UniProtKB - P37338 (GLAR_ECOLI)
Protein
HTH-type transcriptional repressor GlaR
Gene
glaR
Organism
Escherichia coli (strain K12)
Status
Functioni
Negatively regulates the expression of the glaH-lhgD-gabDTP operon in a temporal manner during entry into stationary phase or during the first few hours of carbon starvation (PubMed:14731280, PubMed:30498244). Thereby is involved in the regulation of a L-lysine degradation pathway that proceeds via cadaverine, glutarate and L-2-hydroxyglutarate (PubMed:30498244). Binds to two primary and two secondary sites in the promoter region of the glaH operon with the consensus sequences TTGTN5TTTT and ATGTN5TTTT of the primary sites, each separated by six nucleotides (PubMed:30498244).2 Publications
Activity regulationi
The repressive effect at the glaH promoter site is specifically relieved upon glutarate binding.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 29 – 48 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: InterPro
GO - Biological processi
- negative regulation of DNA-templated transcription, initiation Source: EcoCyc
- regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12386-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional repressor GlaRAlternative name(s): Carbon starvation induced regulator GlaH operon repressor1 Publication |
Gene namesi | Name:glaR1 Publication Synonyms:csiR, gabC, ygaE Ordered Locus Names:b2664, JW2639 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050680 | 1 – 220 | HTH-type transcriptional repressor GlaRAdd BLAST | 220 |
Proteomic databases
jPOSTi | P37338 |
PaxDbi | P37338 |
PRIDEi | P37338 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262265, 4 interactors |
DIPi | DIP-12095N |
IntActi | P37338, 4 interactors |
STRINGi | 511145.b2664 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 69 | HTH gntR-typePROSITE-ProRule annotationAdd BLAST | 69 |
Phylogenomic databases
eggNOGi | COG1802, Bacteria |
HOGENOMi | CLU_017584_5_3_6 |
InParanoidi | P37338 |
PhylomeDBi | P37338 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.20.120.530, 1 hit |
InterProi | View protein in InterPro IPR011711, GntR_C IPR008920, TF_FadR/GntR_C IPR000524, Tscrpt_reg_HTH_GntR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF07729, FCD, 1 hit PF00392, GntR, 1 hit |
SMARTi | View protein in SMART SM00895, FCD, 1 hit SM00345, HTH_GNTR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF48008, SSF48008, 1 hit |
PROSITEi | View protein in PROSITE PS50949, HTH_GNTR, 1 hit |
i Sequence
Sequence statusi: Complete.
P37338-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTITSLDGYR WLKNDIIRGN FQPDEKLRMS LLTSRYALGV GPLREALSQL
60 70 80 90 100
VAERLVTVVN QKGYRVASMS EQELLDIFDA RANMEAMLVS LAIARGGDEW
110 120 130 140 150
EADVLAKAHL LSKLEACDAS EKMLDEWDLR HQAFHTAIVA GCGSHYLLQM
160 170 180 190 200
RERLFDLAAR YRFIWLRRTV LSVEMLEDKH DQHQTLTAAV LARDTARASE
210 220
LMRQHLLTPI PIIQQAMAGN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75711.2 AP009048 Genomic DNA Translation: BAA16527.1 M88334 Genomic DNA No translation available. |
RefSeqi | NP_417150.4, NC_000913.3 WP_000156811.1, NZ_STEB01000042.1 |
Genome annotation databases
EnsemblBacteriai | AAC75711; AAC75711; b2664 BAA16527; BAA16527; BAA16527 |
GeneIDi | 48726523 948055 |
KEGGi | ecj:JW2639 eco:b2664 |
PATRICi | fig|1411691.4.peg.4077 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75711.2 AP009048 Genomic DNA Translation: BAA16527.1 M88334 Genomic DNA No translation available. |
RefSeqi | NP_417150.4, NC_000913.3 WP_000156811.1, NZ_STEB01000042.1 |
3D structure databases
SMRi | P37338 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262265, 4 interactors |
DIPi | DIP-12095N |
IntActi | P37338, 4 interactors |
STRINGi | 511145.b2664 |
Proteomic databases
jPOSTi | P37338 |
PaxDbi | P37338 |
PRIDEi | P37338 |
Genome annotation databases
EnsemblBacteriai | AAC75711; AAC75711; b2664 BAA16527; BAA16527; BAA16527 |
GeneIDi | 48726523 948055 |
KEGGi | ecj:JW2639 eco:b2664 |
PATRICi | fig|1411691.4.peg.4077 |
Organism-specific databases
EchoBASEi | EB2287 |
Phylogenomic databases
eggNOGi | COG1802, Bacteria |
HOGENOMi | CLU_017584_5_3_6 |
InParanoidi | P37338 |
PhylomeDBi | P37338 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12386-MONOMER |
Miscellaneous databases
PROi | PR:P37338 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.20.120.530, 1 hit |
InterProi | View protein in InterPro IPR011711, GntR_C IPR008920, TF_FadR/GntR_C IPR000524, Tscrpt_reg_HTH_GntR IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF07729, FCD, 1 hit PF00392, GntR, 1 hit |
SMARTi | View protein in SMART SM00895, FCD, 1 hit SM00345, HTH_GNTR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF48008, SSF48008, 1 hit |
PROSITEi | View protein in PROSITE PS50949, HTH_GNTR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLAR_ECOLI | |
Accessioni | P37338Primary (citable) accession number: P37338 Secondary accession number(s): P76623, P77021 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |