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UniProtKB - P37330 (MASZ_ECOLI)
Protein
Malate synthase G
Gene
glcB
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.
UniRule annotation3 PublicationsCatalytic activityi
- EC:2.3.3.9UniRule annotation
Cofactori
Mg2+UniRule annotation1 Publication
Kineticsi
kcat is 48.1 sec(-1) for glyoxylate (at pH 8 and 37 degrees Celsius).
- KM=9 µM for acetyl-CoA (at pH 8 and 37 degrees Celsius)1 Publication
- KM=21 µM for glyoxylate (at pH 8 and 37 degrees Celsius)1 Publication
- Vmax=36.1 µmol/min/mg enzyme (at pH 8 and 37 degrees Celsius)1 Publication
: glyoxylate cycle Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | Acetyl-CoA; via carbonyl oxygen | 1 | |
Binding sitei | 274 | Acetyl-CoA | 1 | |
Binding sitei | 311 | Acetyl-CoA | 1 | |
Active sitei | 338 | Proton acceptor | 1 | |
Binding sitei | 338 | Glyoxylate | 1 | |
Metal bindingi | 427 | Magnesium | 1 | |
Binding sitei | 427 | Glyoxylate | 1 | |
Metal bindingi | 455 | Magnesium | 1 | |
Binding sitei | 536 | Acetyl-CoA; via carbonyl oxygen | 1 | |
Active sitei | 631 | Proton donor | 1 |
GO - Molecular functioni
- magnesium ion binding Source: GO_Central
- malate synthase activity Source: EcoCyc
GO - Biological processi
- glyoxylate catabolic process Source: EcoCyc
- glyoxylate cycle Source: UniProtKB-UniRule
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MALSYNG-MONOMER |
BRENDAi | 2.3.3.9, 2026 |
UniPathwayi | UPA00703;UER00720 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:glcBUniRule annotation Synonyms:glc Ordered Locus Names:b2976, JW2943 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene are unable to oxidize glyoxylate.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 338 | R → K: Has a specific activity which is only 6.6% of the wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 617 | C → S: Affinity binding for acetyl-CoA is more than five times greater than that of wild-type, although its specific activity is comparable. 1 Publication | 1 | |
Mutagenesisi | 631 | D → N: Absence of malate synthase activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB04343, Glyoxylic acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000166886 | 2 – 723 | Malate synthase GAdd BLAST | 722 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 617 | Cysteine sulfenic acid (-SOH)UniRule annotation1 Publication | 1 | |
Modified residuei | 688 | Cysteine sulfenic acid (-SOH)UniRule annotation1 Publication | 1 |
Keywords - PTMi
OxidationProteomic databases
jPOSTi | P37330 |
PaxDbi | P37330 |
PRIDEi | P37330 |
Expressioni
Inductioni
By glycolate. Part of the glcDEFGB operon, which is induced by growth on glycolate, under the positive control of GlcC. Also induced by growth on acetate. Expression of the glc operon is strongly dependent on the integration host factor (IHF) and is repressed by the global respiratory regulator ArcA-P (PubMed:9880556).3 Publications
Interactioni
Subunit structurei
Monomer.
UniRule annotation4 PublicationsProtein-protein interaction databases
BioGRIDi | 4259239, 20 interactors |
DIPi | DIP-9761N |
IntActi | P37330, 6 interactors |
MINTi | P37330 |
STRINGi | 511145.b2976 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P37330 |
SMRi | P37330 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37330 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 125 – 126 | Acetyl-CoA binding | 2 | |
Regioni | 452 – 455 | Glyoxylate binding | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2225, Bacteria |
HOGENOMi | CLU_028446_1_0_6 |
InParanoidi | P37330 |
PhylomeDBi | P37330 |
Family and domain databases
CDDi | cd00728, malate_synt_G, 1 hit |
DisProti | DP01837 |
Gene3Di | 1.20.1220.12, 1 hit |
HAMAPi | MF_00641, Malate_synth_G, 1 hit |
InterProi | View protein in InterPro IPR044856, Malate_synth_C_sf IPR011076, Malate_synth_sf IPR001465, Malate_synthase IPR006253, Malate_synthG |
PANTHERi | PTHR42739, PTHR42739, 1 hit |
Pfami | View protein in Pfam PF01274, Malate_synthase, 1 hit |
SUPFAMi | SSF51645, SSF51645, 1 hit |
TIGRFAMsi | TIGR01345, malate_syn_G, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P37330-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQTITQSRL RIDANFKRFV DEEVLPGTGL DAAAFWRNFD EIVHDLAPEN
60 70 80 90 100
RQLLAERDRI QAALDEWHRS NPGPVKDKAA YKSFLRELGY LVPQPERVTV
110 120 130 140 150
ETTGIDSEIT SQAGPQLVVP AMNARYALNA ANARWGSLYD ALYGSDIIPQ
160 170 180 190 200
EGAMVSGYDP QRGEQVIAWV RRFLDESLPL ENGSYQDVVA FKVVDKQLRI
210 220 230 240 250
QLKNGKETTL RTPAQFVGYR GDAAAPTCIL LKNNGLHIEL QIDANGRIGK
260 270 280 290 300
DDPAHINDVI VEAAISTILD CEDSVAAVDA EDKILLYRNL LGLMQGTLQE
310 320 330 340 350
KMEKNGRQIV RKLNDDRHYT AADGSEISLH GRSLLFIRNV GHLMTIPVIW
360 370 380 390 400
DSEGNEIPEG ILDGVMTGAI ALYDLKVQKN SRTGSVYIVK PKMHGPQEVA
410 420 430 440 450
FANKLFTRIE TMLGMAPNTL KMGIMDEERR TSLNLRSCIA QARNRVAFIN
460 470 480 490 500
TGFLDRTGDE MHSVMEAGPM LRKNQMKSTP WIKAYERNNV LSGLFCGLRG
510 520 530 540 550
KAQIGKGMWA MPDLMADMYS QKGDQLRAGA NTAWVPSPTA ATLHALHYHQ
560 570 580 590 600
TNVQSVQANI AQTEFNAEFE PLLDDLLTIP VAENANWSAQ EIQQELDNNV
610 620 630 640 650
QGILGYVVRW VEQGIGCSKV PDIHNVALME DRATLRISSQ HIANWLRHGI
660 670 680 690 700
LTKEQVQASL ENMAKVVDQQ NAGDPAYRPM AGNFANSCAF KAASDLIFLG
710 720
VKQPNGYTEP LLHAWRLREK ESH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74547 Genomic DNA Translation: CAA52639.1 U28377 Genomic DNA Translation: AAA69143.1 U00096 Genomic DNA Translation: AAC76012.1 AP009048 Genomic DNA Translation: BAE77036.1 |
PIRi | S51788 |
RefSeqi | NP_417450.1, NC_000913.3 WP_000084131.1, NZ_SSUV01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC76012; AAC76012; b2976 BAE77036; BAE77036; BAE77036 |
GeneIDi | 948857 |
KEGGi | ecj:JW2943 eco:b2976 |
PATRICi | fig|1411691.4.peg.3755 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74547 Genomic DNA Translation: CAA52639.1 U28377 Genomic DNA Translation: AAA69143.1 U00096 Genomic DNA Translation: AAC76012.1 AP009048 Genomic DNA Translation: BAE77036.1 |
PIRi | S51788 |
RefSeqi | NP_417450.1, NC_000913.3 WP_000084131.1, NZ_SSUV01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1D8C | X-ray | 2.00 | A | 1-723 | [»] | |
1P7T | X-ray | 1.95 | A/B | 3-723 | [»] | |
1Y8B | NMR | - | A | 1-723 | [»] | |
2JQX | NMR | - | A | 1-723 | [»] | |
BMRBi | P37330 | |||||
SMRi | P37330 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259239, 20 interactors |
DIPi | DIP-9761N |
IntActi | P37330, 6 interactors |
MINTi | P37330 |
STRINGi | 511145.b2976 |
Chemistry databases
DrugBanki | DB04343, Glyoxylic acid |
Proteomic databases
jPOSTi | P37330 |
PaxDbi | P37330 |
PRIDEi | P37330 |
Genome annotation databases
EnsemblBacteriai | AAC76012; AAC76012; b2976 BAE77036; BAE77036; BAE77036 |
GeneIDi | 948857 |
KEGGi | ecj:JW2943 eco:b2976 |
PATRICi | fig|1411691.4.peg.3755 |
Organism-specific databases
EchoBASEi | EB4141 |
Phylogenomic databases
eggNOGi | COG2225, Bacteria |
HOGENOMi | CLU_028446_1_0_6 |
InParanoidi | P37330 |
PhylomeDBi | P37330 |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00720 |
BioCyci | EcoCyc:MALSYNG-MONOMER |
BRENDAi | 2.3.3.9, 2026 |
Miscellaneous databases
EvolutionaryTracei | P37330 |
PROi | PR:P37330 |
Family and domain databases
CDDi | cd00728, malate_synt_G, 1 hit |
DisProti | DP01837 |
Gene3Di | 1.20.1220.12, 1 hit |
HAMAPi | MF_00641, Malate_synth_G, 1 hit |
InterProi | View protein in InterPro IPR044856, Malate_synth_C_sf IPR011076, Malate_synth_sf IPR001465, Malate_synthase IPR006253, Malate_synthG |
PANTHERi | PTHR42739, PTHR42739, 1 hit |
Pfami | View protein in Pfam PF01274, Malate_synthase, 1 hit |
SUPFAMi | SSF51645, SSF51645, 1 hit |
TIGRFAMsi | TIGR01345, malate_syn_G, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MASZ_ECOLI | |
Accessioni | P37330Primary (citable) accession number: P37330 Secondary accession number(s): Q2M9M0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 182 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families