dhaM

Functionⁱ

Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.1 Publication

Miscellaneous

Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKLM has no transport activity.1 Publication

Caution

Was reported to be a protein deacetylase that removes acetyl groups on specific lysine residues in target proteins (PubMed:26716769). However, later experiments demonstrate that this protein does not have any protein deacetylase activity; the discrepancy observed seems to be due to contaminants having proteolytic activity (PubMed:29939131).2 Publications

Catalytic activityⁱ

Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate.1 Publication

Kineticsⁱ

kcat is 2.8 sec(-1). Values measured with the DhaKLM complex.1 Publication

K_M=
45 µM for dihydroxyacetone
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "The dihydroxyacetone kinase of Escherichia coli utilizes a phosphoprotein instead of ATP as phosphoryl donor."
  Gutknecht R., Beutler R., Garcia-Alles L.F., Baumann U., Erni B.
  EMBO J. 20:2480-2486(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION AS A PHOSPHORYL DONOR FOR DIHYDROXYACETONE KINASE, CATALYTIC ACTIVITY OF THE COMPLEX, BIOPHYSICOCHEMICAL PROPERTIES, PHOSPHORYLATION, SUBUNIT, MUTAGENESIS OF HIS-9; HIS-169 AND HIS-430, ACTIVE SITE, DOMAIN.

Pathwayⁱ: glycerol degradation

This protein is involved in the pathway glycerol degradation, which is part of Polyol metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway glycerol degradation and in Polyol metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	9	Tele-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication	1
Active siteⁱ	169	Pros-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication	1
Active siteⁱ	430	Tele-phosphohistidine intermediate1 Publication	1

GO - Molecular functionⁱ

phosphoenolpyruvate-glycerone phosphotransferase activity Source: UniProtKB
protein deacetylase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 26716769

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to DNA damage stimulus
Source: EcoliWiki
Inferred from expression patternⁱ
- 11967071
peptidyl-lysine deacetylation
Source: EcoCyc
Inferred from direct assayⁱ
- 26716769
phosphoenolpyruvate-dependent sugar phosphotransferase system Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase

Molecular function

Transferase

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12399-MONOMER MetaCyc:EG12399-MONOMER
BRENDAⁱ	2.7.1.29 2026
UniPathwayⁱ	UPA00616

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM1 Publication (EC:2.7.1.1211 Publication) Alternative name(s): Dihydroxyacetone kinase subunit M1 Publication
Gene namesⁱ	Name:dhaM1 Publication Synonyms:ycgC Ordered Locus Names:b1198, JW5185
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12399 dhaM

EcoGeneⁱ

EG12399 dhaM

Subcellular locationⁱ

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323
integral component of membrane Source: InterPro

View the complete GO annotation on QuickGO ...

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	9	H → A: Loss of phosphotransfer activity. 1 Publication	1
Mutagenesisⁱ	169	H → A: Loss of phosphotransfer activity. 1 Publication	1
Mutagenesisⁱ	430	H → A: Loss of phosphotransfer activity. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000186713	1 – 472	PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaMAdd BLAST		472

Proteomic databases

PaxDbⁱ	P37349
PRIDEⁱ	P37349

2D gel databases

SWISS-2DPAGEⁱ	P37349

SWISS-2DPAGEⁱ

P37349

Interactionⁱ

Subunit structureⁱ

Homodimer (PubMed:11350937). The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.1 Publication

Protein-protein interaction databases

BioGridⁱ	4260761, 22 interactors
ComplexPortalⁱ	CPX-2634 Dha Kinase
Database of interacting proteins More...DIPⁱ	DIP-11551N
IntActⁱ	P37349, 7 interactors
STRINGⁱ	316385.ECDH10B_1251

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P37349
SMRⁱ	P37349
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 135	PTS EIIA type-4PROSITE-ProRule annotation1 PublicationAdd BLAST		135
Domainⁱ	155 – 242	HPrPROSITE-ProRule annotation1 PublicationAdd BLAST		88

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	264 – 472	PTS EI-like, N-terminal part1 PublicationAdd BLAST		209

Domainⁱ

Consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. The IIA domain of DhaM (via phospho-His-9), instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). The phosphoryl flow likely involves HPr ('His-15') -> DhaM (His-430 -> His-169 -> His-9) -> DhaL-ADP -> Dha. The HPr-like domain of DhaM cannot efficiently substitute for the general carrier protein HPr.1 Publication

Sequence similaritiesⁱ

Belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105VDH Bacteria COG1080 LUCA COG1925 LUCA COG3412 LUCA
HOGENOMⁱ	HOG000119746
KEGG Orthology (KO) More...KOⁱ	K05881
OMAⁱ	TDHVLVM
PhylomeDBⁱ	P37349

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00367 PTS-HPr_like, 1 hit
Gene3Dⁱ	1.10.274.10, 1 hit 3.30.1340.10, 1 hit 3.40.50.510, 1 hit
InterProⁱ	View protein in InterPro IPR012844 DhaM_N IPR000032 HPr-like IPR035895 HPr-like_sf IPR008279 PEP-util_enz_mobile_dom IPR036637 Phosphohistidine_dom_sf IPR004701 PTS_EIIA_man-typ IPR036662 PTS_EIIA_man-typ_sf IPR008731 PTS_EIN IPR001020 PTS_HPr_His_P_site IPR036618 PtsI_HPr-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03610 EIIA-man, 1 hit PF05524 PEP-utilisers_N, 1 hit PF00391 PEP-utilizers, 1 hit PF00381 PTS-HPr, 1 hit
PRINTSⁱ	PR00107 PHOSPHOCPHPR
SUPFAMⁱ	SSF47831 SSF47831, 1 hit SSF52009 SSF52009, 1 hit SSF53062 SSF53062, 1 hit SSF55594 SSF55594, 1 hit
TIGRFAMsⁱ	TIGR02364 dha_pts, 1 hit TIGR01003 PTS_HPr_family, 1 hit
PROSITEⁱ	View protein in PROSITE PS51096 PTS_EIIA_TYPE_4, 1 hit PS51350 PTS_HPR_DOM, 1 hit PS00369 PTS_HPR_HIS, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P37349-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVNLVIVSHS SRLGEGVGEL ARQMLMSDSC KIAIAAGIDD PQNPIGTDAV 
        60         70         80         90        100
KVMEAIESVA DADHVLVMMD MGSALLSAET ALELLAPEIA AKVRLCAAPL 
       110        120        130        140        150
VEGTLAATVS AASGADIDKV IFDAMHALEA KREQLGLPSS DTEISDTCPA 
       160        170        180        190        200
YDEEARSLAV VIKNRNGLHV RPASRLVYTL STFNADMLLE KNGKCVTPES 
       210        220        230        240        250
INQIALLQVR YNDTLRLIAK GPEAEEALIA FRQLAEDNFG ETEEVAPPTL 
       260        270        280        290        300
RPVPPVSGKA FYYQPVLCTV QAKSTLTVEE EQDRLRQAID FTLLDLMTLT 
       310        320        330        340        350
AKAEASGLDD IAAIFSGHHT LLDDPELLAA ASELLQHEHC TAEYAWQQVL 
       360        370        380        390        400
KELSQQYQQL DDEYLQARYI DVDDLLHRTL VHLTQTKEEL PQFNSPTILL 
       410        420        430        440        450
AENIYPSTVL QLDPAVVKGI CLSAGSPVSH SALIARELGI GWICQQGEKL 
       460        470 
YAIQPEETLT LDVKTQRFNR QG

Length:472

Mass (Da):51,449

Last modified:November 24, 2009 - v3

Checksum:ⁱ7CEBEFBA1544C65E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74282.4 AP009048 Genomic DNA Translation: BAA36055.1 X15868 Genomic DNA No translation available.
PIRⁱ	C64866
NCBI Reference Sequences More...RefSeqⁱ	NP_415716.4, NC_000913.3 WP_001301101.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74282; AAC74282; b1198 BAA36055; BAA36055; BAA36055
GeneIDⁱ	945749
KEGGⁱ	ecj:JW5185 eco:b1198
PATRICⁱ	fig\|1411691.4.peg.1087

Protein	Similar proteins		Species	Score	Length	Source
P37349	Dihydroxyacetone kinase		ECOLX		472	UniRef100_P37349
Dihydroxyacetone kinase subunit M		Escherichia coli 541-15		472
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM		Escherichia coli 1-110-08_S3_C1		472
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM		Escherichia coli 2-210-07_S3_C3		472
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM		Escherichia coli MP021561.2		472
+22

Protein	Similar proteins		Species	Score	Length	Source
P37349	Dihydroxyacetone kinase		ECOLX		472	UniRef90_P37349
Dihydroxyacetone kinase subunit M		Escherichia coli 541-15		472
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM		Escherichia coli 1-110-08_S3_C1		472
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM		Escherichia coli 2-210-07_S3_C3		472
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM		Escherichia coli MP021561.2		472
+706

Protein	Similar proteins		Species	Score	Length	Source
P37349	PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM		PANAM		473	UniRef50_P37349
PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM		KLEOK		472
Dihydroxyacetone kinase		ECOLX		472
Dihydroxyacetone kinase subunit M		Escherichia coli 541-15		472
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit dhaM		Escherichia coli 1-110-08_S3_C1		472
+1566

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74282.4 AP009048 Genomic DNA Translation: BAA36055.1 X15868 Genomic DNA No translation available.
PIRⁱ	C64866
RefSeqⁱ	NP_415716.4, NC_000913.3 WP_001301101.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P37349
SMRⁱ	P37349
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260761, 22 interactors
ComplexPortalⁱ	CPX-2634 Dha Kinase
DIPⁱ	DIP-11551N
IntActⁱ	P37349, 7 interactors
STRINGⁱ	316385.ECDH10B_1251

2D gel databases

SWISS-2DPAGEⁱ	P37349

SWISS-2DPAGEⁱ

P37349

Proteomic databases

PaxDbⁱ	P37349
PRIDEⁱ	P37349

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74282; AAC74282; b1198 BAA36055; BAA36055; BAA36055
GeneIDⁱ	945749
KEGGⁱ	ecj:JW5185 eco:b1198
PATRICⁱ	fig\|1411691.4.peg.1087

Organism-specific databases

EchoBASEⁱ	EB2299
EcoGeneⁱ	EG12399 dhaM

Phylogenomic databases

eggNOGⁱ	ENOG4105VDH Bacteria COG1080 LUCA COG1925 LUCA COG3412 LUCA
HOGENOMⁱ	HOG000119746
KOⁱ	K05881
OMAⁱ	TDHVLVM
PhylomeDBⁱ	P37349

Enzyme and pathway databases

UniPathwayⁱ	UPA00616
BioCycⁱ	EcoCyc:EG12399-MONOMER MetaCyc:EG12399-MONOMER
BRENDAⁱ	2.7.1.29 2026

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P37349

PROⁱ

PR:P37349

Family and domain databases

CDDⁱ	cd00367 PTS-HPr_like, 1 hit
Gene3Dⁱ	1.10.274.10, 1 hit 3.30.1340.10, 1 hit 3.40.50.510, 1 hit
InterProⁱ	View protein in InterPro IPR012844 DhaM_N IPR000032 HPr-like IPR035895 HPr-like_sf IPR008279 PEP-util_enz_mobile_dom IPR036637 Phosphohistidine_dom_sf IPR004701 PTS_EIIA_man-typ IPR036662 PTS_EIIA_man-typ_sf IPR008731 PTS_EIN IPR001020 PTS_HPr_His_P_site IPR036618 PtsI_HPr-bd_sf
Pfamⁱ	View protein in Pfam PF03610 EIIA-man, 1 hit PF05524 PEP-utilisers_N, 1 hit PF00391 PEP-utilizers, 1 hit PF00381 PTS-HPr, 1 hit
PRINTSⁱ	PR00107 PHOSPHOCPHPR
SUPFAMⁱ	SSF47831 SSF47831, 1 hit SSF52009 SSF52009, 1 hit SSF53062 SSF53062, 1 hit SSF55594 SSF55594, 1 hit
TIGRFAMsⁱ	TIGR02364 dha_pts, 1 hit TIGR01003 PTS_HPr_family, 1 hit
PROSITEⁱ	View protein in PROSITE PS51096 PTS_EIIA_TYPE_4, 1 hit PS51350 PTS_HPR_DOM, 1 hit PS00369 PTS_HPR_HIS, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DHAM_ECOLI
Accessionⁱ	P37349Primary (citable) accession number: P37349 Secondary accession number(s): P76013
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
	Last sequence update:	November 24, 2009
	Last modified:	October 10, 2018
	This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P37349 (DHAM_ECOLI)

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PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Caution

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycerol degradation

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

Pathwayⁱ: glycerol degradation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ