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Protein

PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM

Gene

dhaM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.1 Publication

Miscellaneous

Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKLM has no transport activity.1 Publication

Caution

Was reported to be a protein deacetylase that removes acetyl groups on specific lysine residues in target proteins (PubMed:26716769). However, later experiments demonstrate that this protein does not have any protein deacetylase activity; the discrepancy observed seems to be due to contaminants having proteolytic activity (PubMed:29939131).2 Publications

Catalytic activityi

Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate.1 Publication

Kineticsi

kcat is 2.8 sec(-1). Values measured with the DhaKLM complex.1 Publication
  1. KM=45 µM for dihydroxyacetone1 Publication

    Pathwayi: glycerol degradation

    This protein is involved in the pathway glycerol degradation, which is part of Polyol metabolism.Curated
    View all proteins of this organism that are known to be involved in the pathway glycerol degradation and in Polyol metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei9Tele-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication1
    Active sitei169Pros-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication1
    Active sitei430Tele-phosphohistidine intermediate1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12399-MONOMER
    MetaCyc:EG12399-MONOMER
    BRENDAi2.7.1.29 2026
    UniPathwayi
    UPA00616

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM1 Publication (EC:2.7.1.1211 Publication)
    Alternative name(s):
    Dihydroxyacetone kinase subunit M1 Publication
    Gene namesi
    Name:dhaM1 Publication
    Synonyms:ycgC
    Ordered Locus Names:b1198, JW5185
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12399 dhaM

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi9H → A: Loss of phosphotransfer activity. 1 Publication1
    Mutagenesisi169H → A: Loss of phosphotransfer activity. 1 Publication1
    Mutagenesisi430H → A: Loss of phosphotransfer activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001867131 – 472PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaMAdd BLAST472

    Proteomic databases

    PaxDbiP37349
    PRIDEiP37349

    2D gel databases

    SWISS-2DPAGEiP37349

    Interactioni

    Subunit structurei

    Homodimer (PubMed:11350937). The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.1 Publication

    Protein-protein interaction databases

    BioGridi4260761, 22 interactors
    ComplexPortaliCPX-2634 Dha Kinase
    DIPiDIP-11551N
    IntActiP37349, 7 interactors
    STRINGi316385.ECDH10B_1251

    Structurei

    3D structure databases

    ProteinModelPortaliP37349
    SMRiP37349
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 135PTS EIIA type-4PROSITE-ProRule annotation1 PublicationAdd BLAST135
    Domaini155 – 242HPrPROSITE-ProRule annotation1 PublicationAdd BLAST88

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni264 – 472PTS EI-like, N-terminal part1 PublicationAdd BLAST209

    Domaini

    Consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. The IIA domain of DhaM (via phospho-His-9), instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). The phosphoryl flow likely involves HPr ('His-15') -> DhaM (His-430 -> His-169 -> His-9) -> DhaL-ADP -> Dha. The HPr-like domain of DhaM cannot efficiently substitute for the general carrier protein HPr.1 Publication

    Sequence similaritiesi

    Belongs to the PEP-utilizing enzyme family.Curated

    Phylogenomic databases

    eggNOGiENOG4105VDH Bacteria
    COG1080 LUCA
    COG1925 LUCA
    COG3412 LUCA
    HOGENOMiHOG000119746
    KOiK05881
    OMAiTDHVLVM
    PhylomeDBiP37349

    Family and domain databases

    CDDicd00367 PTS-HPr_like, 1 hit
    Gene3Di1.10.274.10, 1 hit
    3.30.1340.10, 1 hit
    3.40.50.510, 1 hit
    InterProiView protein in InterPro
    IPR012844 DhaM_N
    IPR000032 HPr-like
    IPR035895 HPr-like_sf
    IPR008279 PEP-util_enz_mobile_dom
    IPR036637 Phosphohistidine_dom_sf
    IPR004701 PTS_EIIA_man-typ
    IPR036662 PTS_EIIA_man-typ_sf
    IPR008731 PTS_EIN
    IPR001020 PTS_HPr_His_P_site
    IPR036618 PtsI_HPr-bd_sf
    PfamiView protein in Pfam
    PF03610 EIIA-man, 1 hit
    PF05524 PEP-utilisers_N, 1 hit
    PF00391 PEP-utilizers, 1 hit
    PF00381 PTS-HPr, 1 hit
    PRINTSiPR00107 PHOSPHOCPHPR
    SUPFAMiSSF47831 SSF47831, 1 hit
    SSF52009 SSF52009, 1 hit
    SSF53062 SSF53062, 1 hit
    SSF55594 SSF55594, 1 hit
    TIGRFAMsiTIGR02364 dha_pts, 1 hit
    TIGR01003 PTS_HPr_family, 1 hit
    PROSITEiView protein in PROSITE
    PS51096 PTS_EIIA_TYPE_4, 1 hit
    PS51350 PTS_HPR_DOM, 1 hit
    PS00369 PTS_HPR_HIS, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P37349-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVNLVIVSHS SRLGEGVGEL ARQMLMSDSC KIAIAAGIDD PQNPIGTDAV
    60 70 80 90 100
    KVMEAIESVA DADHVLVMMD MGSALLSAET ALELLAPEIA AKVRLCAAPL
    110 120 130 140 150
    VEGTLAATVS AASGADIDKV IFDAMHALEA KREQLGLPSS DTEISDTCPA
    160 170 180 190 200
    YDEEARSLAV VIKNRNGLHV RPASRLVYTL STFNADMLLE KNGKCVTPES
    210 220 230 240 250
    INQIALLQVR YNDTLRLIAK GPEAEEALIA FRQLAEDNFG ETEEVAPPTL
    260 270 280 290 300
    RPVPPVSGKA FYYQPVLCTV QAKSTLTVEE EQDRLRQAID FTLLDLMTLT
    310 320 330 340 350
    AKAEASGLDD IAAIFSGHHT LLDDPELLAA ASELLQHEHC TAEYAWQQVL
    360 370 380 390 400
    KELSQQYQQL DDEYLQARYI DVDDLLHRTL VHLTQTKEEL PQFNSPTILL
    410 420 430 440 450
    AENIYPSTVL QLDPAVVKGI CLSAGSPVSH SALIARELGI GWICQQGEKL
    460 470
    YAIQPEETLT LDVKTQRFNR QG
    Length:472
    Mass (Da):51,449
    Last modified:November 24, 2009 - v3
    Checksum:i7CEBEFBA1544C65E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74282.4
    AP009048 Genomic DNA Translation: BAA36055.1
    X15868 Genomic DNA No translation available.
    PIRiC64866
    RefSeqiNP_415716.4, NC_000913.3
    WP_001301101.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74282; AAC74282; b1198
    BAA36055; BAA36055; BAA36055
    GeneIDi945749
    KEGGiecj:JW5185
    eco:b1198
    PATRICifig|1411691.4.peg.1087

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC74282.4
    AP009048 Genomic DNA Translation: BAA36055.1
    X15868 Genomic DNA No translation available.
    PIRiC64866
    RefSeqiNP_415716.4, NC_000913.3
    WP_001301101.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP37349
    SMRiP37349
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260761, 22 interactors
    ComplexPortaliCPX-2634 Dha Kinase
    DIPiDIP-11551N
    IntActiP37349, 7 interactors
    STRINGi316385.ECDH10B_1251

    2D gel databases

    SWISS-2DPAGEiP37349

    Proteomic databases

    PaxDbiP37349
    PRIDEiP37349

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC74282; AAC74282; b1198
    BAA36055; BAA36055; BAA36055
    GeneIDi945749
    KEGGiecj:JW5185
    eco:b1198
    PATRICifig|1411691.4.peg.1087

    Organism-specific databases

    EchoBASEiEB2299
    EcoGeneiEG12399 dhaM

    Phylogenomic databases

    eggNOGiENOG4105VDH Bacteria
    COG1080 LUCA
    COG1925 LUCA
    COG3412 LUCA
    HOGENOMiHOG000119746
    KOiK05881
    OMAiTDHVLVM
    PhylomeDBiP37349

    Enzyme and pathway databases

    UniPathwayi
    UPA00616

    BioCyciEcoCyc:EG12399-MONOMER
    MetaCyc:EG12399-MONOMER
    BRENDAi2.7.1.29 2026

    Miscellaneous databases

    PROiPR:P37349

    Family and domain databases

    CDDicd00367 PTS-HPr_like, 1 hit
    Gene3Di1.10.274.10, 1 hit
    3.30.1340.10, 1 hit
    3.40.50.510, 1 hit
    InterProiView protein in InterPro
    IPR012844 DhaM_N
    IPR000032 HPr-like
    IPR035895 HPr-like_sf
    IPR008279 PEP-util_enz_mobile_dom
    IPR036637 Phosphohistidine_dom_sf
    IPR004701 PTS_EIIA_man-typ
    IPR036662 PTS_EIIA_man-typ_sf
    IPR008731 PTS_EIN
    IPR001020 PTS_HPr_His_P_site
    IPR036618 PtsI_HPr-bd_sf
    PfamiView protein in Pfam
    PF03610 EIIA-man, 1 hit
    PF05524 PEP-utilisers_N, 1 hit
    PF00391 PEP-utilizers, 1 hit
    PF00381 PTS-HPr, 1 hit
    PRINTSiPR00107 PHOSPHOCPHPR
    SUPFAMiSSF47831 SSF47831, 1 hit
    SSF52009 SSF52009, 1 hit
    SSF53062 SSF53062, 1 hit
    SSF55594 SSF55594, 1 hit
    TIGRFAMsiTIGR02364 dha_pts, 1 hit
    TIGR01003 PTS_HPr_family, 1 hit
    PROSITEiView protein in PROSITE
    PS51096 PTS_EIIA_TYPE_4, 1 hit
    PS51350 PTS_HPR_DOM, 1 hit
    PS00369 PTS_HPR_HIS, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDHAM_ECOLI
    AccessioniPrimary (citable) accession number: P37349
    Secondary accession number(s): P76013
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: November 24, 2009
    Last modified: October 10, 2018
    This is version 160 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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    Main funding by: National Institutes of Health

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