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UniProtKB - P37340 (MDTK_ECOLI)

Entry version 162 (02 Jun 2021)
Sequence version 2 (01 Nov 1997)
Previous versions | rss
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Protein

Multidrug resistance protein MdtK

Gene

mdtK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Multidrug efflux pump that probably functions as a Na+/drug antiporter. Confers resistance to many drugs such as fluoroquinolones (norfloxacin, ciprofloxacin, enoxacin) and tetraphenylphosphonium ion (TPP) (PubMed:9661020, PubMed:11566977).

Also to deoxycholate, doxorubicin, trimethoprim, chloramphenicol, fosfomycin, ethidium bromide and benzalkonium (PubMed:11566977).

Also able to export peptides; when overexpressed, allows cells deleted for multiple peptidases (pepA, pepB, pepD and pepN) to grow in the presence of dipeptides Ala-Gln or Gly-Tyr which otherwise inhibit growth (PubMed:20067529).

Cells overexpressing this protein have decreased intracellular levels of Ala-Gln dipeptide, and in a system that produces the Ala-Gln dipeptide overproduction of this protein increases export of the dipeptide (PubMed:20067529).

4 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAntibiotic resistance, Antiport, Ion transport, Peptide transport, Protein transport, Sodium transport, Transport
LigandSodium

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:YDHE-MONOMER
MetaCyc:YDHE-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.66.1.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Multidrug resistance protein MdtK
Alternative name(s):
Multidrug-efflux transporter
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:mdtK
Synonyms:norE1 Publication, norM1 Publication, ydhE
Ordered Locus Names:b1663, JW1655
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 10CytoplasmicSequence analysis10
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Topological domaini32 – 52PeriplasmicSequence analysisAdd BLAST21
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 92CytoplasmicSequence analysisAdd BLAST19
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 126PeriplasmicSequence analysisAdd BLAST13
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 159CytoplasmicSequence analysisAdd BLAST12
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 188PeriplasmicSequence analysis8
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210 – 242CytoplasmicSequence analysisAdd BLAST33
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 275PeriplasmicSequence analysisAdd BLAST12
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 313CytoplasmicSequence analysisAdd BLAST17
Transmembranei314 – 334HelicalSequence analysisAdd BLAST21
Topological domaini335 – 349PeriplasmicSequence analysisAdd BLAST15
Transmembranei350 – 370HelicalSequence analysisAdd BLAST21
Topological domaini371 – 386CytoplasmicSequence analysisAdd BLAST16
Transmembranei387 – 407HelicalSequence analysisAdd BLAST21
Topological domaini408 – 417PeriplasmicSequence analysis10
Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 457Cytoplasmic1 PublicationAdd BLAST19

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL1681613

DrugCentral

More...DrugCentrali		P37340

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001641811 – 457Multidrug resistance protein MdtKAdd BLAST457

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P37340

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P37340

PRoteomics IDEntifications database

More...PRIDEi		P37340

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260264, 205 interactors

STRING: functional protein association networks

More...STRINGi		511145.b1663

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P37340

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0534, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_012893_6_0_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P37340

Database for complete collections of gene phylogenies

More...PhylomeDBi		P37340

Family and domain databases

HAMAP database of protein families

More...HAMAPi		MF_00400, MdtK, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR002528, MATE_fam
IPR022913, Multidrug-R_MdtK

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01554, MatE, 2 hits

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF006603, DinF, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00797, matE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P37340-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQKYISEARL LLALAIPVIL AQIAQTAMGF VDTVMAGGYS ATDMAAVAIG 
        60         70         80         90        100
TSIWLPAILF GHGLLLALTP VIAQLNGSGR RERIAHQVRQ GFWLAGFVSV 
       110        120        130        140        150
LIMLVLWNAG YIIRSMENID PALADKAVGY LRALLWGAPG YLFFQVARNQ 
       160        170        180        190        200
CEGLAKTKPG MVMGFIGLLV NIPVNYIFIY GHFGMPELGG VGCGVATAAV 
       210        220        230        240        250
YWVMFLAMVS YIKRARSMRD IRNEKGTAKP DPAVMKRLIQ LGLPIALALF 
       260        270        280        290        300
FEVTLFAVVA LLVSPLGIVD VAGHQIALNF SSLMFVLPMS LAAAVTIRVG 
       310        320        330        340        350
YRLGQGSTLD AQTAARTGLM VGVCMATLTA IFTVSLREQI ALLYNDNPEV 
       360        370        380        390        400
VTLAAHLMLL AAVYQISDSI QVIGSGILRG YKDTRSIFYI TFTAYWVLGL 
       410        420        430        440        450
PSGYILALTD LVVEPMGPAG FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI 

ILQRASR
Length:457
Mass (Da):49,447
Last modified:November 1, 1997 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i33A60FC42ED6CFD7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti32D → S in AAB47941 (PubMed:9023191).Curated1
Sequence conflicti100V → A in X69109 (Ref. 6) Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U68703 Genomic DNA Translation: AAB47941.1
U00096 Genomic DNA Translation: AAT48136.1
AP009048 Genomic DNA Translation: BAA15430.1
X69109 Genomic DNA No translation available.

Protein sequence database of the Protein Information Resource

More...PIRi		A64924

NCBI Reference Sequences

More...RefSeqi		WP_001174940.1, NZ_SSZK01000001.1
YP_025307.1, NC_000913.3

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAT48136; AAT48136; b1663
BAA15430; BAA15430; BAA15430

Database of genes from NCBI RefSeq genomes

More...GeneIDi		945883

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW1655
eco:b1663

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.594

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68703 Genomic DNA Translation: AAB47941.1
U00096 Genomic DNA Translation: AAT48136.1
AP009048 Genomic DNA Translation: BAA15430.1
X69109 Genomic DNA No translation available.
PIRiA64924
RefSeqiWP_001174940.1, NZ_SSZK01000001.1
YP_025307.1, NC_000913.3

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4260264, 205 interactors
STRINGi511145.b1663

Chemistry databases

BindingDBiP37340
ChEMBLiCHEMBL1681613
DrugCentraliP37340

Protein family/group databases

TCDBi2.A.66.1.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

Proteomic databases

jPOSTiP37340
PaxDbiP37340
PRIDEiP37340

Genome annotation databases

EnsemblBacteriaiAAT48136; AAT48136; b1663
BAA15430; BAA15430; BAA15430
GeneIDi945883
KEGGiecj:JW1655
eco:b1663
PATRICifig|1411691.4.peg.594

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2300

Phylogenomic databases

eggNOGiCOG0534, Bacteria
HOGENOMiCLU_012893_6_0_6
InParanoidiP37340
PhylomeDBiP37340

Enzyme and pathway databases

BioCyciEcoCyc:YDHE-MONOMER
MetaCyc:YDHE-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P37340

Family and domain databases

HAMAPiMF_00400, MdtK, 1 hit
InterProiView protein in InterPro
IPR002528, MATE_fam
IPR022913, Multidrug-R_MdtK
PfamiView protein in Pfam
PF01554, MatE, 2 hits
PIRSFiPIRSF006603, DinF, 1 hit
TIGRFAMsiTIGR00797, matE, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMDTK_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P37340
Secondary accession number(s): P77276, P77765
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: June 2, 2021
This is version 162 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  2. SIMILARITY comments
    Index of protein domains and families
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