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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63PotassiumBy similarity1
Metal bindingi119PotassiumBy similarity1
Binding sitei152NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi155PotassiumBy similarity1
Binding sitei298NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei412NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi382 – 386ADPBy similarity5
Nucleotide bindingi402 – 411ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-418-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:ML0373
ORF Names:B229_C2_201, u229g
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0373

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001190471 – 473Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST473

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0373

Structurei

3D structure databases

ProteinModelPortaliP37391
SMRiP37391
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 205YjeF N-terminalAdd BLAST196
Domaini210 – 473YjeF C-terminalAdd BLAST264

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 203NAD(P)H-hydrate epimeraseBy similarityAdd BLAST203
Regioni62 – 66NAD(P)HX (for epimerase activity)By similarity5
Regioni123 – 129NAD(P)HX (for epimerase activity)By similarity7
Regioni210 – 473ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST264
Regioni348 – 354NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiENOG4107QTJ Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228405
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01049 YJEF_C_1, 1 hit
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

P37391-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRHYYSVAAI RDAEASLLAS LPDGVLMKRA AYGLASVIIR ELAVRTGGVT
60 70 80 90 100
GRRVCAVVGS GDNGGDALWA ATFLRRRGAA ADAVLLNPDR VHRKALVAFR
110 120 130 140 150
KAGGRIVENV SAATDLVIDG VVGISGSGPL RPAAAAVFAT VSASGVPVVA
160 170 180 190 200
VDLPSGIDVV TGVINGPAVH AALTVTFGGL KPVHALADCG DVTLVDIGLD
210 220 230 240 250
LPDSDILGLQ AADVAAYWPV PGVHDDKYTQ GVTGVLAGSS TYPGAAVLCT
260 270 280 290 300
GAAVAATSGM VRYAGSAYTQ VLAHWPEVIA SATPTAAGRV QSWVVGPGLG
310 320 330 340 350
IDATATAALW FALETDLPVL VDADGLTMLA AHPDLVINRN APTVLTPHAS
360 370 380 390 400
EFARLAGTPP GDDRVGACRK LADSFGATVL LKGNVTVIAD PGGPVYLNPA
410 420 430 440 450
GQSWAATAGS GDVLSGMIGA LLAAGLPAAE AAAAAAFVHA RAAALSAADP
460 470
GPGDVPTSAS RMVSHIRTAL AAL
Length:473
Mass (Da):47,227
Last modified:April 27, 2001 - v2
Checksum:i29569CBBD666C81C
GO

Sequence cautioni

The sequence AAA17298 differs from that shown. Reason: Frameshift at positions 76 and 429.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66 – 67DA → ES (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00020 Genomic DNA Translation: AAA17298.1 Frameshift.
AL583918 Genomic DNA Translation: CAC29881.1
PIRiE86955
S72984
RefSeqiNP_301366.1, NC_002677.1
WP_010907690.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC29881; CAC29881; CAC29881
GeneIDi909029
KEGGimle:ML0373
PATRICifig|272631.5.peg.630

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00020 Genomic DNA Translation: AAA17298.1 Frameshift.
AL583918 Genomic DNA Translation: CAC29881.1
PIRiE86955
S72984
RefSeqiNP_301366.1, NC_002677.1
WP_010907690.1, NC_002677.1

3D structure databases

ProteinModelPortaliP37391
SMRiP37391
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0373

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC29881; CAC29881; CAC29881
GeneIDi909029
KEGGimle:ML0373
PATRICifig|272631.5.peg.630

Organism-specific databases

LepromaiML0373

Phylogenomic databases

eggNOGiENOG4107QTJ Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228405
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-418-MONOMER

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01049 YJEF_C_1, 1 hit
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNR_MYCLE
AccessioniPrimary (citable) accession number: P37391
Secondary accession number(s): Q9CCV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 27, 2001
Last modified: October 10, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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