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UniProtKB - P37477 (SYK_BACSU)
Protein
Lysine--tRNA ligase
Gene
lysS
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalytic activityi
- EC:6.1.1.6
Cofactori
Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 410 | Magnesium 1By similarity | 1 | |
Metal bindingi | 417 | Magnesium 1By similarity | 1 | |
Metal bindingi | 417 | Magnesium 2By similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- lysine-tRNA ligase activity Source: GO_Central
- magnesium ion binding Source: UniProtKB-UniRule
- tRNA binding Source: GO_Central
GO - Biological processi
- lysyl-tRNA aminoacylation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU00820-MONOMER |
SABIO-RKi | P37477 |
Names & Taxonomyi
Protein namesi | Recommended name: Lysine--tRNA ligase (EC:6.1.1.6)Alternative name(s): Lysyl-tRNA synthetase Short name: LysRS |
Gene namesi | Name:lysS Ordered Locus Names:BSU00820 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000152600 | 1 – 499 | Lysine--tRNA ligaseAdd BLAST | 499 |
Proteomic databases
jPOSTi | P37477 |
PaxDbi | P37477 |
PRIDEi | P37477 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
IntActi | P37477, 1 interactor |
MINTi | P37477 |
STRINGi | 224308.BSU00820 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | COG1190, Bacteria |
InParanoidi | P37477 |
OMAi | EIFGEKC |
PhylomeDBi | P37477 |
Family and domain databases
CDDi | cd00775, LysRS_core, 1 hit cd04322, LysRS_N, 1 hit |
Gene3Di | 2.40.50.140, 1 hit 3.30.930.10, 1 hit |
HAMAPi | MF_00252, Lys_tRNA_synth_class2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR002313, Lys-tRNA-ligase_II IPR034762, Lys-tRNA-ligase_II_bac/euk IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PIRSFi | PIRSF039101, LysRS2, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P37477-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQEEHNHEE LNDQLQVRRD KMNQLRDNGI DPFGARFERT HQSQEVISAY
60 70 80 90 100
QDLTKEELEE KAIEVTIAGR MMTKRGKGKA GFAHLQDLEG QIQIYVRKDS
110 120 130 140 150
VGDDQYEIFK SSDLGDLIGV TGKVFKTNVG ELSVKATSFE LLTKALRPLP
160 170 180 190 200
DKYHGLKDVE QRYRQRYLDL IVNPDSKHTF ITRSKIIQAM RRYLDDHGYL
210 220 230 240 250
EVETPTMHSI PGGASARPFI THHNALDIPL YMRIAIELHL KRLIVGGLEK
260 270 280 290 300
VYEIGRVFRN EGVSTRHNPE FTMIELYEAY ADYKDIMSLT ENLVAHIAQE
310 320 330 340 350
VLGTTTIQYG EEQIDLKPEW KRIHMVDAVK EATGVDFWEE VTVEQAREYA
360 370 380 390 400
KEHEVEIKDS MTVGHIINEF FEQKIEETLI QPTFIYGHPV EISPLAKKNP
410 420 430 440 450
EDPRFTDRFE LFIVGREHAN AFTELNDPID QRERFEAQLK EREAGNDEAH
460 470 480 490
LMDEDFVEAL EYGMPPTGGL GIGIDRLVML LTNAPSIRDV LLFPQMRQR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05316.1 AL009126 Genomic DNA Translation: CAB11858.1 |
PIRi | S66111 |
RefSeqi | NP_387963.1, NC_000964.3 WP_003242743.1, NZ_JNCM01000028.1 |
Genome annotation databases
EnsemblBacteriai | CAB11858; CAB11858; BSU_00820 |
GeneIDi | 936911 |
KEGGi | bsu:BSU00820 |
PATRICi | fig|224308.179.peg.82 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05316.1 AL009126 Genomic DNA Translation: CAB11858.1 |
PIRi | S66111 |
RefSeqi | NP_387963.1, NC_000964.3 WP_003242743.1, NZ_JNCM01000028.1 |
3D structure databases
AlphaFoldDBi | P37477 |
SMRi | P37477 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P37477, 1 interactor |
MINTi | P37477 |
STRINGi | 224308.BSU00820 |
Proteomic databases
jPOSTi | P37477 |
PaxDbi | P37477 |
PRIDEi | P37477 |
Genome annotation databases
EnsemblBacteriai | CAB11858; CAB11858; BSU_00820 |
GeneIDi | 936911 |
KEGGi | bsu:BSU00820 |
PATRICi | fig|224308.179.peg.82 |
Phylogenomic databases
eggNOGi | COG1190, Bacteria |
InParanoidi | P37477 |
OMAi | EIFGEKC |
PhylomeDBi | P37477 |
Enzyme and pathway databases
BioCyci | BSUB:BSU00820-MONOMER |
SABIO-RKi | P37477 |
Family and domain databases
CDDi | cd00775, LysRS_core, 1 hit cd04322, LysRS_N, 1 hit |
Gene3Di | 2.40.50.140, 1 hit 3.30.930.10, 1 hit |
HAMAPi | MF_00252, Lys_tRNA_synth_class2, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR002313, Lys-tRNA-ligase_II IPR034762, Lys-tRNA-ligase_II_bac/euk IPR044136, Lys-tRNA-ligase_II_N IPR018149, Lys-tRNA-synth_II_C IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PIRSFi | PIRSF039101, LysRS2, 1 hit |
PRINTSi | PR00982, TRNASYNTHLYS |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00499, lysS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYK_BACSU | |
Accessioni | P37477Primary (citable) accession number: P37477 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families