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UniProtKB - P37349 (DHAM_ECOLI)
Protein
PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM
Gene
dhaM
Organism
Escherichia coli (strain K12)
Status
Functioni
Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.
1 PublicationMiscellaneous
Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKLM has no transport activity.1 Publication
Caution
Was reported to be a protein deacetylase that removes acetyl groups on specific lysine residues in target proteins (PubMed:26716769). However, later experiments demonstrate that this protein does not have any protein deacetylase activity; the discrepancy observed seems to be due to contaminants having proteolytic activity (PubMed:29939131).2 Publications
Catalytic activityi
- EC:2.7.1.1211 Publication
Kineticsi
kcat is 2.8 sec(-1). Values measured with the DhaKLM complex.1 Publication
- KM=45 µM for dihydroxyacetone1 Publication
: glycerol degradation Pathwayi
This protein is involved in the pathway glycerol degradation, which is part of Polyol metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway glycerol degradation and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 9 | Tele-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 169 | Pros-phosphohistidine intermediatePROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 430 | Tele-phosphohistidine intermediate1 Publication | 1 |
GO - Molecular functioni
- phosphoenolpyruvate-glycerone phosphotransferase activity Source: UniProtKB
GO - Biological processi
- carbohydrate phosphorylation Source: ComplexPortal
- cellular response to DNA damage stimulus Source: EcoliWiki
- glycerol catabolic process Source: UniProtKB-UniPathway
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: InterPro
- pyruvate metabolic process Source: ComplexPortal
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12399-MONOMER |
BRENDAi | 2.7.1.29, 2026 |
SABIO-RKi | P37349 |
UniPathwayi | UPA00616 |
Names & Taxonomyi
Protein namesi | Recommended name: PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM1 Publication (EC:2.7.1.1211 Publication)Alternative name(s): Dihydroxyacetone kinase subunit M1 Publication |
Gene namesi | Name:dhaM1 Publication Synonyms:ycgC Ordered Locus Names:b1198, JW5185 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- integral component of membrane Source: InterPro
- transferase complex Source: ComplexPortal
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | H → A: Loss of phosphotransfer activity. 1 Publication | 1 | |
Mutagenesisi | 169 | H → A: Loss of phosphotransfer activity. 1 Publication | 1 | |
Mutagenesisi | 430 | H → A: Loss of phosphotransfer activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186713 | 1 – 472 | PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaMAdd BLAST | 472 |
Proteomic databases
jPOSTi | P37349 |
PaxDbi | P37349 |
PRIDEi | P37349 |
2D gel databases
SWISS-2DPAGEi | P37349 |
Interactioni
Subunit structurei
Homodimer (PubMed:11350937). The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260761, 22 interactors 850116, 1 interactor |
ComplexPortali | CPX-2634, Dha Kinase |
DIPi | DIP-11551N |
IntActi | P37349, 7 interactors |
STRINGi | 511145.b1198 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 135 | PTS EIIA type-4PROSITE-ProRule annotation1 PublicationAdd BLAST | 135 | |
Domaini | 155 – 242 | HPrPROSITE-ProRule annotation1 PublicationAdd BLAST | 88 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 264 – 472 | PTS EI-like, N-terminal part1 PublicationAdd BLAST | 209 |
Domaini
Consists of three domains. The N-terminal dimerization domain has the same fold as the IIA domain of the mannose transporter of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS). The middle domain is similar to HPr and the C-terminus is similar to the N-terminal domain of enzyme I (EI) of the PTS. The IIA domain of DhaM (via phospho-His-9), instead of ATP, is the phosphoryl donor to dihydroxyacetone (Dha). The phosphoryl flow likely involves HPr ('His-15') -> DhaM (His-430 -> His-169 -> His-9) -> DhaL-ADP -> Dha. The HPr-like domain of DhaM cannot efficiently substitute for the general carrier protein HPr.1 Publication
Sequence similaritiesi
Belongs to the PEP-utilizing enzyme family.Curated
Phylogenomic databases
eggNOGi | COG1080, Bacteria COG1925, Bacteria COG3412, Bacteria |
OMAi | TDHVLVM |
PhylomeDBi | P37349 |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit |
Gene3Di | 1.10.274.10, 1 hit 3.30.1340.10, 1 hit 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR000032, HPr-like IPR035895, HPr-like_sf IPR008279, PEP-util_enz_mobile_dom IPR036637, Phosphohistidine_dom_sf IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf IPR008731, PTS_EIN IPR001020, PTS_HPr_His_P_site IPR036618, PtsI_HPr-bd_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF00381, PTS-HPr, 1 hit |
PRINTSi | PR00107, PHOSPHOCPHPR |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF52009, SSF52009, 1 hit SSF53062, SSF53062, 1 hit SSF55594, SSF55594, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit TIGR01003, PTS_HPr_family, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit PS51350, PTS_HPR_DOM, 1 hit PS00369, PTS_HPR_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
P37349-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVNLVIVSHS SRLGEGVGEL ARQMLMSDSC KIAIAAGIDD PQNPIGTDAV
60 70 80 90 100
KVMEAIESVA DADHVLVMMD MGSALLSAET ALELLAPEIA AKVRLCAAPL
110 120 130 140 150
VEGTLAATVS AASGADIDKV IFDAMHALEA KREQLGLPSS DTEISDTCPA
160 170 180 190 200
YDEEARSLAV VIKNRNGLHV RPASRLVYTL STFNADMLLE KNGKCVTPES
210 220 230 240 250
INQIALLQVR YNDTLRLIAK GPEAEEALIA FRQLAEDNFG ETEEVAPPTL
260 270 280 290 300
RPVPPVSGKA FYYQPVLCTV QAKSTLTVEE EQDRLRQAID FTLLDLMTLT
310 320 330 340 350
AKAEASGLDD IAAIFSGHHT LLDDPELLAA ASELLQHEHC TAEYAWQQVL
360 370 380 390 400
KELSQQYQQL DDEYLQARYI DVDDLLHRTL VHLTQTKEEL PQFNSPTILL
410 420 430 440 450
AENIYPSTVL QLDPAVVKGI CLSAGSPVSH SALIARELGI GWICQQGEKL
460 470
YAIQPEETLT LDVKTQRFNR QG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74282.4 AP009048 Genomic DNA Translation: BAA36055.1 X15868 Genomic DNA No translation available. |
PIRi | C64866 |
RefSeqi | NP_415716.4, NC_000913.3 WP_001301101.1, NZ_SSZK01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC74282; AAC74282; b1198 BAA36055; BAA36055; BAA36055 |
GeneIDi | 945749 |
KEGGi | ecj:JW5185 eco:b1198 |
PATRICi | fig|1411691.4.peg.1087 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74282.4 AP009048 Genomic DNA Translation: BAA36055.1 X15868 Genomic DNA No translation available. |
PIRi | C64866 |
RefSeqi | NP_415716.4, NC_000913.3 WP_001301101.1, NZ_SSZK01000010.1 |
3D structure databases
AlphaFoldDBi | P37349 |
SMRi | P37349 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260761, 22 interactors 850116, 1 interactor |
ComplexPortali | CPX-2634, Dha Kinase |
DIPi | DIP-11551N |
IntActi | P37349, 7 interactors |
STRINGi | 511145.b1198 |
2D gel databases
SWISS-2DPAGEi | P37349 |
Proteomic databases
jPOSTi | P37349 |
PaxDbi | P37349 |
PRIDEi | P37349 |
Genome annotation databases
EnsemblBacteriai | AAC74282; AAC74282; b1198 BAA36055; BAA36055; BAA36055 |
GeneIDi | 945749 |
KEGGi | ecj:JW5185 eco:b1198 |
PATRICi | fig|1411691.4.peg.1087 |
Organism-specific databases
EchoBASEi | EB2299 |
Phylogenomic databases
eggNOGi | COG1080, Bacteria COG1925, Bacteria COG3412, Bacteria |
OMAi | TDHVLVM |
PhylomeDBi | P37349 |
Enzyme and pathway databases
UniPathwayi | UPA00616 |
BioCyci | EcoCyc:EG12399-MONOMER |
BRENDAi | 2.7.1.29, 2026 |
SABIO-RKi | P37349 |
Miscellaneous databases
PROi | PR:P37349 |
Family and domain databases
CDDi | cd00367, PTS-HPr_like, 1 hit |
Gene3Di | 1.10.274.10, 1 hit 3.30.1340.10, 1 hit 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR000032, HPr-like IPR035895, HPr-like_sf IPR008279, PEP-util_enz_mobile_dom IPR036637, Phosphohistidine_dom_sf IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf IPR008731, PTS_EIN IPR001020, PTS_HPr_His_P_site IPR036618, PtsI_HPr-bd_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit PF05524, PEP-utilisers_N, 1 hit PF00391, PEP-utilizers, 1 hit PF00381, PTS-HPr, 1 hit |
PRINTSi | PR00107, PHOSPHOCPHPR |
SUPFAMi | SSF47831, SSF47831, 1 hit SSF52009, SSF52009, 1 hit SSF53062, SSF53062, 1 hit SSF55594, SSF55594, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit TIGR01003, PTS_HPr_family, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit PS51350, PTS_HPR_DOM, 1 hit PS00369, PTS_HPR_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHAM_ECOLI | |
Accessioni | P37349Primary (citable) accession number: P37349 Secondary accession number(s): P76013 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 24, 2009 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families