UniProtKB - P37528 (PDXT_BACSU)
Protein
Pyridoxal 5'-phosphate synthase subunit PdxT
Gene
pdxT
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation1 Publication
Catalytic activityi
- aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H+ + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphateUniRule annotation1 PublicationEC:4.3.3.6UniRule annotation1 Publication
- EC:3.5.1.2UniRule annotation1 Publication
Kineticsi
kcat is 7.60 min(-1) for glutaminase activity.1 Publication
- KM=0.99 mM for L-glutamine1 Publication
: pyridoxal 5'-phosphate biosynthesis Pathwayi
This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 79 | NucleophileUniRule annotationCurated | 1 | |
Binding sitei | 106 | L-glutamineUniRule annotation1 Publication | 1 | |
Active sitei | 170 | Charge relay systemUniRule annotationCurated | 1 | |
Active sitei | 172 | Charge relay systemUniRule annotationCurated | 1 |
GO - Molecular functioni
- glutaminase activity Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
- pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity Source: UniProtKB-UniRule
GO - Biological processi
- glutamine catabolic process Source: UniProtKB-UniRule
- pyridoxal phosphate biosynthetic process Source: GO_Central
- pyridoxine metabolic process Source: GO_Central
- vitamin B6 biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Lyase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | BSUB:BSU00120-MONOMER MetaCyc:MONOMER-15503 |
BRENDAi | 4.3.3.6 658 |
UniPathwayi | UPA00245 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pdxTUniRule annotation Synonyms:yaaE Ordered Locus Names:BSU00120 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: GO_Central
- glutaminase complex Source: GO_Central
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | Q → A: 3-fold reduction in glutaminase activity. 1 Publication | 1 | |
Mutagenesisi | 10 | Q → E: Almost no activity. 1 Publication | 1 | |
Mutagenesisi | 10 | Q → N: 10-fold reduction in glutaminase activity. 1 Publication | 1 | |
Mutagenesisi | 15 | E → A: Almost no effect on glutaminase activity. 1 Publication | 1 | |
Mutagenesisi | 48 | E → A: No activity, disturbing interaction with PdxS. 1 Publication | 1 | |
Mutagenesisi | 106 | R → A: No activity, disturbing interaction with PdxS. 1 Publication | 1 | |
Mutagenesisi | 135 | R → A: No activity, disturbing interaction with PdxS. 1 Publication | 1 | |
Mutagenesisi | 170 | H → N: No activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135632 | 1 – 196 | Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST | 196 |
Proteomic databases
PaxDbi | P37528 |
PRIDEi | P37528 |
Interactioni
Subunit structurei
In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.
UniRule annotation1 PublicationBinary interactionsi
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-57719N |
IntActi | P37528, 2 interactors |
MINTi | P37528 |
STRINGi | 224308.BSU00120 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37528 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37528 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 47 – 49 | L-glutamine bindingUniRule annotation1 Publication | 3 | |
Regioni | 134 – 135 | L-glutamine bindingUniRule annotation1 Publication | 2 |
Sequence similaritiesi
Belongs to the glutaminase PdxT/SNO family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | ENOG4108UHX Bacteria COG0311 LUCA |
HOGENOMi | HOG000039949 |
InParanoidi | P37528 |
KOi | K08681 |
OMAi | PVYGTCA |
PhylomeDBi | P37528 |
Family and domain databases
CDDi | cd01749 GATase1_PB, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_01615 PdxT, 1 hit |
InterProi | View protein in InterPro IPR029062 Class_I_gatase-like IPR002161 PdxT/SNO IPR021196 PdxT/SNO_CS |
PANTHERi | PTHR31559 PTHR31559, 1 hit |
Pfami | View protein in Pfam PF01174 SNO, 1 hit |
PIRSFi | PIRSF005639 Glut_amidoT_SNO, 1 hit |
SUPFAMi | SSF52317 SSF52317, 1 hit |
TIGRFAMsi | TIGR03800 PLP_synth_Pdx2, 1 hit |
PROSITEi | View protein in PROSITE PS01236 PDXT_SNO_1, 1 hit PS51130 PDXT_SNO_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P37528-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLTIGVLGLQ GAVREHIHAI EACGAAGLVV KRPEQLNEVD GLILPGGEST
60 70 80 90 100
TMRRLIDTYQ FMEPLREFAA QGKPMFGTCA GLIILAKEIA GSDNPHLGLL
110 120 130 140 150
NVVVERNSFG RQVDSFEADL TIKGLDEPFT GVFIRAPHIL EAGENVEVLS
160 170 180 190
EHNGRIVAAK QGQFLGCSFH PELTEDHRVT QLFVEMVEEY KQKALV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05248.1 AL009126 Genomic DNA Translation: CAB11788.1 |
PIRi | S66042 |
RefSeqi | NP_387893.1, NC_000964.3 WP_003226797.1, NZ_JNCM01000024.1 |
Genome annotation databases
EnsemblBacteriai | CAB11788; CAB11788; BSU00120 |
GeneIDi | 939971 |
KEGGi | bsu:BSU00120 |
PATRICi | fig|224308.179.peg.12 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05248.1 AL009126 Genomic DNA Translation: CAB11788.1 |
PIRi | S66042 |
RefSeqi | NP_387893.1, NC_000964.3 WP_003226797.1, NZ_JNCM01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R9G | X-ray | 2.50 | A/B | 1-196 | [»] | |
2NV0 | X-ray | 1.73 | A/B | 1-196 | [»] | |
2NV2 | X-ray | 2.12 | B/D/F/H/J/L/N/P/R/T/V/X | 1-196 | [»] | |
SMRi | P37528 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-57719N |
IntActi | P37528, 2 interactors |
MINTi | P37528 |
STRINGi | 224308.BSU00120 |
Proteomic databases
PaxDbi | P37528 |
PRIDEi | P37528 |
Genome annotation databases
EnsemblBacteriai | CAB11788; CAB11788; BSU00120 |
GeneIDi | 939971 |
KEGGi | bsu:BSU00120 |
PATRICi | fig|224308.179.peg.12 |
Phylogenomic databases
eggNOGi | ENOG4108UHX Bacteria COG0311 LUCA |
HOGENOMi | HOG000039949 |
InParanoidi | P37528 |
KOi | K08681 |
OMAi | PVYGTCA |
PhylomeDBi | P37528 |
Enzyme and pathway databases
UniPathwayi | UPA00245 |
BioCyci | BSUB:BSU00120-MONOMER MetaCyc:MONOMER-15503 |
BRENDAi | 4.3.3.6 658 |
Miscellaneous databases
EvolutionaryTracei | P37528 |
Family and domain databases
CDDi | cd01749 GATase1_PB, 1 hit |
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_01615 PdxT, 1 hit |
InterProi | View protein in InterPro IPR029062 Class_I_gatase-like IPR002161 PdxT/SNO IPR021196 PdxT/SNO_CS |
PANTHERi | PTHR31559 PTHR31559, 1 hit |
Pfami | View protein in Pfam PF01174 SNO, 1 hit |
PIRSFi | PIRSF005639 Glut_amidoT_SNO, 1 hit |
SUPFAMi | SSF52317 SSF52317, 1 hit |
TIGRFAMsi | TIGR03800 PLP_synth_Pdx2, 1 hit |
PROSITEi | View protein in PROSITE PS01236 PDXT_SNO_1, 1 hit PS51130 PDXT_SNO_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDXT_BACSU | |
Accessioni | P37528Primary (citable) accession number: P37528 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | October 16, 2019 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families - Bacillus subtilis
Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references