UniProtKB - P37339 (LHGD_ECOLI)
Protein
L-2-hydroxyglutarate dehydrogenase
Gene
lhgD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the dehydrogenation of L-2-hydroxyglutarate (L2HG) to alpha-ketoglutarate and couples to the respiratory chain by feeding electrons from the reaction into the membrane quinone pool. Functions in a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate.1 Publication
Caution
Was originally thought to be an oxidase, i.e. using molecular oxygen for oxidation of L-2-hydroxyglutarate and producing hydrogen peroxide. However, no O2 consumption could be detected with L2HG using the purified recombinant and active enzyme (PubMed:30498244).1 Publication1 Publication
Catalytic activityi
Cofactori
FADUniRule annotation2 Publications
: Amino-acid degradation Pathwayi
This protein is involved in Amino-acid degradation.1 PublicationView all proteins of this organism that are known to be involved in Amino-acid degradation.
GO - Molecular functioni
- (S)-2-hydroxy-acid oxidase activity Source: GO_Central
- 2-hydroxyglutarate dehydrogenase activity Source: EcoCyc
- flavin adenine dinucleotide binding Source: EcoCyc
- L-2-hydroxyglutarate oxidase activity Source: EcoliWiki
- oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor Source: UniProtKB-UniRule
GO - Biological processi
- L-lysine catabolic process Source: UniProtKB-UniRule
- lysine catabolic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12387-MONOMER MetaCyc:EG12387-MONOMER |
SABIO-RKi | P37339 |
Names & Taxonomyi
Protein namesi | Recommended name: L-2-hydroxyglutarate dehydrogenase1 Publication (EC:1.1.5.131 Publication)Short name: L2HG dehydrogenase1 Publication Alternative name(s): L2HG:quinone oxidoreductase1 Publication |
Gene namesi | Name:lhgD1 Publication Synonyms:lhgO1 Publication, ygaF Ordered Locus Names:b2660, JW2635 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication
GO - Cellular componenti
- cytoplasm Source: EcoliWiki
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169295 | 1 – 422 | L-2-hydroxyglutarate dehydrogenaseAdd BLAST | 422 |
Proteomic databases
jPOSTi | P37339 |
PaxDbi | P37339 |
PRIDEi | P37339 |
Expressioni
Inductioni
Expression is induced by RpoS during carbon starvation and at stationary phase. Is also regulated by cAMP-CRP. Repressed by CsiR. Makes part of the operon glaH-lhgD-gabDTP.1 Publication
Interactioni
Binary interactionsi
P37339
With | #Exp. | IntAct |
---|---|---|
ppiC [P0A9L5] | 3 | EBI-555990,EBI-555953 |
Protein-protein interaction databases
BioGRIDi | 4263106, 11 interactors 852377, 1 interactor |
DIPi | DIP-12096N |
IntActi | P37339, 4 interactors |
STRINGi | 511145.b2660 |
Family & Domainsi
Sequence similaritiesi
Belongs to the L2HGDH family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0579, Bacteria |
HOGENOMi | CLU_024775_0_1_6 |
InParanoidi | P37339 |
PhylomeDBi | P37339 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00990, L_hydroxyglutarate_dehydrogenase, 1 hit |
InterProi | View protein in InterPro IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR030862, L2HG_DH |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
P37339-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYDFVIIGGG IIGMSTAMQL IDVYPDARIA LLEKESAPAC HQTGHNSGVI
60 70 80 90 100
HAGVYYTPGS LKAQFCLAGN RATKAFCDQN GIRYDNCGKM LVATSDLEME
110 120 130 140 150
RMRALWERTA ANGIEREWLN ADELREREPN ITGLGGIFVP SSGIVSYRDV
160 170 180 190 200
TAAMAKIFQS RGGEIIYNAE VSGLNEHKNG VVIRTRQGGE YEASTLISCS
210 220 230 240 250
GLMADRLVKM LGLEPGFIIC PFRGEYFRLA PEHNQIVNHL IYPIPDPAMP
260 270 280 290 300
FLGVHLTRMI DGSVTVGPNA VLAFKREGYR KRDFSFSDTL EILGSSGIRR
310 320 330 340 350
VLQNHLRSGL GEMKNSLCKS GYLRLVQKYC PRLSLSDLQP WPAGVRAQAV
360 370 380 390 400
SPDGKLIDDF LFVTTPRTIH TCNAPSPAAT SAIPIGAHIV SKVQTLLASQ
410 420
SNPGRTLRAA RSVDALHAAF NQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75707.2 AP009048 Genomic DNA Translation: BAA16521.1 M88334 Genomic DNA No translation available. |
PIRi | E65045 |
RefSeqi | NP_417146.2, NC_000913.3 WP_000271909.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75707; AAC75707; b2660 BAA16521; BAA16521; BAA16521 |
GeneIDi | 948069 |
KEGGi | ecj:JW2635 eco:b2660 |
PATRICi | fig|1411691.4.peg.4081 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75707.2 AP009048 Genomic DNA Translation: BAA16521.1 M88334 Genomic DNA No translation available. |
PIRi | E65045 |
RefSeqi | NP_417146.2, NC_000913.3 WP_000271909.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4263106, 11 interactors 852377, 1 interactor |
DIPi | DIP-12096N |
IntActi | P37339, 4 interactors |
STRINGi | 511145.b2660 |
Proteomic databases
jPOSTi | P37339 |
PaxDbi | P37339 |
PRIDEi | P37339 |
Protocols and materials databases
DNASUi | 948069 |
Genome annotation databases
EnsemblBacteriai | AAC75707; AAC75707; b2660 BAA16521; BAA16521; BAA16521 |
GeneIDi | 948069 |
KEGGi | ecj:JW2635 eco:b2660 |
PATRICi | fig|1411691.4.peg.4081 |
Organism-specific databases
EchoBASEi | EB2288 |
Phylogenomic databases
eggNOGi | COG0579, Bacteria |
HOGENOMi | CLU_024775_0_1_6 |
InParanoidi | P37339 |
PhylomeDBi | P37339 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12387-MONOMER MetaCyc:EG12387-MONOMER |
SABIO-RKi | P37339 |
Miscellaneous databases
PROi | PR:P37339 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00990, L_hydroxyglutarate_dehydrogenase, 1 hit |
InterProi | View protein in InterPro IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR030862, L2HG_DH |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LHGD_ECOLI | |
Accessioni | P37339Primary (citable) accession number: P37339 Secondary accession number(s): P76622, P77020 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | December 4, 2007 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families