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UniProtKB - P37487 (PPAC_BACSU)
Protein
Manganese-dependent inorganic pyrophosphatase
Gene
ppaC
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalytic activityi
- EC:3.6.1.1
Cofactori
Mn2+Note: Binds 2 manganese ions per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 9 | Manganese 1 | 1 | |
Metal bindingi | 13 | Manganese 1 | 1 | |
Metal bindingi | 15 | Manganese 2 | 1 | |
Metal bindingi | 75 | Manganese 1 | 1 | |
Metal bindingi | 75 | Manganese 2 | 1 | |
Metal bindingi | 97 | Manganese 2 | 1 | |
Metal bindingi | 149 | Manganese 2 | 1 |
GO - Molecular functioni
- inorganic diphosphatase activity Source: UniProtKB-UniRule
- manganese ion binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU40550-MONOMER |
BRENDAi | 3.6.1.1, 658 |
Names & Taxonomyi
Protein namesi | Recommended name: Manganese-dependent inorganic pyrophosphatase (EC:3.6.1.1)Alternative name(s): Pyrophosphate phospho-hydrolase Short name: PPase |
Gene namesi | Name:ppaC Ordered Locus Names:BSU40550 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000158568 | 1 – 309 | Manganese-dependent inorganic pyrophosphataseAdd BLAST | 309 |
Proteomic databases
jPOSTi | P37487 |
PaxDbi | P37487 |
PRIDEi | P37487 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
IntActi | P37487, 1 interactor |
MINTi | P37487 |
STRINGi | 224308.BSU40550 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37487 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37487 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PPase class C family.Curated
Phylogenomic databases
eggNOGi | COG1227, Bacteria |
InParanoidi | P37487 |
OMAi | STPLECW |
PhylomeDBi | P37487 |
Family and domain databases
Gene3Di | 3.10.310.20, 1 hit |
HAMAPi | MF_00207, PPase_C, 1 hit |
InterProi | View protein in InterPro IPR001667, DDH_dom IPR038763, DHH_sf IPR004097, DHHA2 IPR038222, DHHA2_dom_sf IPR022934, Mn-dep_inorganic_PyrPase |
Pfami | View protein in Pfam PF01368, DHH, 1 hit PF02833, DHHA2, 1 hit |
SMARTi | View protein in SMART SM01131, DHHA2, 1 hit |
SUPFAMi | SSF64182, SSF64182, 1 hit |
i Sequence
Sequence statusi: Complete.
P37487-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKILIFGHQ NPDTDTICSA IAYADLKNKL GFNAEPVRLG QVNGETQYAL
60 70 80 90 100
DYFKQESPRL VETAANEVNG VILVDHNERQ QSIKDIEEVQ VLEVIDHHRI
110 120 130 140 150
ANFETAEPLY YRAEPVGCTA TILNKMYKEN NVKIEKEIAG LMLSAIISDS
160 170 180 190 200
LLFKSPTCTD QDVAAAKELA EIAGVDAEEY GLNMLKAGAD LSKKTVEELI
210 220 230 240 250
SLDAKEFTLG SKKVEIAQVN TVDIEDVKKR QAELEAVISK VVAEKNLDLF
260 270 280 290 300
LLVITDILEN DSLALAIGNE AAKVEKAFNV TLENNTALLK GVVSRKKQVV
PVLTDAMAE
Mass spectrometryi
Molecular mass is 34019 Da. Determined by MALDI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05186.1 AL009126 Genomic DNA Translation: CAB16092.1 |
PIRi | S65980 |
RefSeqi | NP_391935.1, NC_000964.3 WP_003226902.1, NZ_JNCM01000034.1 |
Genome annotation databases
EnsemblBacteriai | CAB16092; CAB16092; BSU_40550 |
GeneIDi | 937817 |
KEGGi | bsu:BSU40550 |
PATRICi | fig|224308.179.peg.4391 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05186.1 AL009126 Genomic DNA Translation: CAB16092.1 |
PIRi | S65980 |
RefSeqi | NP_391935.1, NC_000964.3 WP_003226902.1, NZ_JNCM01000034.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1K23 | X-ray | 3.00 | A/B/C/D | 1-309 | [»] | |
1WPM | X-ray | 2.05 | A/B | 1-309 | [»] | |
1WPN | X-ray | 1.30 | A/B | 1-188 | [»] | |
2HAW | X-ray | 1.75 | A/B | 1-309 | [»] | |
2IW4 | X-ray | 2.15 | A/B | 1-309 | [»] | |
SMRi | P37487 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P37487, 1 interactor |
MINTi | P37487 |
STRINGi | 224308.BSU40550 |
Proteomic databases
jPOSTi | P37487 |
PaxDbi | P37487 |
PRIDEi | P37487 |
Genome annotation databases
EnsemblBacteriai | CAB16092; CAB16092; BSU_40550 |
GeneIDi | 937817 |
KEGGi | bsu:BSU40550 |
PATRICi | fig|224308.179.peg.4391 |
Phylogenomic databases
eggNOGi | COG1227, Bacteria |
InParanoidi | P37487 |
OMAi | STPLECW |
PhylomeDBi | P37487 |
Enzyme and pathway databases
BioCyci | BSUB:BSU40550-MONOMER |
BRENDAi | 3.6.1.1, 658 |
Miscellaneous databases
EvolutionaryTracei | P37487 |
PROi | PR:P37487 |
Family and domain databases
Gene3Di | 3.10.310.20, 1 hit |
HAMAPi | MF_00207, PPase_C, 1 hit |
InterProi | View protein in InterPro IPR001667, DDH_dom IPR038763, DHH_sf IPR004097, DHHA2 IPR038222, DHHA2_dom_sf IPR022934, Mn-dep_inorganic_PyrPase |
Pfami | View protein in Pfam PF01368, DHH, 1 hit PF02833, DHHA2, 1 hit |
SMARTi | View protein in SMART SM01131, DHHA2, 1 hit |
SUPFAMi | SSF64182, SSF64182, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPAC_BACSU | |
Accessioni | P37487Primary (citable) accession number: P37487 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families