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Protein

PTS system galactitol-specific EIIB component

Gene

gatB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. The enzyme II complex composed of GatA, GatB and GatC is involved in galactitol transport. It can also use D-glucitol.2 Publications

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + galactitol(Side 1) = [protein]-L-histidine + galactitol 1-phosphate(Side 2).1 Publication

Kineticsi

  1. KM=4.4 µM for D-galactitol1 Publication
  2. KM=800 µM for D-glucitol1 Publication
  1. Vmax=3.2 nmol/min/mg enzyme with D-galactitol as substrate1 Publication
  2. Vmax=3.2 nmol/min/mg enzyme with D-glucitol as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9Phosphocysteine intermediate; for EIIB activityCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGalactitol metabolism, Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GATB-MONOMER
MetaCyc:GATB-MONOMER

Protein family/group databases

TCDBi4.A.5.1.1 the pts galactitol (gat) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system galactitol-specific EIIB component1 Publication
Alternative name(s):
EIIB-Gat1 Publication
Galactitol-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.2001 Publication)
Gene namesi
Name:gatB1 Publication
Ordered Locus Names:b2093, JW2077
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12415 gatB

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865721 – 94PTS system galactitol-specific EIIB componentAdd BLAST94

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Phosphocysteine; by EIIACurated1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP37188
PRIDEiP37188

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Subunit structurei

Forms a complex with one each of subunit of GatA, GatB and 2 subunits of GatC.1 Publication

Protein-protein interaction databases

BioGridi4263510, 11 interactors
DIPiDIP-9744N
IntActiP37188, 7 interactors
STRINGi316385.ECDH10B_2246

Structurei

3D structure databases

ProteinModelPortaliP37188
SMRiP37188
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 94PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST94

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41090UW Bacteria
COG3414 LUCA
HOGENOMiHOG000230475
KOiK02774
PhylomeDBiP37188

Family and domain databases

InterProiView protein in InterPro
IPR036095 PTS_EIIB-like_sf
IPR013011 PTS_EIIB_2
IPR003501 PTS_EIIB_2/3
PfamiView protein in Pfam
PF02302 PTS_IIB, 1 hit
SUPFAMiSSF52794 SSF52794, 1 hit
PROSITEiView protein in PROSITE
PS51099 PTS_EIIB_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P37188-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKRKIIVACG GAVATSTMAA EEIKELCQNH NIPVELIQCR VNEIETYMDG
60 70 80 90
VHLICTTAKV DRSFGDIPLV HGMPFISGIG IEALQNKILT ILQG
Length:94
Mass (Da):10,222
Last modified:November 1, 1997 - v2
Checksum:iA8156F04894F0CD7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9C → E AA sequence (PubMed:9868784).Curated1
Sequence conflicti29N → S in CAA56229 (PubMed:7772602).Curated1
Sequence conflicti59K → R in CAA56229 (PubMed:7772602).Curated1
Sequence conflicti76I → V in CAA56229 (PubMed:7772602).Curated1
Sequence conflicti79I → V in CAA56229 (PubMed:7772602).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA Translation: CAA56229.1
U00096 Genomic DNA Translation: AAC75154.1
AP009048 Genomic DNA Translation: BAA15956.1
PIRiD64976
S55904
RefSeqiNP_416596.1, NC_000913.3
WP_000823270.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75154; AAC75154; b2093
BAA15956; BAA15956; BAA15956
GeneIDi946610
KEGGiecj:JW2077
eco:b2093
PATRICifig|1411691.4.peg.157

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79837 Genomic DNA Translation: CAA56229.1
U00096 Genomic DNA Translation: AAC75154.1
AP009048 Genomic DNA Translation: BAA15956.1
PIRiD64976
S55904
RefSeqiNP_416596.1, NC_000913.3
WP_000823270.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP37188
SMRiP37188
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263510, 11 interactors
DIPiDIP-9744N
IntActiP37188, 7 interactors
STRINGi316385.ECDH10B_2246

Protein family/group databases

TCDBi4.A.5.1.1 the pts galactitol (gat) family

Proteomic databases

PaxDbiP37188
PRIDEiP37188

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75154; AAC75154; b2093
BAA15956; BAA15956; BAA15956
GeneIDi946610
KEGGiecj:JW2077
eco:b2093
PATRICifig|1411691.4.peg.157

Organism-specific databases

EchoBASEiEB2314
EcoGeneiEG12415 gatB

Phylogenomic databases

eggNOGiENOG41090UW Bacteria
COG3414 LUCA
HOGENOMiHOG000230475
KOiK02774
PhylomeDBiP37188

Enzyme and pathway databases

BioCyciEcoCyc:GATB-MONOMER
MetaCyc:GATB-MONOMER

Miscellaneous databases

PROiPR:P37188

Family and domain databases

InterProiView protein in InterPro
IPR036095 PTS_EIIB-like_sf
IPR013011 PTS_EIIB_2
IPR003501 PTS_EIIB_2/3
PfamiView protein in Pfam
PF02302 PTS_IIB, 1 hit
SUPFAMiSSF52794 SSF52794, 1 hit
PROSITEiView protein in PROSITE
PS51099 PTS_EIIB_TYPE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTKB_ECOLI
AccessioniPrimary (citable) accession number: P37188
Secondary accession number(s): P76412
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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