLYYI ETDSTGERTF
110 120 130 140 150
YYWRNEAAAK FWLESEQSAA ICEELANFDY LYLSGISLAI LSPTSREKLL
160 170 180 190 200
SLLRECRANG GKVIFDNNYR PRLWASKEET QQVYQQMLEC TDIAFLTLDD
210 220 230 240 250
EDALWGQQPV EDVIARTHNA GVKEVVVKRG ADSCLVSIAG EGLVDVPAVK
260 270 280 290 300
LPKEKVIDTT AAGDSFSAGY LAVRLTGGSA EDAAKRGHLT ASTVIQYRGA
IIPREAMPA
Sequence cautioni
The sequence AAB18503 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18503.1 Different initiation. U00096 Genomic DNA Translation: AAC76551.2 AP009048 Genomic DNA Translation: BAE77768.1 |
PIRi | S47747 |
RefSeqi | NP_417983.2, NC_000913.3 WP_000037562.1, NZ_SSZK01000039.1 |
Genome annotation databases
EnsemblBacteriai | AAC76551; AAC76551; b3526 BAE77768; BAE77768; BAE77768 |
GeneIDi | 948041 |
KEGGi | ecj:JW5668 eco:b3526 |
PATRICi | fig|511145.12.peg.3636 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00039 Genomic DNA Translation: AAB18503.1 Different initiation. U00096 Genomic DNA Translation: AAC76551.2 AP009048 Genomic DNA Translation: BAE77768.1 |
PIRi | S47747 |
RefSeqi | NP_417983.2, NC_000913.3 WP_000037562.1, NZ_SSZK01000039.1 |
3D structure databases
SMRi | P37647 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 4262528, 7 interactors 852349, 2 interactors |
DIPi | DIP-10056N |
IntActi | P37647, 5 interactors |
STRINGi | 511145.b3526 |
Proteomic databases
jPOSTi | P37647 |
PaxDbi | P37647 |
PRIDEi | P37647 |
Genome annotation databases
EnsemblBacteriai | AAC76551; AAC76551; b3526 BAE77768; BAE77768; BAE77768 |
GeneIDi | 948041 |
KEGGi | ecj:JW5668 eco:b3526 |
PATRICi | fig|511145.12.peg.3636 |
Organism-specific databases
EchoBASEi | EB2163 |
Phylogenomic databases
eggNOGi | ENOG4107SS6 Bacteria COG0524 LUCA |
HOGENOMi | HOG000235952 |
InParanoidi | P37647 |
KOi | K00874 |
PhylomeDBi | P37647 |
Enzyme and pathway databases
UniPathwayi | UPA00856;UER00828 |
BioCyci | EcoCyc:DEOXYGLUCONOKIN-MONOMER ECOL316407:JW5668-MONOMER MetaCyc:DEOXYGLUCONOKIN-MONOMER |
Miscellaneous databases
PROi | PR:P37647 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR002173 Carboh/pur_kinase_PfkB_CS IPR011611 PfkB_dom IPR029056 Ribokinase-like |
Pfami | View protein in Pfam PF00294 PfkB, 1 hit |
SUPFAMi | SSF53613 SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00584 PFKB_KINASES_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDGK_ECOLI | |
Accessioni | P37647Primary (citable) accession number: P37647 Secondary accession number(s): P78117, Q2M7I8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | November 13, 2019 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PATHWAY comments
Index of metabolic and biosynthesis pathways