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Protein

Ribosome-associated ATPase

Gene

rbbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits an intrinsic ATPase activity that is stimulated by both 70S ribosomes and 30S ribosomal subunits. Could be involved in protein-chain elongation and in release of deacyl-tRNA from ribosomes after peptide bond synthesis. Stimulates the synthesis of polyphenylalanine in vitro.3 Publications

Activity regulationi

Hygromycin B inhibits RbbA ATPase activity and releases RbbA from 70S ribosomes.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi309 – 316ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YHIH-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-associated ATPaseCurated
Alternative name(s):
Ribosomal bound ATPase1 Publication
Gene namesi
Name:rbbA1 Publication
Synonyms:yhiG, yhiH
Ordered Locus Names:b3486, JW5676
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12223 rbbA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei563 – 583HelicalSequence analysisAdd BLAST21
Transmembranei716 – 736HelicalSequence analysisAdd BLAST21
Transmembranei769 – 789HelicalSequence analysisAdd BLAST21
Transmembranei796 – 816HelicalSequence analysisAdd BLAST21
Transmembranei824 – 844HelicalSequence analysisAdd BLAST21
Transmembranei886 – 906HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion mutant is slightly elongated at both 15 and 32 degrees Celsius and displays a significant loss of translation fidelity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000931901 – 911Ribosome-associated ATPaseAdd BLAST911

Proteomic databases

PaxDbiP37624
PRIDEiP37624

Interactioni

Subunit structurei

Interacts with both 70S ribosomes and 30S ribosomal subunits, and with 16S RNA (PubMed:10607404, PubMed:11168361, PubMed:16495476). Binds EF-Tu (PubMed:11168361). Co-purifies with the inner membrane protein YhjD (PubMed:21556145).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
dhaKP760152EBI-544500,EBI-544485

Protein-protein interaction databases

BioGridi4259649, 268 interactors
DIPiDIP-12363N
IntActiP37624, 1 interactor
STRINGi316385.ECDH10B_3661

Structurei

3D structure databases

ProteinModelPortaliP37624
SMRiP37624
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 248ABC transporter 1PROSITE-ProRule annotationAdd BLAST236
Domaini277 – 507ABC transporter 2PROSITE-ProRule annotationAdd BLAST231
Domaini683 – 909ABC transmembrane type-2PROSITE-ProRule annotationAdd BLAST227

Sequence similaritiesi

In the C-terminal section; belongs to the ABC-2 integral membrane protein family.Curated
Belongs to the ABC transporter superfamily.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJ1 Bacteria
COG0842 LUCA
COG1131 LUCA
HOGENOMiHOG000014886
InParanoidiP37624
KOiK13926
OMAiFTENFTD
PhylomeDBiP37624

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR000412 ABC_2_transport
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51012 ABC_TM2, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 2 hits

Sequencei

Sequence statusi: Complete.

P37624-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTHLELVPVP PVAQLAGVSQ HYGKTVALNN ITLDIPARCM VGLIGPDGVG
60 70 80 90 100
KSSLLSLISG ARVIEQGNVM VLGGDMRDPK HRRDVCPRIA WMPQGLGKNL
110 120 130 140 150
YHTLSVYENV DFFARLFGHD KAEREVRINE LLTSTGLAPF RDRPAGKLSG
160 170 180 190 200
GMKQKLGLCC ALIHDPELLI LDEPTTGVDP LSRSQFWDLI DSIRQRQSNM
210 220 230 240 250
SVLVATAYME EAERFDWLVA MNAGEVLATG SAEELRQQTQ SATLEEAFIN
260 270 280 290 300
LLPQAQRQAH QAVVIPPYQP ENAEIAIEAR DLTMRFGSFV AVDHVNFRIP
310 320 330 340 350
RGEIFGFLGS NGCGKSTTMK MLTGLLPASE GEAWLFGQPV DPKDIDTRRR
360 370 380 390 400
VGYMSQAFSL YNELTVRQNL ELHARLFHIP EAEIPARVAE MSERFKLNDV
410 420 430 440 450
EDILPESLPL GIRQRLSLAV AVIHRPEMLI LDEPTSGVDP VARDMFWQLM
460 470 480 490 500
VDLSRQDKVT IFISTHFMNE AERCDRISLM HAGKVLASGT PQELVEKRGA
510 520 530 540 550
ASLEEAFIAY LQEAAGQSNE AEAPPVVHDT THAPRQGFSL RRLFSYSRRE
560 570 580 590 600
ALELRRDPVR STLALMGTVI LMLIMGYGIS MDVENLRFAV LDRDQTVSSQ
610 620 630 640 650
AWTLNLSGSR YFIEQPPLTS YDELDRRMRA GDITVAIEIP PNFGRDIARG
660 670 680 690 700
TPVELGVWID GAMPSRAETV KGYVQAMHQS WLQDVASRQS TPASQSGLMN
710 720 730 740 750
IETRYRYNPD VKSLPAIVPA VIPLLLMMIP SMLSALSVVR EKELGSIINL
760 770 780 790 800
YVTPTTRSEF LLGKQLPYIA LGMLNFFLLC GLSVFVFGVP HKGSFLTLTL
810 820 830 840 850
AALLYIIIAT GMGLLISTFM KSQIAAIFGT AIITLIPATQ FSGMIDPVAS
860 870 880 890 900
LEGPGRWIGE VYPTSHFLTI ARGTFSKALD LTDLWQLFIP LLIAIPLVMG
910
LSILLLKKQE G
Length:911
Mass (Da):100,785
Last modified:October 11, 2004 - v3
Checksum:i683E1EBB62E6B69E
GO

Sequence cautioni

The sequence AAB18462 differs from that shown. Reason: Frameshift at positions 10 and 241.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18461.1 Frameshift.
U00039 Genomic DNA Translation: AAB18462.1 Frameshift.
U00096 Genomic DNA Translation: AAT48187.1
AP009048 Genomic DNA Translation: BAE77807.1
PIRiA65146
RefSeqiWP_000149156.1, NZ_LN832404.1
YP_026225.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48187; AAT48187; b3486
BAE77807; BAE77807; BAE77807
GeneIDi947998
KEGGiecj:JW5676
eco:b3486
PATRICifig|1411691.4.peg.3238

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18461.1 Frameshift.
U00039 Genomic DNA Translation: AAB18462.1 Frameshift.
U00096 Genomic DNA Translation: AAT48187.1
AP009048 Genomic DNA Translation: BAE77807.1
PIRiA65146
RefSeqiWP_000149156.1, NZ_LN832404.1
YP_026225.1, NC_000913.3

3D structure databases

ProteinModelPortaliP37624
SMRiP37624
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259649, 268 interactors
DIPiDIP-12363N
IntActiP37624, 1 interactor
STRINGi316385.ECDH10B_3661

Proteomic databases

PaxDbiP37624
PRIDEiP37624

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48187; AAT48187; b3486
BAE77807; BAE77807; BAE77807
GeneIDi947998
KEGGiecj:JW5676
eco:b3486
PATRICifig|1411691.4.peg.3238

Organism-specific databases

EchoBASEiEB2136
EcoGeneiEG12223 rbbA

Phylogenomic databases

eggNOGiENOG4105CJ1 Bacteria
COG0842 LUCA
COG1131 LUCA
HOGENOMiHOG000014886
InParanoidiP37624
KOiK13926
OMAiFTENFTD
PhylomeDBiP37624

Enzyme and pathway databases

BioCyciEcoCyc:YHIH-MONOMER

Miscellaneous databases

PROiPR:P37624

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR000412 ABC_2_transport
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51012 ABC_TM2, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiRBBA_ECOLI
AccessioniPrimary (citable) accession number: P37624
Secondary accession number(s): P37625, Q2M7E9, Q6BF27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 11, 2004
Last modified: September 12, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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