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Protein

Bifunctional ligase/repressor BirA

Gene

birA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.UniRule annotation

Catalytic activityi

ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112BiotinUniRule annotation1
Binding sitei183BiotinUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi22 – 41H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Ligase, Repressor
Biological processTranscription, Transcription regulation
LigandATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-4178-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ligase/repressor BirAUniRule annotation
Alternative name(s):
Biotin operon repressorUniRule annotation
Biotin--[acetyl-CoA-carboxylase] ligaseUniRule annotation (EC:6.3.4.15UniRule annotation)
Biotin--protein ligaseUniRule annotation
Biotin-[acetyl-CoA carboxylase] synthetaseUniRule annotation
Gene namesi
Name:birAUniRule annotation
Ordered Locus Names:STM4138
ORF Names:STMF1.2
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649351 – 320Bifunctional ligase/repressor BirAAdd BLAST320

Proteomic databases

PaxDbiP37416
PRIDEiP37416

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4138

Structurei

3D structure databases

ProteinModelPortaliP37416
SMRiP37416
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 254BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni89 – 91Biotin bindingUniRule annotation3
Regioni116 – 118Biotin bindingUniRule annotation3

Sequence similaritiesi

Belongs to the biotin--protein ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105HJX Bacteria
COG0340 LUCA
COG1654 LUCA
HOGENOMiHOG000041812
KOiK03524
OMAiRAAVWKH
PhylomeDBiP37416

Family and domain databases

CDDicd16442 BPL, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00978 Bifunct_BirA, 1 hit
InterProiView protein in InterPro
IPR030855 Bifunct_BirA
IPR004408 Biotin_CoA_COase_ligase
IPR004409 Biotin_operon_repress_HTH
IPR003142 BPL_C
IPR004143 BPL_LPL_catalytic
IPR013196 HTH_11
IPR008988 Transcriptional_repressor_C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12835:SF5 PTHR12835:SF5, 1 hit
PfamiView protein in Pfam
PF02237 BPL_C, 1 hit
PF03099 BPL_LplA_LipB, 1 hit
PF08279 HTH_11, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50037 SSF50037, 1 hit
TIGRFAMsiTIGR00121 birA_ligase, 1 hit
TIGR00122 birA_repr_reg, 1 hit
PROSITEiView protein in PROSITE
PS51733 BPL_LPL_CATALYTIC, 1 hit

Sequencei

Sequence statusi: Complete.

P37416-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDTTVPLTL ISLLADGEFH SGEQLGERLG MSRAAINKHI QTLRDWGVDV
60 70 80 90 100
FTVPGKGYSL PEPIQLLDAD RIHSQLDSGN VAVLPVIDST NQYLLDRIGE
110 120 130 140 150
LRSGDACVAE YQQAGRGRRG RKWFSPFGAN LYLSMYWRLE QGPAAAIGLS
160 170 180 190 200
LVIGIVMAEV LRKLGADKVR VKWPNDLYLL DRKLAGILVE LTGKTGDAAQ
210 220 230 240 250
IVIGAGINMA MRRVEEDVIN QGWITLQEAG ITLDRNMLAA KLIYKLRAAL
260 270 280 290 300
ELFEQEGLSP YLSRWKKLDN FIDRPVKLII GDKEIFGISR GIDTQGALLL
310 320
EQDGVIKPWM GGEISLRSAE
Length:320
Mass (Da):35,395
Last modified:August 29, 2001 - v2
Checksum:i010E8483093297F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100 – 101EL → DV (PubMed:8048842).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170176 Genomic DNA Translation: AAF33493.1
AE006468 Genomic DNA Translation: AAL22971.1
L14816 Genomic DNA No translation available.
RefSeqiNP_463012.1, NC_003197.2
WP_000655752.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL22971; AAL22971; STM4138
GeneIDi1255664
KEGGistm:STM4138
PATRICifig|99287.12.peg.4354

Similar proteinsi

Entry informationi

Entry nameiBIRA_SALTY
AccessioniPrimary (citable) accession number: P37416
Secondary accession number(s): Q9L9K4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 29, 2001
Last modified: April 25, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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