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UniProtKB - P37563 (TILS_BACSU)
Protein
tRNA(Ile)-lysidine synthase
Gene
tilS
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
1 PublicationCatalytic activityi
- ATP + cytidine34 in tRNAIle2 + L-lysine = AMP + diphosphate + H+ + lysidine34 in tRNAIle21 PublicationEC:6.3.4.191 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 25 – 30 | ATPSequence analysis | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-UniRule
GO - Biological processi
- tRNA modification Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | tRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU00670-MONOMER |
BRENDAi | 6.3.4.19, 658 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA(Ile)-lysidine synthase (EC:6.3.4.19)Alternative name(s): tRNA(Ile)-2-lysyl-cytidine synthase tRNA(Ile)-lysidine synthetase |
Gene namesi | Name:tilS Synonyms:yacA Ordered Locus Names:BSU00670 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000181650 | 1 – 472 | tRNA(Ile)-lysidine synthaseAdd BLAST | 472 |
Proteomic databases
PaxDbi | P37563 |
PRIDEi | P37563 |
Interactioni
Protein-protein interaction databases
IntActi | P37563, 2 interactors |
STRINGi | 224308.BSU00670 |
Family & Domainsi
Domaini
The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.
Sequence similaritiesi
Belongs to the tRNA(Ile)-lysidine synthase family.Curated
Phylogenomic databases
eggNOGi | COG0037, Bacteria |
InParanoidi | P37563 |
OMAi | QQYGMNA |
PhylomeDBi | P37563 |
Family and domain databases
CDDi | cd01992, PP-ATPase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01161, tRNA_Ile_lys_synt, 1 hit |
InterProi | View protein in InterPro IPR012796, Lysidine-tRNA-synth_C IPR014729, Rossmann-like_a/b/a_fold IPR011063, TilS/TtcA_N IPR012094, tRNA_Ile_lys_synt IPR012795, tRNA_Ile_lys_synt_N IPR015262, tRNA_Ile_lys_synt_subst-bd |
PANTHERi | PTHR43033, PTHR43033, 1 hit |
Pfami | View protein in Pfam PF01171, ATP_bind_3, 1 hit PF09179, TilS, 1 hit PF11734, TilS_C, 1 hit |
SMARTi | View protein in SMART SM00977, TilS_C, 1 hit |
TIGRFAMsi | TIGR02433, lysidine_TilS_C, 1 hit TIGR02432, lysidine_TilS_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P37563-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSVKDFLNK HNLTLKGATI IVGVSGGPDS MALLHALHTL CGRSANVIAA
60 70 80 90 100
HVDHRFRGAE SEEDMRFVQA YCKAEQLVCE TAQINVTAYA QEKGLNKQAA
110 120 130 140 150
ARDCRYQFFE EIMSKHQADY LALAHHGDDQ VETMLMKLAK GTLGTGLAGM
160 170 180 190 200
QPVRRFGTGR IIRPFLTITK EEILHYCHEN GLSYRTDESN AKDDYTRNRF
210 220 230 240 250
RKTVLPFLKQ ESPDVHKRFQ KVSEALTEDE QFLQSLTKDE MNKVITSQSN
260 270 280 290 300
TSVEINSSQL LALPMPLQRR GVQLILNYLY ENVPSSFSAH HIQQFLDWAE
310 320 330 340 350
NGGPSGVLDF PKGLKVVKSY QTCLFTFEQW QCKNVPFEYQ ISGAADETAV
360 370 380 390 400
LPNGYLIEAR HYADSPEEHG NAVFITSEKK VRFPLTIRTR KAGDRIKLKG
410 420 430 440 450
MNGSKKVKDI FIDKKLPLQE RDNWPIVTDA SGEIIWIPGL KKSIFEDLVI
460 470
PNSDRIVLQY RQHEKCRGQA KS
Sequence cautioni
The sequence BAA05302 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05302.1 Different initiation. AL009126 Genomic DNA Translation: CAB11843.1 |
PIRi | S66097 |
RefSeqi | NP_387948.1, NC_000964.3 WP_003243704.1, NZ_JNCM01000028.1 |
Genome annotation databases
EnsemblBacteriai | CAB11843; CAB11843; BSU_00670 |
GeneIDi | 936922 |
KEGGi | bsu:BSU00670 |
PATRICi | fig|224308.179.peg.67 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05302.1 Different initiation. AL009126 Genomic DNA Translation: CAB11843.1 |
PIRi | S66097 |
RefSeqi | NP_387948.1, NC_000964.3 WP_003243704.1, NZ_JNCM01000028.1 |
3D structure databases
SMRi | P37563 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P37563, 2 interactors |
STRINGi | 224308.BSU00670 |
Proteomic databases
PaxDbi | P37563 |
PRIDEi | P37563 |
Genome annotation databases
EnsemblBacteriai | CAB11843; CAB11843; BSU_00670 |
GeneIDi | 936922 |
KEGGi | bsu:BSU00670 |
PATRICi | fig|224308.179.peg.67 |
Phylogenomic databases
eggNOGi | COG0037, Bacteria |
InParanoidi | P37563 |
OMAi | QQYGMNA |
PhylomeDBi | P37563 |
Enzyme and pathway databases
BioCyci | BSUB:BSU00670-MONOMER |
BRENDAi | 6.3.4.19, 658 |
Family and domain databases
CDDi | cd01992, PP-ATPase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01161, tRNA_Ile_lys_synt, 1 hit |
InterProi | View protein in InterPro IPR012796, Lysidine-tRNA-synth_C IPR014729, Rossmann-like_a/b/a_fold IPR011063, TilS/TtcA_N IPR012094, tRNA_Ile_lys_synt IPR012795, tRNA_Ile_lys_synt_N IPR015262, tRNA_Ile_lys_synt_subst-bd |
PANTHERi | PTHR43033, PTHR43033, 1 hit |
Pfami | View protein in Pfam PF01171, ATP_bind_3, 1 hit PF09179, TilS, 1 hit PF11734, TilS_C, 1 hit |
SMARTi | View protein in SMART SM00977, TilS_C, 1 hit |
TIGRFAMsi | TIGR02433, lysidine_TilS_C, 1 hit TIGR02432, lysidine_TilS_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TILS_BACSU | |
Accessioni | P37563Primary (citable) accession number: P37563 Secondary accession number(s): O31416 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families