UniProtKB - P37136 (ACES_RAT)
Acetylcholinesterase
Ache
Functioni
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 234 | Acyl-ester intermediatePROSITE-ProRule annotation | 1 | |
Active sitei | 365 | Charge relay systemBy similarity | 1 | |
Active sitei | 478 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- acetylcholine binding Source: RGD
- acetylcholinesterase activity Source: RGD
- carboxylic ester hydrolase activity Source: GO_Central
- choline binding Source: RGD
- cholinesterase activity Source: RGD
- collagen binding Source: RGD
- hydrolase activity Source: RGD
- identical protein binding Source: RGD
- laminin binding Source: RGD
- protein homodimerization activity Source: RGD
- protein self-association Source: RGD
- serine hydrolase activity Source: RGD
GO - Biological processi
- acetylcholine catabolic process Source: RGD
- acetylcholine catabolic process in synaptic cleft Source: SynGO
- acetylcholine metabolic process Source: RGD
- acetylcholine receptor signaling pathway Source: RGD
- cell adhesion Source: RGD
- choline metabolic process Source: RGD
- neuron death Source: RGD
- osteoblast development Source: RGD
- positive regulation of axonogenesis Source: RGD
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of dendrite morphogenesis Source: RGD
- receptor internalization Source: RGD
- regulation of receptor recycling Source: RGD
- response to antibiotic Source: RGD
- response to insulin Source: RGD
- retina development in camera-type eye Source: RGD
- synapse assembly Source: RGD
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Neurotransmitter degradation |
Enzyme and pathway databases
BRENDAi | 3.1.1.7, 5301 |
Protein family/group databases
ESTHERi | ratno-ACHE, AChE |
MEROPSi | S09.979 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ache |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69313, Ache |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Extracellular region or secreted
Other locations
Plasma membrane
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: RGD
- rough endoplasmic reticulum Source: RGD
Extracellular region or secreted
- extracellular region Source: RGD
- extracellular space Source: RGD
- synaptic cleft Source: SynGO
Golgi apparatus
- Golgi apparatus Source: RGD
Nucleus
- nuclear envelope Source: RGD
Plasma Membrane
- plasma membrane Source: RGD
- postsynaptic membrane Source: RGD
- presynaptic membrane Source: RGD
Other locations
- anchored component of membrane Source: UniProtKB-KW
- axon Source: RGD
- basement membrane Source: RGD
- cell surface Source: RGD
- cytoplasm Source: RGD
- dendrite Source: RGD
- membrane Source: RGD
- neuromuscular junction Source: SynGO
- neuron projection Source: RGD
- neuronal cell body Source: RGD
- perinuclear region of cytoplasm Source: RGD
- synapse Source: RGD
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Secreted, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
ChainiPRO_0000008590 | 32 – 614 | AcetylcholinesteraseAdd BLAST | 583 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 100 ↔ 127 | By similarity | ||
Disulfide bondi | 288 ↔ 303 | By similarity | ||
Glycosylationi | 296 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 381 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 440 ↔ 560 | By similarity | ||
Glycosylationi | 495 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 611 | InterchainBy similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PRIDEi | P37136 |
PTM databases
GlyGeni | P37136, 3 sites |
iPTMneti | P37136 |
PhosphoSitePlusi | P37136 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homotetramer; composed of disulfide-linked homodimers. Catalytic forms H (GPI-anchor dimer) and T (asymmetric collagen-tailed), which differ in their C-terminus, account for all types of known ACHE forms (Probable).
Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers (By similarity).
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: RGD
- laminin binding Source: RGD
- protein homodimerization activity Source: RGD
- protein self-association Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000064185 |
Chemistry databases
BindingDBi | P37136 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4389, Eukaryota |
InParanoidi | P37136 |
OrthoDBi | 754103at2759 |
PhylomeDBi | P37136 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR014788, AChE_tetra IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF08674, AChE_tetra, 1 hit PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPPWYPLHT PSLASPLLFL LLSLLGGGAR AEGREDPQLL VRVRGGQLRG
60 70 80 90 100
IRLKAPGGPV SAFLGIPFAE PPVGSRRFMP PEPKRPWSGI LDATTFQNVC
110 120 130 140 150
YQYVDTLYPG FEGTEMWNPN RELSEDCLYL NVWTPYPRPT SPTPVLIWIY
160 170 180 190 200
GGGFYSGASS LDVYDGRFLA QVEGTVLVSM NYRVGTFGFL ALPGSREAPG
210 220 230 240 250
NVGLLDQRLA LQWVQENIAA FGGDPMSVTL FGESAGAASV GMHILSLPSR
260 270 280 290 300
SLFHRAVLQS GTPNGPWATV SAGEARRRAT LLARLVGCPP GGAGGNDTEL
310 320 330 340 350
ISCLRTRPAQ DLVDHEWHVL PQESIFRFSF VPVVDGDFLS DTPDALINTG
360 370 380 390 400
DFQDLQVLVG VVKDEGSYFL VYGVPGFSKD NESLISRAQF LAGVRIGVPQ
410 420 430 440 450
ASDLAAEAVV LHYTDWLHPE DPAHLRDAMS AVVGDHNVVC PVAQLAGRLA
460 470 480 490 500
AQGARVYAYI FEHRASTLTW PLWMGVPHGY EIEFIFGLPL DPSLNYTVEE
510 520 530 540 550
RIFAQRLMQY WTNFARTGDP NDPRDSKSPR WPPYTTAAQQ YVSLNLKPLE
560 570 580 590 600
VRRGLRAQTC AFWNRFLPKL LSATDTLDEA ERQWKAEFHR WSSYMVHWKN
610
QFDHYSKQER CSDL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0R4A5 | M0R4A5_RAT | Carboxylic ester hydrolase | Ache | 616 | Annotation score: | ||
Q505J2 | Q505J2_RAT | Carboxylic ester hydrolase | Ache rCG_21937 | 614 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001458 | 575 – 614 | DTLDE…RCSDL → ATEVPCTCPSPAHGEAAPRP GPALSLSLLFFLFLLHSGLR WL in isoform H. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_001459 | 575 – 614 | DTLDE…RCSDL → GRRGVGKQGMHKAARVGRTG ERKGGKHRM in isoform R. 1 PublicationAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S50879 mRNA Translation: AAB24586.1 X70140 mRNA Translation: CAA49717.1 X70141 mRNA Translation: CAA49718.1 |
PIRi | JH0811 |
RefSeqi | NP_742006.1, NM_172009.1 [P37136-1] |
Genome annotation databases
GeneIDi | 83817 |
KEGGi | rno:83817 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S50879 mRNA Translation: AAB24586.1 X70140 mRNA Translation: CAA49717.1 X70141 mRNA Translation: CAA49718.1 |
PIRi | JH0811 |
RefSeqi | NP_742006.1, NM_172009.1 [P37136-1] |
3D structure databases
AlphaFoldDBi | P37136 |
SMRi | P37136 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000064185 |
Chemistry databases
BindingDBi | P37136 |
ChEMBLi | CHEMBL3199 |
DrugCentrali | P37136 |
Protein family/group databases
ESTHERi | ratno-ACHE, AChE |
MEROPSi | S09.979 |
PTM databases
GlyGeni | P37136, 3 sites |
iPTMneti | P37136 |
PhosphoSitePlusi | P37136 |
Proteomic databases
PRIDEi | P37136 |
Genome annotation databases
GeneIDi | 83817 |
KEGGi | rno:83817 |
Organism-specific databases
CTDi | 43 |
RGDi | 69313, Ache |
Phylogenomic databases
eggNOGi | KOG4389, Eukaryota |
InParanoidi | P37136 |
OrthoDBi | 754103at2759 |
PhylomeDBi | P37136 |
Enzyme and pathway databases
BRENDAi | 3.1.1.7, 5301 |
Miscellaneous databases
PROi | PR:P37136 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR014788, AChE_tetra IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF08674, AChE_tetra, 1 hit PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACES_RAT | |
Accessioni | P37136Primary (citable) accession number: P37136 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families