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UniProtKB - P37474 (MFD_BACSU)
Protein
Transcription-repair-coupling factor
Gene
mfd
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.
UniRule annotationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 651 – 658 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- damaged DNA binding Source: InterPro
- DNA binding Source: GO_Central
- DNA translocase activity Source: GO_Central
- helicase activity Source: UniProtKB-KW
- hydrolase activity Source: UniProtKB-KW
- RNA polymerase core enzyme binding Source: GO_Central
GO - Biological processi
- regulation of transcription, DNA-templated Source: GO_Central
- transcription-coupled nucleotide-excision repair, DNA damage recognition Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU00550-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription-repair-coupling factorUniRule annotation (EC:3.6.4.-UniRule annotation)Short name: TRCFUniRule annotation |
Gene namesi | Name:mfdUniRule annotation Ordered Locus Names:BSU00550 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102162 | 1 – 1177 | Transcription-repair-coupling factorAdd BLAST | 1177 |
Proteomic databases
PaxDbi | P37474 |
PRIDEi | P37474 |
Interactioni
GO - Molecular functioni
- RNA polymerase core enzyme binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 224308.BSU00550 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 638 – 799 | Helicase ATP-bindingUniRule annotationAdd BLAST | 162 | |
Domaini | 820 – 974 | Helicase C-terminalUniRule annotationAdd BLAST | 155 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 752 – 755 | DEEQ box | 4 |
Sequence similaritiesi
In the N-terminal section; belongs to the UvrB family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1197, Bacteria |
InParanoidi | P37474 |
OMAi | WAPPCRE |
PhylomeDBi | P37474 |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits 3.90.1150.50, 1 hit |
HAMAPi | MF_00969, TRCF, 1 hit |
InterProi | View protein in InterPro IPR036101, CarD-like/TRCF_dom_sf IPR003711, CarD-like/TRCF_domain IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR004576, Mfd IPR027417, P-loop_NTPase IPR037235, TRCF-like_C_D7 IPR005118, TRCF_C IPR041471, UvrB_inter |
Pfami | View protein in Pfam PF02559, CarD_CdnL_TRCF, 1 hit PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF03461, TRCF, 1 hit PF17757, UvrB_inter, 1 hit |
SMARTi | View protein in SMART SM01058, CarD_TRCF, 1 hit SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00982, TRCF, 1 hit |
SUPFAMi | SSF141259, SSF141259, 1 hit SSF143517, SSF143517, 1 hit SSF52540, SSF52540, 4 hits |
TIGRFAMsi | TIGR00580, mfd, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P37474-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNIQTFIKE SDDFKSIING LHEGLKEQLL AGLSGSARSV FTSALANETN
60 70 80 90 100
KPIFLITHNL YQAQKVTDDL TSLLEDRSVL LYPVNELISS EIAVASPELR
110 120 130 140 150
AQRLDVINRL TNGEAPIVVA PVAAIRRMLP PVEVWKSSQM LIQVGHDIEP
160 170 180 190 200
DQLASRLVEV GYERSDMVSA PGEFSIRGGI IDIYPLTSEN PVRIELFDTE
210 220 230 240 250
VDSIRSFNSD DQRSIETLTS INIGPAKELI IRPEEKARAM EKIDSGLAAS
260 270 280 290 300
LKKLKADKQK EILHANISHD KERLSEGQTD QELVKYLSYF YEKPASLLDY
310 320 330 340 350
TPDNTLLILD EVSRIHEMEE QLQKEEAEFI TNLLEEGKIL HDIRLSFSFQ
360 370 380 390 400
KIVAEQKRPL LYYSLFLRHV HHTSPQNIVN VSGRQMQSFH GQMNVLAGEM
410 420 430 440 450
ERFKKSNFTV VFLGANKERT QKLSSVLADY DIEAAMTDSK KALVQGQVYI
460 470 480 490 500
MEGELQSGFE LPLMKLAVIT EEELFKNRVK KKPRKQKLTN AERIKSYSEL
510 520 530 540 550
QIGDYVVHIN HGIGKYLGIE TLEINGIHKD YLNIHYQGSD KLYVPVEQID
560 570 580 590 600
QVQKYVGSEG KEPKLYKLGG SEWKRVKKKV ETSVQDIADD LIKLYAEREA
610 620 630 640 650
SKGYAFSPDH EMQREFESAF PYQETEDQLR SIHEIKKDME RERPMDRLLC
660 670 680 690 700
GDVGYGKTEV AIRAAFKAIG DGKQVALLVP TTILAQQHYE TIKERFQDYP
710 720 730 740 750
INIGLLSRFR TRKEANETIK GLKNGTVDIV IGTHRLLSKD VVYKDLGLLI
760 770 780 790 800
IDEEQRFGVT HKEKIKQIKA NVDVLTLTAT PIPRTLHMSM LGVRDLSVIE
810 820 830 840 850
TPPENRFPVQ TYVVEYNGAL VREAIERELA RGGQVYFLYN RVEDIERKAD
860 870 880 890 900
EISMLVPDAK VAYAHGKMTE NELETVMLSF LEGESDVLVS TTIIETGVDI
910 920 930 940 950
PNVNTLIVFD ADKMGLSQLY QLRGRVGRSN RVAYAYFTYR RDKVLTEVAE
960 970 980 990 1000
KRLQAIKEFT ELGSGFKIAM RDLTIRGAGN LLGAQQHGFI DSVGFDLYSQ
1010 1020 1030 1040 1050
MLKEAIEERK GDTAKTEQFE TEIDVELDAY IPETYIQDGK QKIDMYKRFR
1060 1070 1080 1090 1100
SVATIEEKNE LQDEMIDRFG NYPKEVEYLF TVAEMKVYAR QERVELIKQD
1110 1120 1130 1140 1150
KDAVRLTISE EASAEIDGQK LFELGNQYGR QIGLGMEGKK LKISIQTKGR
1160 1170
SADEWLDTVL GMLKGLKDVK KQTISST
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05290.1 AL009126 Genomic DNA Translation: CAB11831.1 |
PIRi | S66085 |
RefSeqi | NP_387936.1, NC_000964.3 WP_003243453.1, NZ_JNCM01000028.1 |
Genome annotation databases
EnsemblBacteriai | CAB11831; CAB11831; BSU_00550 |
GeneIDi | 936653 |
KEGGi | bsu:BSU00550 |
PATRICi | fig|224308.179.peg.55 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05290.1 AL009126 Genomic DNA Translation: CAB11831.1 |
PIRi | S66085 |
RefSeqi | NP_387936.1, NC_000964.3 WP_003243453.1, NZ_JNCM01000028.1 |
3D structure databases
AlphaFoldDBi | P37474 |
SMRi | P37474 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU00550 |
Proteomic databases
PaxDbi | P37474 |
PRIDEi | P37474 |
Protocols and materials databases
DNASUi | 936653 |
Genome annotation databases
EnsemblBacteriai | CAB11831; CAB11831; BSU_00550 |
GeneIDi | 936653 |
KEGGi | bsu:BSU00550 |
PATRICi | fig|224308.179.peg.55 |
Phylogenomic databases
eggNOGi | COG1197, Bacteria |
InParanoidi | P37474 |
OMAi | WAPPCRE |
PhylomeDBi | P37474 |
Enzyme and pathway databases
BioCyci | BSUB:BSU00550-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits 3.90.1150.50, 1 hit |
HAMAPi | MF_00969, TRCF, 1 hit |
InterProi | View protein in InterPro IPR036101, CarD-like/TRCF_dom_sf IPR003711, CarD-like/TRCF_domain IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR004576, Mfd IPR027417, P-loop_NTPase IPR037235, TRCF-like_C_D7 IPR005118, TRCF_C IPR041471, UvrB_inter |
Pfami | View protein in Pfam PF02559, CarD_CdnL_TRCF, 1 hit PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit PF03461, TRCF, 1 hit PF17757, UvrB_inter, 1 hit |
SMARTi | View protein in SMART SM01058, CarD_TRCF, 1 hit SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00982, TRCF, 1 hit |
SUPFAMi | SSF141259, SSF141259, 1 hit SSF143517, SSF143517, 1 hit SSF52540, SSF52540, 4 hits |
TIGRFAMsi | TIGR00580, mfd, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MFD_BACSU | |
Accessioni | P37474Primary (citable) accession number: P37474 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families