UniProtKB - P37340 (MDTK_ECOLI)
Protein
Multidrug resistance protein MdtK
Gene
mdtK
Organism
Escherichia coli (strain K12)
Status
Functioni
Multidrug efflux pump that probably functions as a Na+/drug antiporter. Confers resistance to many drugs such as fluoroquinolones (norfloxacin, ciprofloxacin, enoxacin) and tetraphenylphosphonium ion (TPP) (PubMed:9661020, PubMed:11566977). Also to deoxycholate, doxorubicin, trimethoprim, chloramphenicol, fosfomycin, ethidium bromide and benzalkonium (PubMed:11566977). Also able to export peptides; when overexpressed, allows cells deleted for multiple peptidases (pepA, pepB, pepD and pepN) to grow in the presence of dipeptides Ala-Gln or Gly-Tyr which otherwise inhibit growth (PubMed:20067529). Cells overexpressing this protein have decreased intracellular levels of Ala-Gln dipeptide, and in a system that produces the Ala-Gln dipeptide overproduction of this protein increases export of the dipeptide (PubMed:20067529).4 Publications
GO - Molecular functioni
- dipeptide transmembrane transporter activity Source: EcoCyc
- solute:cation antiporter activity Source: EcoCyc
- transmembrane transporter activity Source: GO_Central
- xenobiotic transmembrane transporter activity Source: EcoliWiki
GO - Biological processi
- cellular response to reactive oxygen species Source: EcoCyc
- dipeptide transmembrane transport Source: EcoCyc
- drug transmembrane transport Source: UniProtKB-UniRule
- protein transport Source: UniProtKB-KW
- response to antibiotic Source: EcoCyc
- sodium ion transport Source: UniProtKB-UniRule
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: EcoCyc
- xenobiotic transport Source: EcoCyc
Keywordsi
Biological process | Antibiotic resistance, Antiport, Ion transport, Peptide transport, Protein transport, Sodium transport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
BioCyci | EcoCyc:YDHE-MONOMER MetaCyc:YDHE-MONOMER |
Protein family/group databases
TCDBi | 2.A.66.1.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug resistance protein MdtKAlternative name(s): Multidrug-efflux transporter |
Gene namesi | Name:mdtK Synonyms:norE1 Publication, norM1 Publication, ydhE Ordered Locus Names:b1663, JW1655 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 10 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 11 – 31 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 32 – 52 | PeriplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 53 – 73 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 74 – 92 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 93 – 113 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 114 – 126 | PeriplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 127 – 147 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 148 – 159 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 160 – 180 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 181 – 188 | PeriplasmicSequence analysis | 8 | |
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 210 – 242 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 243 – 263 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 264 – 275 | PeriplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 276 – 296 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 297 – 313 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 314 – 334 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 335 – 349 | PeriplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 350 – 370 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 371 – 386 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 387 – 407 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 408 – 417 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 418 – 438 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 439 – 457 | Cytoplasmic1 PublicationAdd BLAST | 19 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000164181 | 1 – 457 | Multidrug resistance protein MdtKAdd BLAST | 457 |
Proteomic databases
jPOSTi | P37340 |
PaxDbi | P37340 |
PRIDEi | P37340 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260264, 205 interactors |
STRINGi | 511145.b1663 |
Chemistry databases
BindingDBi | P37340 |
Family & Domainsi
Sequence similaritiesi
Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0534, Bacteria |
HOGENOMi | CLU_012893_6_0_6 |
InParanoidi | P37340 |
PhylomeDBi | P37340 |
Family and domain databases
HAMAPi | MF_00400, MdtK, 1 hit |
InterProi | View protein in InterPro IPR002528, MATE_fam IPR022913, Multidrug-R_MdtK |
Pfami | View protein in Pfam PF01554, MatE, 2 hits |
PIRSFi | PIRSF006603, DinF, 1 hit |
TIGRFAMsi | TIGR00797, matE, 1 hit |
i Sequence
Sequence statusi: Complete.
P37340-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQKYISEARL LLALAIPVIL AQIAQTAMGF VDTVMAGGYS ATDMAAVAIG
60 70 80 90 100
TSIWLPAILF GHGLLLALTP VIAQLNGSGR RERIAHQVRQ GFWLAGFVSV
110 120 130 140 150
LIMLVLWNAG YIIRSMENID PALADKAVGY LRALLWGAPG YLFFQVARNQ
160 170 180 190 200
CEGLAKTKPG MVMGFIGLLV NIPVNYIFIY GHFGMPELGG VGCGVATAAV
210 220 230 240 250
YWVMFLAMVS YIKRARSMRD IRNEKGTAKP DPAVMKRLIQ LGLPIALALF
260 270 280 290 300
FEVTLFAVVA LLVSPLGIVD VAGHQIALNF SSLMFVLPMS LAAAVTIRVG
310 320 330 340 350
YRLGQGSTLD AQTAARTGLM VGVCMATLTA IFTVSLREQI ALLYNDNPEV
360 370 380 390 400
VTLAAHLMLL AAVYQISDSI QVIGSGILRG YKDTRSIFYI TFTAYWVLGL
410 420 430 440 450
PSGYILALTD LVVEPMGPAG FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI
ILQRASR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 32 | D → S in AAB47941 (PubMed:9023191).Curated | 1 | |
Sequence conflicti | 100 | V → A in X69109 (Ref. 6) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U68703 Genomic DNA Translation: AAB47941.1 U00096 Genomic DNA Translation: AAT48136.1 AP009048 Genomic DNA Translation: BAA15430.1 X69109 Genomic DNA No translation available. |
PIRi | A64924 |
RefSeqi | WP_001174940.1, NZ_SSZK01000001.1 YP_025307.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48136; AAT48136; b1663 BAA15430; BAA15430; BAA15430 |
GeneIDi | 57731059 945883 |
KEGGi | ecj:JW1655 eco:b1663 |
PATRICi | fig|1411691.4.peg.594 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U68703 Genomic DNA Translation: AAB47941.1 U00096 Genomic DNA Translation: AAT48136.1 AP009048 Genomic DNA Translation: BAA15430.1 X69109 Genomic DNA No translation available. |
PIRi | A64924 |
RefSeqi | WP_001174940.1, NZ_SSZK01000001.1 YP_025307.1, NC_000913.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260264, 205 interactors |
STRINGi | 511145.b1663 |
Chemistry databases
BindingDBi | P37340 |
ChEMBLi | CHEMBL1681613 |
DrugCentrali | P37340 |
Protein family/group databases
TCDBi | 2.A.66.1.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily |
Proteomic databases
jPOSTi | P37340 |
PaxDbi | P37340 |
PRIDEi | P37340 |
Genome annotation databases
EnsemblBacteriai | AAT48136; AAT48136; b1663 BAA15430; BAA15430; BAA15430 |
GeneIDi | 57731059 945883 |
KEGGi | ecj:JW1655 eco:b1663 |
PATRICi | fig|1411691.4.peg.594 |
Organism-specific databases
EchoBASEi | EB2300 |
Phylogenomic databases
eggNOGi | COG0534, Bacteria |
HOGENOMi | CLU_012893_6_0_6 |
InParanoidi | P37340 |
PhylomeDBi | P37340 |
Enzyme and pathway databases
BioCyci | EcoCyc:YDHE-MONOMER MetaCyc:YDHE-MONOMER |
Miscellaneous databases
PROi | PR:P37340 |
Family and domain databases
HAMAPi | MF_00400, MdtK, 1 hit |
InterProi | View protein in InterPro IPR002528, MATE_fam IPR022913, Multidrug-R_MdtK |
Pfami | View protein in Pfam PF01554, MatE, 2 hits |
PIRSFi | PIRSF006603, DinF, 1 hit |
TIGRFAMsi | TIGR00797, matE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MDTK_ECOLI | |
Accessioni | P37340Primary (citable) accession number: P37340 Secondary accession number(s): P77276, P77765 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families