kdgK

Functionⁱ

Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG).1 Publication

Catalytic activityⁱ

ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate.1 Publication

Kineticsⁱ

K_M=
1 µM for ATP
1 Publication
Manual assertion based on experiment inⁱ
- Ref.4
  "Study of the common degradative pathway of hexuronates in Escherichia coli K 12. Purification, properties and individuality of 2-keto-3-deoxy-D-gluconnokinase."
  Pouyssegur J., Stoeber F.
  Biochimie 53:771-781(1971) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
K_M=
1 µM for KDG
1 Publication
Manual assertion based on experiment inⁱ
- Ref.4
  "Study of the common degradative pathway of hexuronates in Escherichia coli K 12. Purification, properties and individuality of 2-keto-3-deoxy-D-gluconnokinase."
  Pouyssegur J., Stoeber F.
  Biochimie 53:771-781(1971) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

pH dependenceⁱ

Optimum pH is around 6.1 Publication

Pathwayⁱ: 2-dehydro-3-deoxy-D-gluconate degradation

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

2-dehydro-3-deoxygluconokinase (kdgK)
KHG/KDPG aldolase (eda)

This subpathway is part of the pathway 2-dehydro-3-deoxy-D-gluconate degradation, which is itself part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate, the pathway 2-dehydro-3-deoxy-D-gluconate degradation and in Carbohydrate acid metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	88	SubstrateBy similarity	1
Binding siteⁱ	170	SubstrateBy similarity	1
Binding siteⁱ	197	ATPBy similarity	1
Active siteⁱ	264	Proton acceptorBy similarity	1
Binding siteⁱ	264	SubstrateBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	168 – 170	ATPBy similarity	3
Nucleotide bindingⁱ	228 – 234	ATPBy similarity	7
Nucleotide bindingⁱ	261 – 264	ATPBy similarity	4

GO - Molecular functionⁱ

2-dehydro-3-deoxygluconokinase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 13813474
- 4944816
ATP binding Source: UniProtKB-KW
carbohydrate kinase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Biological process	Carbohydrate metabolism
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:DEOXYGLUCONOKIN-MONOMER MetaCyc:DEOXYGLUCONOKIN-MONOMER
UniPathwayⁱ	UPA00856;UER00828

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 2-dehydro-3-deoxygluconokinase (EC:2.7.1.45) Alternative name(s): 2-keto-3-deoxygluconokinase 3-deoxy-2-oxo-D-gluconate kinase KDG kinase
Gene namesⁱ	Name:kdgK Synonyms:yhjI Ordered Locus Names:b3526, JW5668
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12253 kdgK

EcoGeneⁱ

EG12253 kdgK

Subcellular locationⁱ

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323

View the complete GO annotation on QuickGO ...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000080085	1 – 309	2-dehydro-3-deoxygluconokinaseAdd BLAST		309

Proteomic databases

PaxDbⁱ	P37647
PRIDEⁱ	P37647

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4262528, 7 interactors
Database of interacting proteins More...DIPⁱ	DIP-10056N
IntActⁱ	P37647, 5 interactors
STRINGⁱ	316385.ECDH10B_3703

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P37647
SMRⁱ	P37647
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	102 – 104	Substrate bindingBy similarity		3

Sequence similaritiesⁱ

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4107SS6 Bacteria COG0524 LUCA
HOGENOMⁱ	HOG000235952
InParanoidⁱ	P37647
KEGG Orthology (KO) More...KOⁱ	K00874
PhylomeDBⁱ	P37647

Family and domain databases

Gene3Dⁱ	3.40.1190.20, 1 hit
InterProⁱ	View protein in InterPro IPR002173 Carboh/pur_kinase_PfkB_CS IPR011611 PfkB_dom IPR029056 Ribokinase-like
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00294 PfkB, 1 hit
SUPFAMⁱ	SSF53613 SSF53613, 1 hit
PROSITEⁱ	View protein in PROSITE PS00584 PFKB_KINASES_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P37647-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKKIAVIGE CMIELSEKGA DVKRGFGGDT LNTSVYIARQ VDPAALTVHY 
        60         70         80         90        100
VTALGTDSFS QQMLDAWHGE NVDTSLTQRM ENRLPGLYYI ETDSTGERTF 
       110        120        130        140        150
YYWRNEAAAK FWLESEQSAA ICEELANFDY LYLSGISLAI LSPTSREKLL 
       160        170        180        190        200
SLLRECRANG GKVIFDNNYR PRLWASKEET QQVYQQMLEC TDIAFLTLDD 
       210        220        230        240        250
EDALWGQQPV EDVIARTHNA GVKEVVVKRG ADSCLVSIAG EGLVDVPAVK 
       260        270        280        290        300
LPKEKVIDTT AAGDSFSAGY LAVRLTGGSA EDAAKRGHLT ASTVIQYRGA 

IIPREAMPA

Length:309

Mass (Da):33,962

Last modified:October 1, 1994 - v1

Checksum:ⁱ53FCD14696BCFA33

GO

Sequence cautionⁱ

The sequence AAB18503 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00039 Genomic DNA Translation: AAB18503.1 Different initiation. U00096 Genomic DNA Translation: AAC76551.2 AP009048 Genomic DNA Translation: BAE77768.1
PIRⁱ	S47747
NCBI Reference Sequences More...RefSeqⁱ	NP_417983.2, NC_000913.3 WP_000037562.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76551; AAC76551; b3526 BAE77768; BAE77768; BAE77768
GeneIDⁱ	948041
KEGGⁱ	ecj:JW5668 eco:b3526
PATRICⁱ	fig\|511145.12.peg.3636

Protein	Similar proteins		Species	Score	Length	Source
P37647	Ketodeoxygluconokinase		Escherichia coli H736		382	UniRef100_P37647
Ketodeoxygluconokinase		Escherichia coli H386		382
Ketodeoxygluconokinase		Escherichia coli M605		382
PfkB domain containing protein		TRITR		309
Ketodeoxygluconokinase		Shigella sp.		309
+43

Protein	Similar proteins		Species	Score	Length	Source
P37647	2-dehydro-3-deoxygluconokinase		ECOLW		309	UniRef90_P37647
Ketodeoxygluconokinase		Escherichia coli H736		382
Ketodeoxygluconokinase		Escherichia coli H386		382
Ketodeoxygluconokinase		Escherichia coli M605		382
PfkB domain containing protein		TRITR		309
+161

Protein	Similar proteins		Species	Score	Length	Source
P37647	2-dehydro-3-deoxygluconokinase		ECOLW		309	UniRef50_P37647
Ketodeoxygluconokinase		Escherichia coli H736		382
Ketodeoxygluconokinase		Escherichia coli H386		382
Ketodeoxygluconokinase		Escherichia coli M605		382
PfkB domain containing protein		TRITR		309
+229

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00039 Genomic DNA Translation: AAB18503.1 Different initiation. U00096 Genomic DNA Translation: AAC76551.2 AP009048 Genomic DNA Translation: BAE77768.1
PIRⁱ	S47747
RefSeqⁱ	NP_417983.2, NC_000913.3 WP_000037562.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P37647
SMRⁱ	P37647
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262528, 7 interactors
DIPⁱ	DIP-10056N
IntActⁱ	P37647, 5 interactors
STRINGⁱ	316385.ECDH10B_3703

Proteomic databases

PaxDbⁱ	P37647
PRIDEⁱ	P37647

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76551; AAC76551; b3526 BAE77768; BAE77768; BAE77768
GeneIDⁱ	948041
KEGGⁱ	ecj:JW5668 eco:b3526
PATRICⁱ	fig\|511145.12.peg.3636

Organism-specific databases

EchoBASEⁱ	EB2163
EcoGeneⁱ	EG12253 kdgK

Phylogenomic databases

eggNOGⁱ	ENOG4107SS6 Bacteria COG0524 LUCA
HOGENOMⁱ	HOG000235952
InParanoidⁱ	P37647
KOⁱ	K00874
PhylomeDBⁱ	P37647

Enzyme and pathway databases

UniPathwayⁱ	UPA00856;UER00828
BioCycⁱ	EcoCyc:DEOXYGLUCONOKIN-MONOMER MetaCyc:DEOXYGLUCONOKIN-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P37647

PROⁱ

PR:P37647

Family and domain databases

Gene3Dⁱ	3.40.1190.20, 1 hit
InterProⁱ	View protein in InterPro IPR002173 Carboh/pur_kinase_PfkB_CS IPR011611 PfkB_dom IPR029056 Ribokinase-like
Pfamⁱ	View protein in Pfam PF00294 PfkB, 1 hit
SUPFAMⁱ	SSF53613 SSF53613, 1 hit
PROSITEⁱ	View protein in PROSITE PS00584 PFKB_KINASES_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KDGK_ECOLI
Accessionⁱ	P37647Primary (citable) accession number: P37647 Secondary accession number(s): P78117, Q2M7I8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
	Last sequence update:	October 1, 1994
	Last modified:	November 7, 2018
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P37647 (KDGK_ECOLI)

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2-dehydro-3-deoxygluconokinase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

pH dependencei

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: 2-dehydro-3-deoxy-D-gluconate degradation

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

pH dependenceⁱ

Pathwayⁱ: 2-dehydro-3-deoxy-D-gluconate degradation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ