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Protein

Peroxisome proliferator-activated receptor gamma

Gene

Pparg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (PubMed:19041764).5 Publications

Activity regulationi

PDPK1 activates its transcriptional activity independently of its kinase activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi136 – 210Nuclear receptorPROSITE-ProRule annotationAdd BLAST75
Zinc fingeri139 – 159NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri176 – 198NR C4-typePROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Receptor
Biological processBiological rhythms, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Chemistry databases

SwissLipidsiSLP:000001625

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisome proliferator-activated receptor gamma
Short name:
PPAR-gamma
Alternative name(s):
Nuclear receptor subfamily 1 group C member 3
Gene namesi
Name:Pparg
Synonyms:Nr1c3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97747 Pparg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop abnormalities in circadian variations in blood pressure and heart rate, in parallel with a reduction of diurnal variations in the sympathetic nerve activity, and impaired rhythmicity of ARNTL/BMAL1 in the blood vessels.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112S → A: Increases basal and ligand-induced adipogenic activity. Abolishes repression by PER2 on transactivation activity. 2 Publications1
Mutagenesisi112S → D: No effect on repression by PER2 on transactivation activity. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL2459

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000534941 – 505Peroxisome proliferator-activated receptor gammaAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84O-linked (GlcNAc) threonine1 Publication1
Modified residuei112Phosphoserine; by MAPK2 Publications1

Post-translational modificationi

O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.1 Publication
Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated Ser-112 form is recognized by PER2 and repressed, dephosphorylation at Ser-112 induces adipogenic activity. Ser-112 phosphorylation levels are reduced by 65% in brown adipose tissue compared to white adipose tissue.2 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP37238
PRIDEiP37238

PTM databases

iPTMnetiP37238
PhosphoSitePlusiP37238

Expressioni

Tissue specificityi

Highest expression in white and brown adipose tissue. Also found in liver, skeletal muscle, heart, adrenal gland, spleen, kidney and intestine. Isoform 2 is more abundant than isoform 1 in adipose tissue.2 Publications

Developmental stagei

It appears first at 13.5 dpc and increases until birth.

Inductioni

Expressed in a circadian manner in the aorta.1 Publication

Gene expression databases

CleanExiMM_PPARG

Interactioni

Subunit structurei

Heterodimer with other nuclear receptors, such as RXRA. The heterodimer with the retinoic acid receptor RXRA is called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 and NCOA2 LXXLL motifs. Interacts with ASXL1, ASXL2, DNTTIP2, FAM120B, MAP2K1/MEK1, NR0B2, PDPK1, PRDM16, PRMT2 and TGFB1I1. Interacts (when activated by agonist) with PPP5C. Interacts with HELZ2 and THRAP3; the interaction stimulates the transcriptional activity of PPARG. Interacts with PER2, the interaction is ligand dependent and blocks PPARG recruitment to target promoters. Interacts with NOCT. Interacts with FOXO1 (acetylated form). Interacts with ACTN4 (By similarity). Interacts (when in the liganded conformation) with GPS2 (PubMed:25519902).By similarity13 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202320, 75 interactors
ComplexPortaliCPX-703 PPARgamma-NCOA2 activated nuclear receptor complex
CPX-864 PPARgamma-NCOA1 activated nuclear receptor complex
CORUMiP37238
DIPiDIP-60435N
ELMiP37238
IntActiP37238, 6 interactors
STRINGi10090.ENSMUSP00000000450

Chemistry databases

BindingDBiP37238

Structurei

3D structure databases

ProteinModelPortaliP37238
SMRiP37238
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini238 – 503NR LBDPROSITE-ProRule annotationAdd BLAST266

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 280Interaction with FAM120B1 PublicationAdd BLAST76

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri139 – 159NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri176 – 198NR C4-typePROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575 Eukaryota
ENOG410XRZC LUCA
HOGENOMiHOG000261626
HOVERGENiHBG106004
InParanoidiP37238
KOiK08530
PhylomeDBiP37238

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR003074 1Cnucl_rcpt
IPR035500 NHR_like_dom_sf
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR003077 PPAR-gamma
IPR022590 PPARgamma_N
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00104 Hormone_recep, 1 hit
PF12577 PPARgamma_N, 1 hit
PF00105 zf-C4, 1 hit
PRINTSiPR01288 PROXISOMEPAR
PR01291 PROXISOMPAGR
PR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 1 hit
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 2 (identifier: P37238-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGETLGDSPV DPEHGAFADA LPMSTSQEIT MVDTEMPFWP TNFGISSVDL
60 70 80 90 100
SVMEDHSHSF DIKPFTTVDF SSISAPHYED IPFTRADPMV ADYKYDLKLQ
110 120 130 140 150
EYQSAIKVEP ASPPYYSEKT QLYNRPHEEP SNSLMAIECR VCGDKASGFH
160 170 180 190 200
YGVHACEGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA
210 220 230 240 250
VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY
260 270 280 290 300
IKSFPLTKAK ARAILTGKTT DKSPFVIYDM NSLMMGEDKI KFKHITPLQE
310 320 330 340 350
QSKEVAIRIF QGCQFRSVEA VQEITEYAKN IPGFINLDLN DQVTLLKYGV
360 370 380 390 400
HEIIYTMLAS LMNKDGVLIS EGQGFMTREF LKNLRKPFGD FMEPKFEFAV
410 420 430 440 450
KFNALELDDS DLAIFIAVII LSGDRPGLLN VKPIEDIQDN LLQALELQLK
460 470 480 490 500
LNHPESSQLF AKVLQKMTDL RQIVTEHVQL LHVIKKTETD MSLHPLLQEI

YKDLY
Length:505
Mass (Da):57,598
Last modified:April 27, 2001 - v3
Checksum:iAB8F3F6086E2A10A
GO
Isoform 1 (identifier: P37238-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:475
Mass (Da):54,512
Checksum:iFD80BD50D9197D3F
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q6GU14Q6GU14_MOUSE
Peroxisome proliferator-activated r...
Pparg PPARG
505Annotation score:
M1VPI1M1VPI1_MOUSE
Peroxisome proliferator-activated r...
Pparg PPARG
475Annotation score:
A0A0N4SV67A0A0N4SV67_MOUSE
Peroxisome proliferator-activated r...
Pparg PPARG
222Annotation score:
A0A0N4SVF8A0A0N4SVF8_MOUSE
Peroxisome proliferator-activated r...
Pparg PPARG
96Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213 – 214MP → DR in AAA62110 (PubMed:8240342).Curated2
Sequence conflicti281 – 283NSL → SSF in AAA62110 (PubMed:8240342).Curated3
Sequence conflicti383N → S in AAA62110 (PubMed:8240342).Curated1
Sequence conflicti383N → S in AAA19971 (PubMed:8041794).Curated1
Sequence conflicti497L → F in AAA62110 (PubMed:8240342).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0036471 – 30Missing in isoform 1. 3 PublicationsAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09138 mRNA Translation: AAA62277.1
U01664 mRNA Translation: AAA62110.1
U01841 mRNA Translation: AAC52134.1
U10374 mRNA Translation: AAA19971.1
CCDSiCCDS20439.1 [P37238-1]
CCDS51876.1 [P37238-2]
PIRiA54101
RefSeqiNP_001120802.1, NM_001127330.2
NP_001295281.1, NM_001308352.1
NP_001295283.1, NM_001308354.1
NP_035276.2, NM_011146.3
XP_006505806.1, XM_006505743.3
XP_011239554.1, XM_011241252.1
XP_017176944.1, XM_017321455.1
UniGeneiMm.3020

Genome annotation databases

GeneIDi19016
KEGGimmu:19016

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09138 mRNA Translation: AAA62277.1
U01664 mRNA Translation: AAA62110.1
U01841 mRNA Translation: AAC52134.1
U10374 mRNA Translation: AAA19971.1
CCDSiCCDS20439.1 [P37238-1]
CCDS51876.1 [P37238-2]
PIRiA54101
RefSeqiNP_001120802.1, NM_001127330.2
NP_001295281.1, NM_001308352.1
NP_001295283.1, NM_001308354.1
NP_035276.2, NM_011146.3
XP_006505806.1, XM_006505743.3
XP_011239554.1, XM_011241252.1
XP_017176944.1, XM_017321455.1
UniGeneiMm.3020

3D structure databases

ProteinModelPortaliP37238
SMRiP37238
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202320, 75 interactors
ComplexPortaliCPX-703 PPARgamma-NCOA2 activated nuclear receptor complex
CPX-864 PPARgamma-NCOA1 activated nuclear receptor complex
CORUMiP37238
DIPiDIP-60435N
ELMiP37238
IntActiP37238, 6 interactors
STRINGi10090.ENSMUSP00000000450

Chemistry databases

BindingDBiP37238
ChEMBLiCHEMBL2459
SwissLipidsiSLP:000001625

PTM databases

iPTMnetiP37238
PhosphoSitePlusiP37238

Proteomic databases

PaxDbiP37238
PRIDEiP37238

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi19016
KEGGimmu:19016

Organism-specific databases

CTDi5468
MGIiMGI:97747 Pparg

Phylogenomic databases

eggNOGiKOG3575 Eukaryota
ENOG410XRZC LUCA
HOGENOMiHOG000261626
HOVERGENiHBG106004
InParanoidiP37238
KOiK08530
PhylomeDBiP37238

Miscellaneous databases

PROiPR:P37238
SOURCEiSearch...

Gene expression databases

CleanExiMM_PPARG

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR003074 1Cnucl_rcpt
IPR035500 NHR_like_dom_sf
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR003077 PPAR-gamma
IPR022590 PPARgamma_N
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00104 Hormone_recep, 1 hit
PF12577 PPARgamma_N, 1 hit
PF00105 zf-C4, 1 hit
PRINTSiPR01288 PROXISOMEPAR
PR01291 PROXISOMPAGR
PR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 1 hit
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPARG_MOUSE
AccessioniPrimary (citable) accession number: P37238
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: April 27, 2001
Last modified: November 7, 2018
This is version 198 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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