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Protein

Flavonoid 3',5'-hydroxylase

Gene

CYP75A2

Organism
Solanum melongena (Eggplant) (Aubergine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin.By similarity

Catalytic activityi

A flavanone + 2 [reduced NADPH--hemoprotein reductase] + 2 O2 = a 3',5'-dihydroxyflavanone + 2 [oxidized NADPH--hemoprotein reductase] + 2 H2O.By similarity

Cofactori

hemeBy similarity

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi446Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.88 5755
UniPathwayi
UPA00009

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonoid 3',5'-hydroxylase (EC:1.14.14.81By similarity)
Short name:
F3'5'H
Alternative name(s):
CYPLXXVA2
Cytochrome P450 75A2
P-450EG1
Gene namesi
Name:CYP75A2
Synonyms:CYP75, CYPEG1
OrganismiSolanum melongena (Eggplant) (Aubergine)
Taxonomic identifieri4111 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521311 – 513Flavonoid 3',5'-hydroxylaseAdd BLAST513

Expressioni

Tissue specificityi

Hypocotyl tissues.

Developmental stagei

Most abundant during the mid stage of flower bud development but not detected in leaf tissues.

Inductioni

By white light.

Structurei

3D structure databases

ProteinModelPortaliP37120
SMRiP37120
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

P37120-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVILPSELIG ATIIYIIVYI IIQKLIATGS WRRRRLPPGP EGWPVIGALP
60 70 80 90 100
LLGGMPHVAL AKMAKKYGPI MYLKVGTCGM VVASTPNAAK AFLKTLDINF
110 120 130 140 150
SNRPPNAGAT HMAYNAQDMV FAPYGPRWKL LRKLSNLHML GGKALENWAN
160 170 180 190 200
VRANELGHML KSMFDASHVG ERIVVADMLT FAMANMIGQV MLSKRVFVEK
210 220 230 240 250
GKEVNEFKNM VVELMTVAGY FNIGDFIPQI AWMDLQGIEK GMKKLHKKFD
260 270 280 290 300
DLLTKMFEEH EATSNERKGK PDFLDFIMAN RDNSEGERLS ITNIKALLLN
310 320 330 340 350
LFTAGTDTSS SVIEWALTEM MKNPTIFKKA QQEMDQIIGK NRRFIESDIP
360 370 380 390 400
NLPYLRAICK EAFRKHPSTP LNLPRVSSDA CTIDGYYIPK NTRLSVNIWA
410 420 430 440 450
IGRDPDVWEN PLEFIPERFL SEKNAKIEHR GNDFELIPFG AGRRICAGTR
460 470 480 490 500
MGIVMVEYIL GTLIHSFDWK LPNDVVDINM EETFGLALQK AVPLEAIVTP
510
RLSFDIYQSS EPF
Length:513
Mass (Da):57,784
Last modified:October 1, 1994 - v1
Checksum:iD0434B46B68535EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70824 mRNA Translation: CAA50155.1
PIRiS43342

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70824 mRNA Translation: CAA50155.1
PIRiS43342

3D structure databases

ProteinModelPortaliP37120
SMRiP37120
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00009

BRENDAi1.14.13.88 5755

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC75A2_SOLME
AccessioniPrimary (citable) accession number: P37120
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 12, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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