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UniProtKB - P37464 (SYS_BACSU)
Protein
Serine--tRNA ligase
Gene
serS
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
UniRule annotationCatalytic activityi
: selenocysteinyl-tRNA(Sec) biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).UniRule annotation This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 285 | SerineUniRule annotation | 1 | |
Binding sitei | 385 | SerineUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 262 – 264 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 349 – 352 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- serine-tRNA ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- selenocysteine biosynthetic process Source: UniProtKB-UniRule
- selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
- seryl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU00130-MONOMER |
UniPathwayi | UPA00906;UER00895 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine--tRNA ligaseUniRule annotation (EC:6.1.1.11UniRule annotation)Alternative name(s): Seryl-tRNA synthetaseUniRule annotation Short name: SerRSUniRule annotation Seryl-tRNA(Ser/Sec) synthetaseUniRule annotation |
Gene namesi | Name:serSUniRule annotation Ordered Locus Names:BSU00130 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122005 | 1 – 425 | Serine--tRNA ligaseAdd BLAST | 425 |
Proteomic databases
jPOSTi | P37464 |
PaxDbi | P37464 |
PRIDEi | P37464 |
Interactioni
Subunit structurei
Homodimer. The tRNA molecule binds across the dimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 224308.BSU00130 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 231 – 233 | Serine bindingUniRule annotation | 3 |
Domaini
Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.UniRule annotation
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0172, Bacteria |
InParanoidi | P37464 |
OMAi | SPCFRRE |
PhylomeDBi | P37464 |
Family and domain databases
CDDi | cd00770, SerRS_core, 1 hit |
Gene3Di | 1.10.287.40, 1 hit 3.30.930.10, 1 hit |
HAMAPi | MF_00176, Ser_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR002317, Ser-tRNA-ligase_type_1 IPR015866, Ser-tRNA-synth_1_N IPR042103, SerRS_1_N_sf IPR033729, SerRS_core IPR010978, tRNA-bd_arm |
PANTHERi | PTHR43697, PTHR43697, 1 hit |
Pfami | View protein in Pfam PF02403, Seryl_tRNA_N, 1 hit PF00587, tRNA-synt_2b, 1 hit |
PIRSFi | PIRSF001529, Ser-tRNA-synth_IIa, 1 hit |
PRINTSi | PR00981, TRNASYNTHSER |
SUPFAMi | SSF46589, SSF46589, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00414, serS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
P37464-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLDTKMLRAN FQEIKAKLVH KGEDLTDFDK FEALDDRRRE LIGKVEELKG
60 70 80 90 100
KRNEVSQQVA VLKREKKDAD HIIKEMREVG EEIKKLDEEL RTVEAELDTI
110 120 130 140 150
LLSIPNIPHE SVPVGETEDD NVEVRKWGEK PSFAYEPKPH WDIADELGIL
160 170 180 190 200
DFERAAKVTG SRFVFYKGLG ARLERALYNF MLDLHVDEYN YTEVIPPYMV
210 220 230 240 250
NRASMTGTGQ LPKFEEDAFK IREEDYFLIP TAEVPITNMH RDEILSGDSL
260 270 280 290 300
PINYAAFSAC FRSEAGSAGR DTRGLIRQHQ FNKVELVKFV KPEDSYEELE
310 320 330 340 350
KLTNQAERVL QLLELPYRVM SMCTGDLGFT AAKKYDIEVW IPSQDTYREI
360 370 380 390 400
SSCSNFEAFQ ARRANIRFRR EAKGKPEHVH TLNGSGLAVG RTVAAILENY
410 420
QQEDGSVVIP KVLRPYMGNR EVMKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05249.1 AL009126 Genomic DNA Translation: CAB11789.1 |
PIRi | S66043 |
RefSeqi | NP_387894.1, NC_000964.3 WP_003247131.1, NZ_JNCM01000024.1 |
Genome annotation databases
EnsemblBacteriai | CAB11789; CAB11789; BSU_00130 |
GeneIDi | 939994 |
KEGGi | bsu:BSU00130 |
PATRICi | fig|224308.179.peg.13 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D26185 Genomic DNA Translation: BAA05249.1 AL009126 Genomic DNA Translation: CAB11789.1 |
PIRi | S66043 |
RefSeqi | NP_387894.1, NC_000964.3 WP_003247131.1, NZ_JNCM01000024.1 |
3D structure databases
SMRi | P37464 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU00130 |
Proteomic databases
jPOSTi | P37464 |
PaxDbi | P37464 |
PRIDEi | P37464 |
Genome annotation databases
EnsemblBacteriai | CAB11789; CAB11789; BSU_00130 |
GeneIDi | 939994 |
KEGGi | bsu:BSU00130 |
PATRICi | fig|224308.179.peg.13 |
Phylogenomic databases
eggNOGi | COG0172, Bacteria |
InParanoidi | P37464 |
OMAi | SPCFRRE |
PhylomeDBi | P37464 |
Enzyme and pathway databases
UniPathwayi | UPA00906;UER00895 |
BioCyci | BSUB:BSU00130-MONOMER |
Family and domain databases
CDDi | cd00770, SerRS_core, 1 hit |
Gene3Di | 1.10.287.40, 1 hit 3.30.930.10, 1 hit |
HAMAPi | MF_00176, Ser_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR002317, Ser-tRNA-ligase_type_1 IPR015866, Ser-tRNA-synth_1_N IPR042103, SerRS_1_N_sf IPR033729, SerRS_core IPR010978, tRNA-bd_arm |
PANTHERi | PTHR43697, PTHR43697, 1 hit |
Pfami | View protein in Pfam PF02403, Seryl_tRNA_N, 1 hit PF00587, tRNA-synt_2b, 1 hit |
PIRSFi | PIRSF001529, Ser-tRNA-synth_IIa, 1 hit |
PRINTSi | PR00981, TRNASYNTHSER |
SUPFAMi | SSF46589, SSF46589, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00414, serS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYS_BACSU | |
Accessioni | P37464Primary (citable) accession number: P37464 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families