UniProtKB - P37201 (NCPR_CANTR)
Protein
NADPH--cytochrome P450 reductase
Gene
NCP1
Organism
Candida tropicalis (Yeast)
Status
Functioni
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation
Catalytic activityi
- EC:1.6.2.4UniRule annotation
Cofactori
Protein has several cofactor binding sites:- FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
- FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 187 | FMNUniRule annotation | 1 | |
Binding sitei | 283 | NADPUniRule annotation | 1 | |
Binding sitei | 537 | NADPUniRule annotation | 1 | |
Binding sitei | 642 | NADPUniRule annotation | 1 | |
Binding sitei | 680 | FADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 66 – 71 | FMNUniRule annotation | 6 | |
Nucleotide bindingi | 117 – 120 | FMNUniRule annotation | 4 | |
Nucleotide bindingi | 152 – 161 | FMNUniRule annotation | 10 | |
Nucleotide bindingi | 439 – 442 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 457 – 459 | FADUniRule annotation | 3 | |
Nucleotide bindingi | 473 – 476 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 599 – 600 | NADPUniRule annotation | 2 | |
Nucleotide bindingi | 606 – 610 | NADPUniRule annotation | 5 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: UniProtKB-UniRule
- FMN binding Source: UniProtKB-UniRule
- NADP binding Source: UniProtKB-UniRule
- NADPH-hemoprotein reductase activity Source: UniProtKB-UniRule
GO - Biological processi
- ergosterol biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | FAD, Flavoprotein, FMN, NADP |
Enzyme and pathway databases
SABIO-RKi | P37201 |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)Short name: CPRUniRule annotation Short name: P450RUniRule annotation |
Gene namesi | Name:NCP1UniRule annotation Synonyms:CPR |
Organismi | Candida tropicalis (Yeast) |
Taxonomic identifieri | 5482 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Candida/Lodderomyces clade › Candida |
Subcellular locationi
Plasma membrane
- Cell membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Mitochondrion
- Mitochondrion outer membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | LumenalUniRule annotation | 5 | |
Transmembranei | 6 – 23 | HelicalUniRule annotationAdd BLAST | 18 | |
Topological domaini | 24 – 680 | CytoplasmicUniRule annotationAdd BLAST | 657 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000167606 | 1 – 680 | NADPH--cytochrome P450 reductaseAdd BLAST | 680 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 60 – 204 | Flavodoxin-likeUniRule annotationAdd BLAST | 145 | |
Domaini | 264 – 509 | FAD-binding FR-typeUniRule annotationAdd BLAST | 246 |
Sequence similaritiesi
Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212, NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR023208, P450R IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208, P450R, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
P37201-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALDKLDLYV IITLVVAIAA YFAKNQFLDQ QQDTGFLNTD SGDGNSRDIL
60 70 80 90 100
QALKKNNKNT LLLFGSQTGT AEDYANKLSR ELHSRFGLKT MVADFADYDF
110 120 130 140 150
ENFGDITEDI LVFFIVATYG EGEPTDNADE FHTWLTEEAD TLSTLKYTVF
160 170 180 190 200
GLGNSTYEFF NAIGRKFDRL LGEKGGDRFA EYGEGDDGTG TLDEDFLAWK
210 220 230 240 250
DNVFDSLKND LNFEEKELKY EPNVKLTERD DLSGNDPDVS LGEPNVKYIK
260 270 280 290 300
SEGVDLTKGP FDHTHPFLAR IVKTKELFTS EDRHCVHVEF DISESNLKYT
310 320 330 340 350
TGDHLAIWPS NSDENIKQFA KCFGLEDKLD TVIELKALDS TYSIPFPNPI
360 370 380 390 400
TYGAVIRHHL EISGPVSRQF FLSIAGFAPD EETKKSFTRI GGDKQEFASK
410 420 430 440 450
VTRRKFNIAD ALLFASNNRP WSDVPFEFLI ENVQHLTPRY YSISSSSLSE
460 470 480 490 500
KQTINVTAVV EAEEEADGRP VTGVVTNLLK NIEIEQNKTG ETPMVHYDLN
510 520 530 540 550
GPRGKFSKFR LPVHVRRSNF KLPKNSTTPV ILIGPGTGVA PLRGFVRERV
560 570 580 590 600
QQVKNGVNVG KTVLFYGCRN SEQDFLYKQE WSEYASVLGE NFEMFNAFSR
610 620 630 640 650
QDPTKKVYVQ DKILENSALV DELLSSGAII YVCGDASRMA RDVQAAIAKI
660 670 680
VAKSRDIHED KAAELVKSWK VQNRYQEDVW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35199 Genomic DNA Translation: AAA34333.1 |
PIRi | A37890 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35199 Genomic DNA Translation: AAA34333.1 |
PIRi | A37890 |
3D structure databases
SMRi | P37201 |
ModBasei | Search... |
Enzyme and pathway databases
SABIO-RKi | P37201 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212, NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR023208, P450R IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208, P450R, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCPR_CANTR | |
Accessioni | P37201Primary (citable) accession number: P37201 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | August 12, 2020 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |