Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene

menH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro.2 Publications

Catalytic activityi

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate.UniRule annotation1 Publication

Kineticsi

  1. KM=10.1 µM for SEPHCHC (at 25 degrees Celsius and pH 7)1 Publication

    Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

    This protein is involved in step 3 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
    Proteins known to be involved in the 7 steps of the subpathway in this organism are:
    1. Isochorismate synthase MenF (menF)
    2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
    3. 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
    4. o-succinylbenzoate synthase (menC)
    5. 2-succinylbenzoate--CoA ligase (menE)
    6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
    7. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (menI)
    This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

    Pathwayi: menaquinone biosynthesis

    This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

    GO - Molecular functioni

    GO - Biological processi

    • menaquinone biosynthetic process Source: EcoCyc

    Keywordsi

    Molecular functionLyase
    Biological processMenaquinone biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12438-MONOMER
    MetaCyc:EG12438-MONOMER
    BRENDAi3.1.2.2 2026
    UniPathwayiUPA00079
    UPA01057; UER00900

    Protein family/group databases

    ESTHERiecoli-YFBB MenH_SHCHC
    MEROPSiS33.996

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseUniRule annotation (EC:4.2.99.20UniRule annotation)
    Short name:
    SHCHC synthaseUniRule annotation
    Gene namesi
    Name:menHUniRule annotation
    Synonyms:yfbB
    Ordered Locus Names:b2263, JW2258
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12438 menH

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi86S → A: 1400-fold decrease in catalytic activity. 1 Publication1
    Mutagenesisi210D → A: Loss of activity. 1 Publication1
    Mutagenesisi232H → A: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001691741 – 2522-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseAdd BLAST252

    Proteomic databases

    PaxDbiP37355
    PRIDEiP37355

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation1 Publication

    Protein-protein interaction databases

    BioGridi4260505, 14 interactors
    IntActiP37355, 5 interactors
    STRINGi316385.ECDH10B_2424

    Structurei

    Secondary structure

    1252
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 8Combined sources4
    Beta strandi16 – 20Combined sources5
    Turni27 – 30Combined sources4
    Helixi31 – 34Combined sources4
    Helixi35 – 37Combined sources3
    Beta strandi40 – 45Combined sources6
    Helixi51 – 53Combined sources3
    Helixi61 – 74Combined sources14
    Beta strandi79 – 85Combined sources7
    Helixi87 – 98Combined sources12
    Beta strandi103 – 110Combined sources8
    Helixi118 – 137Combined sources20
    Helixi140 – 147Combined sources8
    Helixi151 – 153Combined sources3
    Helixi158 – 168Combined sources11
    Helixi173 – 182Combined sources10
    Helixi185 – 187Combined sources3
    Helixi192 – 196Combined sources5
    Beta strandi199 – 207Combined sources9
    Helixi211 – 218Combined sources8
    Beta strandi220 – 227Combined sources8
    Helixi234 – 237Combined sources4
    Helixi239 – 250Combined sources12

    3D structure databases

    ProteinModelPortaliP37355
    SMRiP37355
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. MenH family.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4107J5P Bacteria
    COG0596 LUCA
    HOGENOMiHOG000028072
    InParanoidiP37355
    KOiK08680
    OMAiLNDWYQQ
    PhylomeDBiP37355

    Family and domain databases

    Gene3Di3.40.50.1820, 1 hit
    HAMAPiMF_01660 MenH, 1 hit
    InterProiView protein in InterPro
    IPR029058 AB_hydrolase
    IPR000073 AB_hydrolase_1
    IPR022485 SHCHC_synthase_MenH
    PfamiView protein in Pfam
    PF12697 Abhydrolase_6, 1 hit
    SUPFAMiSSF53474 SSF53474, 1 hit
    TIGRFAMsiTIGR03695 menH_SHCHC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P37355-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MILHAQAKHG KPGLPWLVFL HGFSGDCHEW QEVGEAFADY SRLYVDLPGH
    60 70 80 90 100
    GGSAAISVDG FDDVTDLLRK TLVSYNILDF WLVGYSLGGR VAMMAACQGL
    110 120 130 140 150
    AGLCGVIVEG GHPGLQNAEQ RAERQRSDRQ WVQRFLTEPL TAVFADWYQQ
    160 170 180 190 200
    PVFASLNDDQ RRELVALRSN NNGATLAAML EATSLAVQPD LRANLSARTF
    210 220 230 240 250
    AFYYLCGERD SKFRALAAEL AADCHVIPRA GHNAHRENPA GVIASLAQIL

    RF
    Length:252
    Mass (Da):27,682
    Last modified:April 27, 2001 - v2
    Checksum:iFE7D77861842B68C
    GO

    Sequence cautioni

    The sequence AAA24151 differs from that shown. Reason: Frameshift at position 182.Curated
    The sequence M93421 differs from that shown. Reason: Frameshift at position 182.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti176L → V in AAA24151 (Ref. 1) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L35030 Genomic DNA Translation: AAA24151.1 Frameshift.
    U00096 Genomic DNA Translation: AAC75323.1
    AP009048 Genomic DNA Translation: BAA16087.2
    M93421 Genomic DNA No translation available.
    PIRiE64997
    RefSeqiNP_416766.1, NC_000913.3
    WP_000600499.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75323; AAC75323; b2263
    BAA16087; BAA16087; BAA16087
    GeneIDi946736
    KEGGiecj:JW2258
    eco:b2263
    PATRICifig|1411691.4.peg.4473

    Similar proteinsi

    Entry informationi

    Entry nameiMENH_ECOLI
    AccessioniPrimary (citable) accession number: P37355
    Secondary accession number(s): P76477, P76932
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: April 27, 2001
    Last modified: March 28, 2018
    This is version 137 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health