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Protein

NADPH--cytochrome P450 reductase

Gene
N/A
Organism
Vigna radiata var. radiata (Mung bean) (Phaseolus aureus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217FMNUniRule annotation1
Binding sitei309NADPUniRule annotation1
Binding sitei549NADPUniRule annotation1
Binding sitei652NADPUniRule annotation1
Binding sitei690FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 93FMNUniRule annotation6
Nucleotide bindingi143 – 146FMNUniRule annotation4
Nucleotide bindingi182 – 191FMNUniRule annotation10
Nucleotide bindingi468 – 471FADUniRule annotation4
Nucleotide bindingi486 – 488FADUniRule annotation3
Nucleotide bindingi502 – 505FADUniRule annotation4
Nucleotide bindingi610 – 611NADPUniRule annotation2
Nucleotide bindingi616 – 620NADPUniRule annotation5

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
OrganismiVigna radiata var. radiata (Mung bean) (Phaseolus aureus)
Taxonomic identifieri3916 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideae50 kb inversion cladeNPAAA cladeindigoferoid/millettioid cladePhaseoleaeVigna
Proteomesi
  • UP000087766 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25LumenalUniRule annotationAdd BLAST25
Transmembranei26 – 46HelicalUniRule annotationAdd BLAST21
Topological domaini47 – 690CytoplasmicUniRule annotationAdd BLAST644

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001676111 – 690NADPH--cytochrome P450 reductaseAdd BLAST690

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP37116
SMRiP37116
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 233Flavodoxin-likeUniRule annotationAdd BLAST152
Domaini289 – 535FAD-binding FR-typeUniRule annotationAdd BLAST247

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK00327

Family and domain databases

Gene3Di1.20.990.10, 4 hits
3.40.50.360, 1 hit
3.40.50.80, 1 hit
HAMAPiMF_03212 NCPR, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR023208 P450R
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000208 P450R, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

P37116-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASNSDLVRA VESFLGVSLG DSVSDSLLLI ATTSAAVVVG LLVFLWKKSS
60 70 80 90 100
DRSKEVKPVV VPRDLMMEEE EEVDVAAGKT KVTIFFGTQT GTAEGFAKAL
110 120 130 140 150
AEEIKARYEK AAVKVVDLDD YAADDDLYEE KLKKESLVFF MLATYGDGEP
160 170 180 190 200
IDNAARFYKW FTEGKDERGI WLQKLTYGVF GLGNRQYEHF NKIGKVVDEE
210 220 230 240 250
LAEQGAKRLV AVGLGDDDQS IEDDFSAWKE SLWSELDQLL RDEDDANTVS
260 270 280 290 300
TPYTAAILEY RVVIHDPTAA STYDNHSTVA NGNTEFDIHH PCRVNVAVQK
310 320 330 340 350
ELHKPESDRS CIHLEFDISG TSITYDTGDH VGVYAENCNE TVEETGKLLG
360 370 380 390 400
QNLDLFFSLH TDKDDGTSLG GSLLPPFPGP CSLRTALARY ADLLNPPRKA
410 420 430 440 450
ALLALATHAS EPSDERLKFL SSPQGKDEYS KWVVGSQRSL VEVMAEFPSA
460 470 480 490 500
KPPLGVFFAA IAPRLQPRYY SISSSPRFAP QRVHVTCALV YGPTPTGRIH
510 520 530 540 550
KGVCSTWMKN AIPSEKSQDC SSAPIFIRPS NFKLPVDHSI PIIMVGPGTG
560 570 580 590 600
LAPFRGFLQE RYALKEDGVQ LGPALLFFGC RNRQMDFIYE DELKSFVEQG
610 620 630 640 650
SLSELIVAFS REGAEKEYVQ HKMMDKAAHL WSLISQGGYL YVCGDAKGMA
660 670 680 690
RDVHRTLHSI VQEQENVDST KAEAIVKKLQ MDGRYLRDVW
Length:690
Mass (Da):76,506
Last modified:October 1, 1994 - v1
Checksum:i592966167E8561DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07843 mRNA Translation: AAA34240.1
RefSeqiNP_001304239.1, NM_001317310.1

Genome annotation databases

GeneIDi106762177
KEGGivra:106762177

Similar proteinsi

Entry informationi

Entry nameiNCPR_VIGRR
AccessioniPrimary (citable) accession number: P37116
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 12, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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