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Protein

HTH-type transcriptional regulator GlnR

Gene

glnR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription repressor that represses many genes including ureABC and tnrA, during nitrogen excess (PubMed:2573733, PubMed:1677938, PubMed:10231480, PubMed:18331450). On the contrary of the MerR members, which require longer DNA sites for high-affinity binding, GlnR requires a DNA sequence of 17 nucleotides as minimal binding site (PubMed:10231480, PubMed:25691471).5 Publications

Miscellaneous

The amino acid sequences of the N-terminal DNA binding domains of TnrA and GlnR are highly similar, and both proteins bind to DNA sequences with a common consensus sequence. In contrast, the C-terminal signal transduction domains of TnrA and GlnR have no homology.1 Publication

Activity regulationi

Under conditions of nitrogen excess, the DNA binding activity of GlnR is activated by a transient interaction with feedback-inhibited GlnA (PubMed:18331450). Under conditions of nitrogen-limited, GlnR is autoinhibited by its C-terminal region (PubMed:25691471).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi14 – 33H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciBSUB:BSU17450-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator GlnR1 Publication
Gene namesi
Name:glnR1 Publication
Ordered Locus Names:BSU17450
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

In vivo, this mutant constitutively represses glnR expression. In vitro, this mutant binds DNA more tightly than wild-type and this binding is not activated by feedback-inhibited GlnA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000981221 – 135HTH-type transcriptional regulator GlnRAdd BLAST135

Proteomic databases

PaxDbiP37582
PRIDEiP37582

Expressioni

Inductioni

Under conditions of nitrogen-limited growth, repressed by TnrA.3 Publications

Interactioni

Subunit structurei

Homodimer under conditions of nitrogen excess (PubMed:1677938, PubMed:18331450, PubMed:25691471). Monomer under conditions of nitrogen-limited (PubMed:25691471). Interacts with feedback-inhibited GlnA in order to stabilizes GlnR-DNA complex (PubMed:1677938, PubMed:18331450, PubMed:25691471).3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-29671N
IntActiP37582, 1 interactor
STRINGi224308.Bsubs1_010100009601

Structurei

Secondary structure

1135
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP37582
SMRiP37582
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 79HTH merR-typePROSITE-ProRule annotationAdd BLAST69

Phylogenomic databases

eggNOGiENOG4105KV4 Bacteria
COG0789 LUCA
HOGENOMiHOG000266082
InParanoidiP37582
KOiK03713
OMAiMYSLNDV
PhylomeDBiP37582

Family and domain databases

InterProiView protein in InterPro
IPR009061 DNA-bd_dom_put_sf
IPR000551 MerR-type_HTH_dom
PfamiView protein in Pfam
PF13411 MerR_1, 1 hit
SMARTiView protein in SMART
SM00422 HTH_MERR, 1 hit
SUPFAMiSSF46955 SSF46955, 1 hit
PROSITEiView protein in PROSITE
PS00552 HTH_MERR_1, 1 hit
PS50937 HTH_MERR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P37582-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDNIRRSMP LFPIGIVMQL TELSARQIRY YEENGLIFPA RSEGNRRLFS
60 70 80 90 100
FHDVDKLLEI KHLIEQGVNM AGIKQILAKA EAEPEQKQNE KTKKPMKHDL
110 120 130
SDDELRQLLK NELMQAGRFQ RGNTFRQGDM SRFFH
Length:135
Mass (Da):15,832
Last modified:October 1, 1994 - v1
Checksum:i5FF14F3BB2B1F1D7
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti96M → V in strain: PCI 219. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22811 Genomic DNA Translation: AAA83375.1
D00854 Genomic DNA Translation: BAA00729.1
U66480 Genomic DNA Translation: AAB41079.1
AL009126 Genomic DNA Translation: CAB13629.1
PIRiJT0393
RefSeqiNP_389627.1, NC_000964.3
WP_003238341.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13629; CAB13629; BSU17450
GeneIDi940070
KEGGibsu:BSU17450
PATRICifig|224308.179.peg.1893

Similar proteinsi

Entry informationi

Entry nameiGLNR_BACSU
AccessioniPrimary (citable) accession number: P37582
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 12, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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