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UniProtKB - P37253 (ILVC_BACSU)
Protein
Ketol-acid reductoisomerase (NADP(+))
Gene
ilvC
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
UniRule annotationCatalytic activityi
- EC:1.1.1.86UniRule annotation
- (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H+ + NADPHUniRule annotationEC:1.1.1.86UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation
: L-isoleucine biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: L-valine biosynthesis
This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | NADPUniRule annotation | 1 | |
Binding sitei | 52 | NADPUniRule annotation | 1 | |
Active sitei | 107 | UniRule annotation | 1 | |
Binding sitei | 133 | NADP; via amide nitrogenUniRule annotation | 1 | |
Metal bindingi | 190 | Magnesium 1UniRule annotation | 1 | |
Metal bindingi | 190 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 194 | Magnesium 1UniRule annotation | 1 | |
Metal bindingi | 226 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 230 | Magnesium 2UniRule annotation | 1 | |
Binding sitei | 251 | SubstrateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 25 – 28 | NADPUniRule annotation | 4 | |
Nucleotide bindingi | 82 – 85 | NADPUniRule annotation | 4 |
GO - Molecular functioni
- ketol-acid reductoisomerase activity Source: GO_Central
- magnesium ion binding Source: UniProtKB-UniRule
- NADP binding Source: InterPro
GO - Biological processi
- isoleucine biosynthetic process Source: GO_Central
- valine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Ligand | Magnesium, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | BSUB:BSU28290-MONOMER |
UniPathwayi | UPA00047;UER00056 UPA00049;UER00060 |
Names & Taxonomyi
Protein namesi | Recommended name: Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)Short name: KARIUniRule annotation Alternative name(s): Acetohydroxy-acid isomeroreductaseUniRule annotation Short name: AHIRUniRule annotation Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation Ketol-acid reductoisomerase type 1UniRule annotation Ketol-acid reductoisomerase type IUniRule annotation |
Gene namesi | Name:ilvCUniRule annotation Ordered Locus Names:BSU28290 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151277 | 1 – 342 | Ketol-acid reductoisomerase (NADP(+))Add BLAST | 342 |
Proteomic databases
PaxDbi | P37253 |
PRIDEi | P37253 |
Interactioni
Protein-protein interaction databases
IntActi | P37253, 1 interactor |
MINTi | P37253 |
STRINGi | 224308.BSU28290 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 181 | KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST | 180 | |
Domaini | 182 – 327 | KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST | 146 |
Sequence similaritiesi
Belongs to the ketol-acid reductoisomerase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0059, Bacteria |
InParanoidi | P37253 |
OMAi | RAMFSWL |
PhylomeDBi | P37253 |
Family and domain databases
HAMAPi | MF_00435, IlvC, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013023, KARI IPR000506, KARI_C IPR013116, KARI_N IPR014359, KARI_prok IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR21371, PTHR21371, 1 hit |
Pfami | View protein in Pfam PF01450, IlvC, 1 hit PF07991, IlvN, 1 hit |
PIRSFi | PIRSF000116, IlvC_gammaproteo, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00465, ilvC, 1 hit |
PROSITEi | View protein in PROSITE PS51851, KARI_C, 1 hit PS51850, KARI_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P37253-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKVYYNGDI KENVLAGKTV AVIGYGSQGH AHALNLKESG VDVIVGVRQG
60 70 80 90 100
KSFTQAQEDG HKVFSVKEAA AQAEIIMVLL PDEQQQKVYE AEIKDELTAG
110 120 130 140 150
KSLVFAHGFN VHFHQIVPPA DVDVFLVAPK GPGHLVRRTY EQGAGVPALF
160 170 180 190 200
AIYQDVTGEA RDKALAYAKG IGGARAGVLE TTFKEETETD LFGEQAVLCG
210 220 230 240 250
GLSALVKAGF ETLTEAGYQP ELAYFECLHE LKLIVDLMYE EGLAGMRYSI
260 270 280 290 300
SDTAQWGDFV SGPRVVDAKV KESMKEVLKD IQNGTFAKEW IVENQVNRPR
310 320 330 340
FNAINASENE HQIEVVGRKL REMMPFVKQG KKKEAVVSVA QN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L03181 Genomic DNA Translation: AAA22548.1 Z75208 Genomic DNA Translation: CAA99563.1 AL009126 Genomic DNA Translation: CAB14789.1 |
PIRi | C69644 |
RefSeqi | NP_390707.1, NC_000964.3 WP_003222549.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14789; CAB14789; BSU_28290 |
GeneIDi | 64304553 937475 |
KEGGi | bsu:BSU28290 |
PATRICi | fig|224308.179.peg.3073 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L03181 Genomic DNA Translation: AAA22548.1 Z75208 Genomic DNA Translation: CAA99563.1 AL009126 Genomic DNA Translation: CAB14789.1 |
PIRi | C69644 |
RefSeqi | NP_390707.1, NC_000964.3 WP_003222549.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | P37253 |
SMRi | P37253 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P37253, 1 interactor |
MINTi | P37253 |
STRINGi | 224308.BSU28290 |
Proteomic databases
PaxDbi | P37253 |
PRIDEi | P37253 |
Genome annotation databases
EnsemblBacteriai | CAB14789; CAB14789; BSU_28290 |
GeneIDi | 64304553 937475 |
KEGGi | bsu:BSU28290 |
PATRICi | fig|224308.179.peg.3073 |
Phylogenomic databases
eggNOGi | COG0059, Bacteria |
InParanoidi | P37253 |
OMAi | RAMFSWL |
PhylomeDBi | P37253 |
Enzyme and pathway databases
UniPathwayi | UPA00047;UER00056 UPA00049;UER00060 |
BioCyci | BSUB:BSU28290-MONOMER |
Family and domain databases
HAMAPi | MF_00435, IlvC, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013023, KARI IPR000506, KARI_C IPR013116, KARI_N IPR014359, KARI_prok IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR21371, PTHR21371, 1 hit |
Pfami | View protein in Pfam PF01450, IlvC, 1 hit PF07991, IlvN, 1 hit |
PIRSFi | PIRSF000116, IlvC_gammaproteo, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00465, ilvC, 1 hit |
PROSITEi | View protein in PROSITE PS51851, KARI_C, 1 hit PS51850, KARI_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ILVC_BACSU | |
Accessioni | P37253Primary (citable) accession number: P37253 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families