UniProtKB - P37230 (PPARA_RAT)
Peroxisome proliferator-activated receptor alpha
Ppara
Functioni
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2 (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 433 | Essential for heterodimerization with RXRABy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 99 – 173 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 75 | |
Zinc fingeri | 102 – 122 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 139 – 161 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor binding Source: RGD
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: RGD
- lipid binding Source: UniProtKB
- MDM2/MDM4 family protein binding Source: RGD
- NFAT protein binding Source: RGD
- nuclear receptor activity Source: RGD
- phosphatase binding Source: RGD
- protein-containing complex binding Source: RGD
- protein domain specific binding Source: RGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- sequence-specific DNA binding Source: RGD
- signaling receptor activity Source: RGD
- steroid hormone receptor activity Source: RGD
- transcription coactivator binding Source: RGD
- ubiquitin conjugating enzyme binding Source: RGD
- zinc ion binding Source: InterPro
GO - Biological processi
- behavioral response to nicotine Source: RGD
- cell differentiation Source: GO_Central
- cellular response to starvation Source: RGD
- circadian regulation of gene expression Source: UniProtKB
- enamel mineralization Source: RGD
- epidermis development Source: RGD
- fatty acid metabolic process Source: RGD
- heart development Source: RGD
- hormone-mediated signaling pathway Source: GO_Central
- lipid localization Source: RGD
- lipoprotein metabolic process Source: RGD
- negative regulation of appetite Source: UniProtKB
- negative regulation of blood pressure Source: RGD
- negative regulation of cell growth involved in cardiac muscle cell development Source: RGD
- negative regulation of cholesterol storage Source: RGD
- negative regulation of cytokine production involved in inflammatory response Source: RGD
- negative regulation of glycolytic process Source: RGD
- negative regulation of hepatocyte apoptotic process Source: RGD
- negative regulation of inflammatory response Source: RGD
- negative regulation of leukocyte cell-cell adhesion Source: RGD
- negative regulation of macrophage derived foam cell differentiation Source: RGD
- negative regulation of miRNA transcription Source: RGD
- negative regulation of neuron death Source: RGD
- negative regulation of protein binding Source: RGD
- negative regulation of protein kinase B signaling Source: RGD
- negative regulation of reactive oxygen species biosynthetic process Source: RGD
- negative regulation of sequestering of triglyceride Source: RGD
- negative regulation of signaling receptor activity Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- negative regulation of transforming growth factor beta receptor signaling pathway Source: RGD
- positive regulation of ATP biosynthetic process Source: RGD
- positive regulation of fatty acid metabolic process Source: GO_Central
- positive regulation of fatty acid oxidation Source: RGD
- positive regulation of gluconeogenesis Source: RGD
- positive regulation of lipid biosynthetic process Source: RGD
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of viral genome replication Source: RGD
- regulation of cellular ketone metabolic process Source: RGD
- regulation of circadian rhythm Source: UniProtKB
- regulation of fatty acid metabolic process Source: RGD
- regulation of gene expression Source: RGD
- regulation of lipid metabolic process Source: GO_Central
- regulation of lipid storage Source: RGD
- regulation of transcription, DNA-templated Source: RGD
- response to ethanol Source: RGD
- response to hypoxia Source: HGNC-UCL
- response to insulin Source: RGD
- response to lipid Source: GO_Central
- response to organic cyclic compound Source: RGD
- wound healing Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding, Receptor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Ligand | Lipid-binding, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-383280, Nuclear Receptor transcription pathway R-RNO-400206, Regulation of lipid metabolism by PPARalpha R-RNO-4090294, SUMOylation of intracellular receptors R-RNO-9707564, Cytoprotection by HMOX1 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxisome proliferator-activated receptor alphaShort name: PPAR-alpha Alternative name(s): Nuclear receptor subfamily 1 group C member 1 |
Gene namesi | Name:Ppara Synonyms:Nr1c1, Ppar |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3369, Ppara |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053484 | 1 – 468 | Peroxisome proliferator-activated receptor alphaAdd BLAST | 468 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P37230 |
PTM databases
iPTMneti | P37230 |
PhosphoSitePlusi | P37230 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000021463, Expressed in liver and 20 other tissues |
Genevisiblei | P37230, RN |
Interactioni
Subunit structurei
Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity.
Interacts with NCOA3 coactivator (By similarity).
Interacts with CITED2; the interaction stimulates its transcriptional activity (PubMed:15051727).
Also interacts with PPARBP in vitro.
Interacts with AKAP13, LPIN1, PRDM16 and coactivator NCOA6.
Interacts with ASXL1 and ASXL2.
Interacts with PER2.
Interacts with SIRT1; the interaction seems to be modulated by NAD+ levels (By similarity).
Interacts with CRY1 and CRY2 (By similarity).
By similarity1 PublicationBinary interactionsi
P37230
With | #Exp. | IntAct |
---|---|---|
Calm3 [P62161] | 2 | EBI-15674997,EBI-397530 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: RGD
- MDM2/MDM4 family protein binding Source: RGD
- NFAT protein binding Source: RGD
- phosphatase binding Source: RGD
- protein domain specific binding Source: RGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- transcription coactivator binding Source: RGD
- ubiquitin conjugating enzyme binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247775, 5 interactors |
CORUMi | P37230 |
DIPi | DIP-29529N |
IntActi | P37230, 1 interactor |
STRINGi | 10116.ENSRNOP00000038651 |
Chemistry databases
BindingDBi | P37230 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 239 – 466 | NR LBDPROSITE-ProRule annotationAdd BLAST | 228 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 304 – 433 | Required for heterodimerization with RXRABy similarityAdd BLAST | 130 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 102 – 122 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 139 – 161 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000157097 |
HOGENOMi | CLU_007368_4_1_1 |
InParanoidi | P37230 |
OMAi | EMGSIQE |
OrthoDBi | 1240230at2759 |
PhylomeDBi | P37230 |
TreeFami | TF316304 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR003074, 1Cnucl_rcpt IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR003076, PPAR-alpha IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01288, PROXISOMEPAR PR01289, PROXISOMPAAR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVDTESPICP LSPLEADDLE SPLSEEFLQE MGNIQEISQS LGEESSGSFS
60 70 80 90 100
FADYQYLGSC PGSEGSVITD TLSPASSPSS VSCPAVPTST DESPGNALNI
110 120 130 140 150
ECRICGDKAS GYHYGVHACE GCKGFFRRTI RLKLAYDKCD RSCKIQKKNR
160 170 180 190 200
NKCQYCRFHK CLSVGMSHNA IRFGRMPRSE KAKLKAEILT CEHDLKDSET
210 220 230 240 250
ADLKSLAKRI HEAYLKNFNM NKVKARVILA GKTSNNPPFV IHDMETLCMA
260 270 280 290 300
EKTLVAKMVA NGVENKEAEV RFFHCCQCMS VETVTELTEF AKAIPGFANL
310 320 330 340 350
DLNDQVTLLK YGVYEAIFTM LSSLMNKDGM LIAYGNGFIT REFLKNLRKP
360 370 380 390 400
FCDIMEPKFD FAMKFNALEL DDSDISLFVA AIICCGDRPG LLNIGYIEKL
410 420 430 440 450
QEGIVHVLKL HLQSNHPDDT FLFPKLLQKM VDLRQLVTEH AQLVQVIKKT
460
ESDAALHPLL QEIYRDMY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88592 mRNA Translation: AAA41918.1 |
PIRi | A45288 |
RefSeqi | NP_037328.1, NM_013196.1 XP_006242218.1, XM_006242156.2 XP_017450169.1, XM_017594680.1 XP_017450170.1, XM_017594681.1 |
Genome annotation databases
Ensembli | ENSRNOT00000030082; ENSRNOP00000038651; ENSRNOG00000021463 |
GeneIDi | 25747 |
KEGGi | rno:25747 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88592 mRNA Translation: AAA41918.1 |
PIRi | A45288 |
RefSeqi | NP_037328.1, NM_013196.1 XP_006242218.1, XM_006242156.2 XP_017450169.1, XM_017594680.1 XP_017450170.1, XM_017594681.1 |
3D structure databases
AlphaFoldDBi | P37230 |
SMRi | P37230 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247775, 5 interactors |
CORUMi | P37230 |
DIPi | DIP-29529N |
IntActi | P37230, 1 interactor |
STRINGi | 10116.ENSRNOP00000038651 |
Chemistry databases
BindingDBi | P37230 |
ChEMBLi | CHEMBL2129 |
PTM databases
iPTMneti | P37230 |
PhosphoSitePlusi | P37230 |
Proteomic databases
PaxDbi | P37230 |
Genome annotation databases
Ensembli | ENSRNOT00000030082; ENSRNOP00000038651; ENSRNOG00000021463 |
GeneIDi | 25747 |
KEGGi | rno:25747 |
Organism-specific databases
CTDi | 5465 |
RGDi | 3369, Ppara |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000157097 |
HOGENOMi | CLU_007368_4_1_1 |
InParanoidi | P37230 |
OMAi | EMGSIQE |
OrthoDBi | 1240230at2759 |
PhylomeDBi | P37230 |
TreeFami | TF316304 |
Enzyme and pathway databases
Reactomei | R-RNO-383280, Nuclear Receptor transcription pathway R-RNO-400206, Regulation of lipid metabolism by PPARalpha R-RNO-4090294, SUMOylation of intracellular receptors R-RNO-9707564, Cytoprotection by HMOX1 |
Miscellaneous databases
PROi | PR:P37230 |
Gene expression databases
Bgeei | ENSRNOG00000021463, Expressed in liver and 20 other tissues |
Genevisiblei | P37230, RN |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR003074, 1Cnucl_rcpt IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR003076, PPAR-alpha IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01288, PROXISOMEPAR PR01289, PROXISOMPAAR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPARA_RAT | |
Accessioni | P37230Primary (citable) accession number: P37230 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 195 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families