UniProtKB - P37182 (HYBD_ECOLI)
Protein
Hydrogenase 2 maturation protease
Gene
hybD
Organism
Escherichia coli (strain K12)
Status
Functioni
Protease involved in the C-terminal processing of HybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of HybC.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 16 | Nickel | 1 | |
Metal bindingi | 62 | Nickel | 1 | |
Metal bindingi | 93 | Nickel | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
- endopeptidase activity Source: GO_Central
- enzyme activator activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cellular protein modification process Source: GO_Central
- protein processing Source: EcoCyc
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Ligand | Metal-binding, Nickel |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11802-MONOMER |
BRENDAi | 3.4.23.B20, 2026 |
Protein family/group databases
MEROPSi | A31.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hybD Ordered Locus Names:b2993, JW2961 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201943 | 1 – 164 | Hydrogenase 2 maturation proteaseAdd BLAST | 164 |
Proteomic databases
jPOSTi | P37182 |
PaxDbi | P37182 |
PRIDEi | P37182 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262374, 54 interactors 853231, 1 interactor |
DIPi | DIP-9967N |
IntActi | P37182, 6 interactors |
STRINGi | 511145.b2993 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P37182 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P37182 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase A31 family.Curated
Phylogenomic databases
eggNOGi | COG0680, Bacteria |
HOGENOMi | CLU_099037_0_0_6 |
InParanoidi | P37182 |
PhylomeDBi | P37182 |
Family and domain databases
Gene3Di | 3.40.50.1450, 1 hit |
InterProi | View protein in InterPro IPR004419, Pept_A31_hyd_express IPR023430, Pept_HybD-like_dom_sf IPR000671, Peptidase_A31 |
PANTHERi | PTHR30302, PTHR30302, 1 hit |
Pfami | View protein in Pfam PF01750, HycI, 1 hit |
PRINTSi | PR00446, HYDRGNUPTAKE |
SUPFAMi | SSF53163, SSF53163, 1 hit |
TIGRFAMsi | TIGR00140, hupD, 1 hit TIGR00072, hydrog_prot, 1 hit |
i Sequence
Sequence statusi: Complete.
P37182-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRILVLGVGN ILLTDEAIGV RIVEALEQRY ILPDYVEILD GGTAGMELLG
60 70 80 90 100
DMANRDHLII ADAIVSKKNA PGTMMILRDE EVPALFTNKI SPHQLGLADV
110 120 130 140 150
LSALRFTGEF PKKLTLVGVI PESLEPHIGL TPTVEAMIEP ALEQVLAALR
160
ESGVEAIPRE AIHD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 160 – 164 | EAIHD → SDS in AAA21592 (PubMed:8021226).Curated | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09177 Genomic DNA Translation: AAA21592.1 U28377 Genomic DNA Translation: AAA69160.1 U00096 Genomic DNA Translation: AAC76029.1 AP009048 Genomic DNA Translation: BAE77054.1 |
PIRi | G65085 |
RefSeqi | NP_417467.1, NC_000913.3 WP_001221939.1, NZ_SSZK01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC76029; AAC76029; b2993 BAE77054; BAE77054; BAE77054 |
GeneIDi | 948982 |
KEGGi | ecj:JW2961 eco:b2993 |
PATRICi | fig|1411691.4.peg.3736 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09177 Genomic DNA Translation: AAA21592.1 U28377 Genomic DNA Translation: AAA69160.1 U00096 Genomic DNA Translation: AAC76029.1 AP009048 Genomic DNA Translation: BAE77054.1 |
PIRi | G65085 |
RefSeqi | NP_417467.1, NC_000913.3 WP_001221939.1, NZ_SSZK01000023.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CFZ | X-ray | 2.20 | A/B/C/D/E/F | 1-159 | [»] | |
SMRi | P37182 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262374, 54 interactors 853231, 1 interactor |
DIPi | DIP-9967N |
IntActi | P37182, 6 interactors |
STRINGi | 511145.b2993 |
Protein family/group databases
MEROPSi | A31.001 |
Proteomic databases
jPOSTi | P37182 |
PaxDbi | P37182 |
PRIDEi | P37182 |
Genome annotation databases
EnsemblBacteriai | AAC76029; AAC76029; b2993 BAE77054; BAE77054; BAE77054 |
GeneIDi | 948982 |
KEGGi | ecj:JW2961 eco:b2993 |
PATRICi | fig|1411691.4.peg.3736 |
Organism-specific databases
EchoBASEi | EB1750 |
Phylogenomic databases
eggNOGi | COG0680, Bacteria |
HOGENOMi | CLU_099037_0_0_6 |
InParanoidi | P37182 |
PhylomeDBi | P37182 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11802-MONOMER |
BRENDAi | 3.4.23.B20, 2026 |
Miscellaneous databases
EvolutionaryTracei | P37182 |
PROi | PR:P37182 |
Family and domain databases
Gene3Di | 3.40.50.1450, 1 hit |
InterProi | View protein in InterPro IPR004419, Pept_A31_hyd_express IPR023430, Pept_HybD-like_dom_sf IPR000671, Peptidase_A31 |
PANTHERi | PTHR30302, PTHR30302, 1 hit |
Pfami | View protein in Pfam PF01750, HycI, 1 hit |
PRINTSi | PR00446, HYDRGNUPTAKE |
SUPFAMi | SSF53163, SSF53163, 1 hit |
TIGRFAMsi | TIGR00140, hupD, 1 hit TIGR00072, hydrog_prot, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HYBD_ECOLI | |
Accessioni | P37182Primary (citable) accession number: P37182 Secondary accession number(s): Q2M9K2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families